Kit for determining anti-cyclic citrullinated peptide (Anti-CCP) antibody

An anti-cyclic citrullinated peptide and a kit technology are applied in the field of medical immunology in vitro diagnosis, which can solve the problems of inability to detect and high cost of measurement, and achieve the effects of simple operation, enhanced reaction absorbance, and improved automation.

Active Publication Date: 2012-06-20
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two detection methods require solid phase or electrode support, and both require specific detection instruments, and cannot be directly detected on a fu

Method used

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  • Kit for determining anti-cyclic citrullinated peptide (Anti-CCP) antibody
  • Kit for determining anti-cyclic citrullinated peptide (Anti-CCP) antibody
  • Kit for determining anti-cyclic citrullinated peptide (Anti-CCP) antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0042] The components (percentage by weight) in the R1 reagent are:

[0043] PB buffer 0.4% Inorganic salt 1% TWEEN20 0.05% ProClin300 0.02% Interference scavenging protein (Roche ) 0.02% (Modified) CCP-sensitive nano-PS microspheres 0.1% Ultra-pure water 98.41%

[0044] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitive nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 60nm.

[0045] The components (percentage by weight) in the R2 reagent are:

[0046] PB buffer 0.4% Inorganic salt 1% TWEEN 20 0.05% ProClin300 0.02% Goat anti-human IgG-FC antibody 0.001% Ultra-pure water 98.529%

[0047] The above ingredients are mixed to obtain reagent 2.

[0048] When used, the mixing ratio of R...

Embodiment 2

[0051] Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0052] The components (percentage by weight) in the R1 reagent are:

[0053] PB buffer 5% Inorganic salt 5% Emulgen120 1% Sodium azide 0.1% Interfering scavengers (scantibodies) 0.1% (Modified) CCP-sensitive nano-PS microspheres 0.06% Ultra-pure water 88.74%

[0054] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitive nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 200nm.

[0055] The components (percentage by weight) in the R2 reagent are:

[0056] PB buffer 5% Inorganic salt 5% EMULGEN120 1% Sodium azide 0.1% Goat anti-human IgG-FC antibody 0.3% Ultra-pure water 88.6%

[0057] The above ingredients are mixed to obtain reagent 2.

[0058] When used, the mixing ratio of the R1 rea...

Embodiment 3

[0060]Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0061] The components (percentage by weight) in the R1 reagent are:

[0062] Goods' buffer 10% Inorganic salt 10% TX100 2% Thimerosal 0.2% Interference clearing protein (Roche ) 0.2% (Modified) CCP-sensitive nano-PS microspheres 0.05% Ultra-pure water 77.55%

[0063] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitive nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 500nm.

[0064] The components (percentage by weight) in the R2 reagent are:

[0065] PB buffer 10% Inorganic salt 10% TX100 2% Thimerosal 0.2% Goat anti-human IgG-FC antibody 0.5% Ultra-pure water 77.3%

[0066] The above ingredients are mixed to obtain reagent 2.

[0067] When used, the mixing ratio of the R1 reagent a...

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Abstract

The invention discloses a kit for determining an anti-cyclic citrullinated peptide (Anti-CCP) antibody. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, inorganic salt, surfactant, preservative, interference removing protein, modified CCP-sensitized nano poly styrene (PS) microspheres and ultrapure water; the reagent R2 comprises a buffer solution, inorganic salt, surfactant, preservative, an anti Anti-CCP second antibody and ultrapure water; and the weight ratio of the reagent R1 to the reagent R2 is (50-90):(10-50). According to the method, the Anti-CCP is determined by adding the anti Anti-CCP second antibody by a latex booster immunization transmission turbidimetry, a detection signal is subjected to two-stage amplification, the detection sensitivity is improved, and the determination range is enlarged; and the kit can be applied to an automatic biochemical analyzer to shorten detection time, and improve detection efficiency.

Description

technical field [0001] The invention belongs to the field of in vitro medical immunodiagnosis, and relates to an immunoturbidimetric detection reagent, and further, the invention relates to an anti-cyclic citrullinated peptide antibody detection kit. Background technique [0002] Anti-cyclic citrullinated peptide antibody (Anti-CCP) is a polypeptide fragment of cyclic filaggrin, and it is an IgG-based antibody. Clinical studies have shown that Anti-CCP has good sensitivity and specificity for rheumatoid arthritis (RA), and is related to the activity and progression of RA. Detection of Anti-CCP is very important for the early diagnosis and prognosis evaluation of RA. Significant. [0003] Currently, there are two methods for clinical detection of Anti-CCP: 1. Enzyme-linked Immuno Sorbent Assay (ELISA); 2. Electrochemiluminescence immunoassay (ECLI). These two detection methods require solid phase or electrode support, and both require specific detection instruments, and can...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/82G01N21/31
Inventor 周方银韩帅谭韬周帅何涛涛
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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