Alpha1-AT (antitrypsin) immunoturbidimetry detection kit

An anti-trypsin and detection kit technology is applied in the field of α1-antitrypsin immunoturbidimetric detection kits, which can solve the problem of poor accuracy and stability, unfavorable clinical analysis and detection promotion and application, and α1-antitrypsin immunoturbidimetric detection kits. The problem of low analytical sensitivity of detection reagents is to achieve the effect of improving analytical sensitivity, solving mildew and enhancing stability.

Active Publication Date: 2015-02-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common α1-antitrypsin detection reagent has low sensitivity, poor accuracy and stab...

Method used

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  • Alpha1-AT (antitrypsin) immunoturbidimetry detection kit
  • Alpha1-AT (antitrypsin) immunoturbidimetry detection kit
  • Alpha1-AT (antitrypsin) immunoturbidimetry detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A conventional detection kit for α1-antitrypsin immunoturbidimetric method on the market, which includes reagent R1, reagent R2 and calibrator.

[0024] Wherein, the reagent R1 consists of:

[0025] pH 7.2 Phosphate buffer 50 mmol / L

[0026] Sodium chloride 0.1 mmol / L

[0027] Polyethylene glycol 400 40g / L

[0028] Reagent R2 consists of:

[0029] Goat anti-human α1-trypsin antibody 6ml / L

[0030] Sodium chloride 0.1 mmol / L

[0031] The α1-antitrypsin detection kit described in this embodiment, when in use, its assay method is to adopt Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0032] Add 8 μl of physiological saline, sample or calibrator, then add 200 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 80 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0033] The calibrator used in this example is the α1-AT calibrator produced by Ningbo Preble Biotechn...

Embodiment 2

[0035] A detection kit for α1-antitrypsin immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0036] Wherein, the reagent R1 consists of:

[0037] pH 7.2 Phosphate buffer 50 mmol / L

[0038] Sodium chloride 0.1 mmol / L

[0039] Polyethylene glycol 400 40g / L

[0040] Silica coated magnetic nanoparticles 0.1%

[0041] Reagent R2 consists of:

[0042] Goat anti-human α1-trypsin antibody 6ml / L

[0043] Sodium chloride 0.1 mmol / L

[0044] Bovine Serum Albumin (BSA) 20g / L

[0045] Proclin-300 1ml / L

[0046] Concrete determination method is with embodiment 1.

Embodiment 3

[0048]A detection kit for α1-antitrypsin immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0049] Wherein, the reagent R1 consists of:

[0050] pH 7.2 Phosphate buffer 50 mmol / L

[0051] Sodium chloride 0.1 mmol / L

[0052] Polyethylene glycol 400 40g / L

[0053] Silica coated magnetic nanoparticles 1%

[0054] Reagent R2 consists of:

[0055] Goat anti-human α1-trypsin antibody 6ml / L

[0056] Sodium chloride 0.1 mmol / L

[0057] Bovine Serum Albumin (BSA) 30g / L

[0058] Proclin-300 1ml / L

[0059] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a Alpha1-AT (antitrypsin) immunoturbidimetry detection kit and belongs to the technical field of clinical external detection reagents. The detection kit comprises a reagent R1, a reagent R2 and a calibrator. A certain amount of magnetic nano-particles covered with silicon dioxide are added in the reagent R1, a certain amount of BSA (bovine serum albumin) and Proclin-300 are added in the reagent R2, and accordingly analytical sensitivity and stability of the detection kit are improved, linear range thereof is expanded, reagents accuracy is higher, and further application and promotion in the market is facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an α1-antitrypsin immune turbidimetric detection kit. Background technique [0002] α1-antitrypsin (abbreviated as α1-AT or AAT) is a glycoprotein. Sugar 10% to 20%, mainly synthesized by the liver. It is the most important protease inhibitor in serum, and it also inhibits other enzymes such as thrombin and urokinase; it is also an acute phase reaction protein. In inflammatory diseases, α1-antitrypsin can enter through capillaries Interstitial fluid often has a high concentration in the local area of ​​inflammation, which has a certain limiting effect on acute inflammatory diseases. α1-antitrypsin is a non-specific soluble factor of the respiratory system, which is closely related to respiratory resistance. It can inhibit the activity of various enzymes, including bacterial enzymes, and proteases secreted by neutrophil lysosomes, elastase, Collagen...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 甘宜梧董雯谭柏清李静包兴艳朱玉娥肖慧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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