Biochemical analyzer and detection method thereof

Abstract

The invention relates to a biochemical analyzer and a detection method thereof. The biochemical analyzer comprises a base (12), a sampling module (4), an incubation device (3) and a detection device (6) are fixed on the base (12), the incubation device (3) consists of a linear guide rail 305 and an incubation tank 310, and the incubation tank 310 can move along the linear guide rail 305. The biochemical analyzer also includes a mobile device and a detection component, the mobile device enables the movement of a chip box (11) in the incubation tank 310 along the vertical direction of the linear guide rail 305, and the chip box (11) can be detected through the detection component. Through colorimetry and nephelometry dual detection, the analyzer provided by the invention can rapidly detect multiple items of one object or multiple items of multiple objects, and has the characteristics of single part, multiple targets, multiple channels, full automation, high flux and convenient use, etc.

Description

technical field [0001] The present application relates to a biochemical analyzer and a detection method thereof, in particular to a biochemical analyzer capable of simplifying structure and rapid detection and a detection method thereof. Background technique [0002] At present, there are many fully automatic biochemical analyzers, most of which adopt disc-type reaction disc, disc-type sample disc and reagent disc structure, such as the patent application whose publication number is CN104833812, which adopts disc-type reaction disc structure and reagent kits. A single item is packaged for multiple people; analysis samples are generally in batch mode, that is, multiple samples to be analyzed are all loaded on the sample tray, and after setting the corresponding program, the analyzer automatically performs batch analysis on the loaded samples. The structure, reagent packaging method and sample analysis method of such a biochemical analyzer are very suitable for the analysis of...

AI Technical Summary

Problems solved by technology

Since the biochemical reagents need to be calibrated and have a certain expiry date after opening the bottle, there are often cases where the reagents fail after opening the bottle, so that it is difficult for primary hospitals to carry out biochemical analysis.

[0004] In view of the fact that the current automatic biochemical analyzer is not suitable for the use of grassroots medical institutions, a fully automatic biochemical analyzer based on microfluidic chips and its supporting integrated chips have been developed, such as CN104833812, CN102580797 and other patents. The reagents use single servings and multiple targets The advanced packaging and detection methods can certainly meet the needs of primary hospitals for biochemical detection, but the system and detection methods have the following defects: 1. The chip uses freeze-dried reagents, reagent 1 (R1) and reagent 2 (R2) are put together, During the reaction, the sample reacts with R1 and R2 at the same time. Since there are many items of detection that require the sample to react with R1 first, and then add R2 to react after a period of time (such as the detection of homocysteine ​​based on the cyclic enzyme method, the detection of enzyme-based glycosylated hemoglobin), which limits the application of the instrument to the test items; 2. The instrument can only measure one chip at a time, and the detection time of each chip is about 10 minutes. This kind of detection throughput is very small and is only suitable for Basic medical institutions with a small amount of detection; 3. The chip structure is complex, and reagents need to be freeze-dried, resulting in relatively high cost of reagent chips; 4. Operators are required to manually add samples and diluents quantitatively, and the operator's operating level directly affects the measurement results the accuracy of

Immunoturbidimetry can be divided into transmission turbidimetry and scattering turbidimetry: transmission turbidimetry is easy to operate, but the sensitivity and precision are not ideal, the amount of antiserum required is large, and the detection period is long; scattering turbidimetry has high sensitivity and precision. High accuracy, fast detection

[0006] For the four cumbersome operations of adding samples, reagents, washing and interpreting the color reaction results in the detection, however, each stage itself has cumbersome steps, and at the same time, the structure of the steps in each stage of operation is also very complicated, resulting in the operation of the entire biochemical analyzer. Trouble and high cost. When judging the test results, the results are generally judged by colorimetry or turbidimetry. Among them, the turbidimetry method uses transmission turbidimetry, and it is rare to be able to perform both colorimetry and turbidimetry in one device. Scattering turbidimetric detection reduces the adaptability of the equipment. At the same time, the existing equipment must judge a large number of samples, and cannot detect both small batches and large batches. The detection instrument cannot meet the effect of rapid detection

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