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Latex enhanced immuno-nephelometry kit for determining procalcitonin and preparation method and application of latex enhanced immuno-nephelometry kit for determining procalcitonin

A procalcitonin, latex-enhanced technology, used in biological tests, measurement devices, and analysis by chemically reacting materials, which can solve problems such as long time required

Active Publication Date: 2014-12-24
BEIJING MOKOBIO LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage of RIA is that it takes a long time

Method used

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  • Latex enhanced immuno-nephelometry kit for determining procalcitonin and preparation method and application of latex enhanced immuno-nephelometry kit for determining procalcitonin
  • Latex enhanced immuno-nephelometry kit for determining procalcitonin and preparation method and application of latex enhanced immuno-nephelometry kit for determining procalcitonin
  • Latex enhanced immuno-nephelometry kit for determining procalcitonin and preparation method and application of latex enhanced immuno-nephelometry kit for determining procalcitonin

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Embodiment 1

[0040] Example 1 Preparation of a latex-enhanced immune turbidimetric kit for measuring procalcitonin of the present invention

[0041] 1. Reagent configuration

[0042] 1.1. Preparation of diluent R1

[0043] Recipe: 0.05M Tris-HCl buffer containing 2M NaCl, 2% (w / w) PEG20000, 5% (w / w) BSA, 0.1% (w / w) proclin-300, pH=8.4

[0044] 1.2. Preparation of latex reagent R2

[0045] (1) Carboxylated polystyrene latex activation

[0046] Add 50mM to 1ml of carboxylated polystyrene latex of 10mg / ml, 1mg EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and 10mg NHS (N-hydroxysuccinimide) was mixed and activated for 60 minutes, centrifuged at 8000rpm to discard the supernatant, and resuspended with 50mM PBS; according to the above method, the activated carboxylated polyphenylene with a particle size of 76nm and 187nm was prepared respectively. vinyl latex;

[0047] (2) Preparation of carboxylated polystyrene latex coupled with PCT monoclonal antibody

[0048] Add 1...

Embodiment 2

[0079] Example 2 Application of the kit of the present invention in the preparation and detection of procalcitonin reagents

[0080] 1, the using method of kit of the present invention:

[0081] The immunoturbidimetric method is adopted, and the latex-enhanced immune turbidimetric method is used as the determination principle. The PCT in the sample reacts specifically with the latex particles labeled with the PCT antibody to form an immune complex, which causes a change in absorbance, which is proportional to the concentration of PCT in the sample. Make working curve with the PCT standard substance of known concentration, can calculate the PCT content in the sample from this curve, the standard curve that calibrator of the present invention prepares is as follows figure 1 As shown, after the calibration is completed, the quality control is tested first, and its concentration is within the marked range (0.21-0.29μg / L), and the kit is valid.

[0082] 2. Applicable instruments:...

example 1

[0100]

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Abstract

The invention discloses a latex enhanced immuno-nephelometry kit for determining procalcitonin and a preparation method and application of the latex enhanced immuno-nephelometry kit for determining procalcitonin, and belongs to the technical field of disease diagnosis and detection. The latex enhanced immuno-nephelometry kit for determining the procalcitonin comprises a diluent, a latex preparation, a blank liquid, a calibrator and a quality control material, wherein the latex preparation contains carboxylated polystyrene latex with different particle sizes and coupled with a PCT monoclonal antibody and a PCT polyclonal antibody respectively. According to the method, the PCT monoclonal antibody and the PCT polyclonal antibody are marked respectively, the latex with the large particle size can improve the detection sensitivity, and the latex with the small particle size can expand the linearity range, and therefore, the composite latex preparation can improve the detection sensitivity, can expand the detection linearity range, and has the advantages of high detection speed, high sensitivity, strong specificity and good accuracy for detection on the procalcitonin.

Description

technical field [0001] The invention discloses a latex-enhanced immune turbidimetric kit and its preparation method and application, in particular to a latex-enhanced immune turbidimetric kit for measuring procalcitonin and its preparation method and application. The invention belongs to disease in the field of diagnosis or detection technology. Background technique [0002] Procalcitonin (PCT) is a calcitonin propeptide substance without hormonal activity, consisting of 116 amino acids and a glycoprotein with a molecular weight of 13KD. The half-life of PCT is 25-30 hours, and the stability in vitro is very good. [0003] PCT is derived from a single-copy gene (the same gene as calcitonin gene-related peptide) located on chromosome 11 (11P15,4). The gene consists of 2800 base pairs, including 6 exons and 5 introns, and the total length of the gene is about 7.6Kb. After transcription, PCT mRNA is produced by specific splicing, and then translated into procalcitonin precur...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531G01N21/82
CPCG01N33/6812
Inventor 金鑫曾滨
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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