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Anti-Cyclic Citrullinated Peptide Antibody Assay Kit

A technology of anti-cyclic citrullinated peptide and kit, which is applied in the field of medical immunology in vitro diagnosis, can solve the problems of undetectable and high cost of measurement, and achieve the effects of simple operation, enhanced reaction absorbance, and improved automation

Active Publication Date: 2014-10-29
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two detection methods require solid phase or electrode support, and both require specific detection instruments, and cannot be directly detected on a fully automatic biochemical analyzer
[0004] In addition, the cost of single batch determination of samples by enzyme-linked immunosorbent assay is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0042] The components (percentage by weight) in the R1 reagent are:

[0043] PB buffer 0.4% Inorganic salt 1% TWEEN20 0.05% ProClin300 0.02% Interference scavenging protein (Roche ) 0.02% (Modified) CCP-sensitized nano-PS microspheres 0.1% Ultra-pure water 98.41%

[0044] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitized nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 60nm.

[0045] The components (percentage by weight) in the R2 reagent are:

[0046] PB buffer 0.4% Inorganic salt 1% TWEEN 20 0.05% ProClin300 0.02% Goat anti-human IgG-FC antibody 0.001% Ultra-pure water 98.529%

[0047] The above ingredients are mixed to obtain reagent 2.

[0048] When used, the mixing ratio of...

Embodiment 2

[0051] Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0052] The components (percentage by weight) in the R1 reagent are:

[0053] PB buffer 5% Inorganic salt 5% Emulgen120 1% Sodium azide 0.1% Interfering scavengers (scantibodies) 0.1% (Modified) CCP-sensitized nano-PS microspheres 0.06% Ultra-pure water 88.74%

[0054] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitized nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 200nm.

[0055] The components (percentage by weight) in the R2 reagent are:

[0056] PB buffer 5% Inorganic salt 5% EMULGEN120 1% Sodium azide 0.1% Goat anti-human IgG-FC antibody 0.3% Ultra-pure water 88.6%

[0057] The above ingredients are mixed to obtain reagent 2.

[0058] When used, the mixing ratio of the R1 r...

Embodiment 3

[0060]Anti-cyclic citrullinated peptide antibody assay kit, including R1 reagent and R2 reagent,

[0061] The components (percentage by weight) in the R1 reagent are:

[0062] Goods' buffer 10% Inorganic salt 10% TX100 2% Thimerosal 0.2% Interference clearing protein (Roche ) 0.2% (Modified) CCP-sensitized nano-PS microspheres 0.05% Ultra-pure water 77.55%

[0063] Mix the above ingredients to obtain Reagent 1; wherein, the (modified) CCP-sensitized nano PS microspheres can be prepared by chemical cross-linking method according to the prior art, and the diameter is 500nm.

[0064] The components (percentage by weight) in the R2 reagent are:

[0065] PB buffer 10% Inorganic salt 10% TX100 2% Thimerosal 0.2% Goat anti-human IgG-FC antibody 0.5% Ultra-pure water 77.3%

[0066] The above ingredients are mixed to obtain reagent 2.

[0067] When used, the mixing ratio of the R1 reagent...

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Abstract

The invention discloses a kit for determining an anti-cyclic citrullinated peptide (Anti-CCP) antibody. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, inorganic salt, surfactant, preservative, interference removing protein, modified CCP-sensitized nano poly styrene (PS) microspheres and ultrapure water; the reagent R2 comprises a buffer solution, inorganic salt, surfactant, preservative, an anti Anti-CCP second antibody and ultrapure water; and the weight ratio of the reagent R1 to the reagent R2 is (50-90):(10-50). According to the method, the Anti-CCP is determined by adding the anti Anti-CCP second antibody by a latex booster immunization transmission turbidimetry, a detection signal is subjected to two-stage amplification, the detection sensitivity is improved, and the determination range is enlarged; and the kit can be applied to an automatic biochemical analyzer to shorten detection time, and improve detection efficiency.

Description

Technical field [0001] The invention is a medical immunohic in vitro diagnostic field, involving an immune ratio detection agent. Furthermore, the invention involves anti -ring melonic acid peptide antibody detection kits. Background technique [0002] Anti-CCP is a polypeptide fragment of ring-shaped polyin, which is mainly an antibody.Clinical studies have shown that Anti-CCP has good sensitivity and specificity for rheumatoid arthritis (RA), and is related to RA's movement and course of disease. The detection of ANTI-CCP's early diagnosis and prognosis evaluation of RA is very good.Significant. [0003] At present, there are two methods for clinical testing Anti-CCP: 1. Enzyme-linked Immuno Sorbent Assay (ELISA); 2. ElectroChemiluminescence Immunoassay (ECLI).These two detection methods require solid phase or electrode support, both need specific detection instruments, and cannot directly automatically automatically biochemical analyzer detection. [0004] In addition, the cos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/82G01N21/31
Inventor 周方银韩帅谭韬周帅何涛涛
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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