Kit for detecting serum amyloid protein and application thereof

A serum starch and kit technology, applied in the field of immunodiagnosis, can solve the problems of difficulty in meeting market demands, low sensitivity and low specificity, and achieve the effects of shortening detection time, improving detection sensitivity and good anti-interference performance.

Inactive Publication Date: 2017-01-25
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there are only a few manufacturers of SAA kits approved by the China Food and Drug Administration (CFDA),

Method used

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  • Kit for detecting serum amyloid protein and application thereof
  • Kit for detecting serum amyloid protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Reagent R1 is composed of the following components: buffer (MES), inorganic salt (sodium chloride), surfactant (Triton X-100), preservative (Proclin 300), stabilizer (sucrose) and interference elimination protein ( HBR blocker).

[0046] Reagent R2 is composed of the following components: buffer (MES), inorganic salt (sodium chloride), surfactant (Triton X-100), preservative (Proclin 300), stabilizer (sucrose) and polystyrene latex Particle mixture, the polystyrene latex particle mixture is cross-linked with anti-serum amyloid SAA antibody, the latex preparation steps of coupled antibody include activation, coupling and mixing steps, the specific steps are as follows:

[0047] 1. In the coupling buffer (50mM MES, pH 6.0), wash 100mg of carboxylated latex particles with an average particle size of 82nm and 180nm respectively, and then carry out the antibody coupling reaction on the two kinds of latex according to the following steps, using 5mL Resuspend in coupling buff...

Embodiment 2

[0060] Reagent R1 consists of the following components: buffer (3-morpholine-2-hydroxypropanesulfonic acid), inorganic salt (potassium chloride), surfactant (polyoxyethylene laurate), preservative (Proclin 300), Stabilizer (metal complexing agent) and interference elimination protein (HBR blocker).

[0061] Reagent R2 consists of the following components: buffer (3-morpholine-2-hydroxypropanesulfonic acid), inorganic salt (potassium chloride), surfactant (polyoxyethylene laurate), preservative (Proclin 300), Stabilizer (metal complexing agent) and polystyrene latex particle mixture, the polystyrene latex particle mixture is cross-linked with anti-serum amyloid SAA antibody, the latex preparation steps of coupled antibody include activation, coupling, mixing steps ,Specific steps are as follows:

[0062] 1. In the coupling buffer (50mM, pH 6.0), respectively wash 100mg of carboxylated latex microparticles with an average particle size of 82nm and 180nm, and then carry out the ...

Embodiment 3

[0075] Reagent R1 consists of the following components: buffer (4-hydroxyethylpiperazineethanesulfonic acid), inorganic salt (magnesium sulfate), surfactant (polyoxyethylene oleate), preservative (thimerosal), stabilizer ( Antioxidants) and Interfering Elimination Proteins (HBR Blockers).

[0076] Reagent R2 consists of the following components: buffer (4-hydroxyethylpiperazineethanesulfonic acid), inorganic salt (magnesium sulfate), surfactant (polyoxyethylene oleate), preservative (thimerosal), stabilizer ( Antioxidant) and polystyrene latex particle mixture, described polystyrene latex particle mixture is cross-linked with anti-serum amyloid SAA antibody, the latex preparation step of coupling antibody comprises activation, coupling, mixing steps, concrete steps are as follows:

[0077] 1. In the coupling buffer (50mM, pH 6.0), respectively wash 100mg of carboxylated latex particles with an average particle size of 82nm and 500nm, and then carry out the coupling antibody re...

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Abstract

The invention provides a kit for detecting serum amyloid protein and application thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein; the reagent R2 is prepared from the buffer solution, the inorganic salt, the surfactant, the preservative, the stabilizer and a polystyrene latex particle mixture; the polystyrene latex particle mixture is cross-linked with an SAA (Serum Amyloid A) antibody; the polystyrene latex particle mixture is a mixture of large-diameter polystyrene latex particles and small-diameter polystyrene latex particles. The kit disclosed by the invention is based on PETIA (Particle-enhanced Turbidimetric Immunoassay) and can be generally used for analysis of all kinds of full-automatic biochemical analyzer; during use, the required determining time is short, the specificity is high, the precision degree is high, and the accuracy degree is high.

Description

technical field [0001] The invention belongs to the field of immunodiagnosis, and in particular relates to a kit for detecting serum amyloid and its application. Background technique [0002] Serum amyloid A (Serum amyloid A, SAA) is an acute phase response protein, which belongs to the heterogeneous protein in the apolipoprotein family, with a relative molecular mass of about 12,000. In the acute phase reaction, stimulated by interleukin (Interleukin, IL) and tumor necrosis factor (Tumor necrosis factors, TNF), SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100 to 1000 times the initial concentration. Similar to C-reactive protein (CRP), SAA is a sensitive indicator reflecting the early inflammation of infectious diseases, which is helpful for diagnosing inflammation, evaluating its activity, monitoring its activity and treatment. [0003] At present, many methods have been applied to the detection of serum SAA, including r...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 华权高来祥兵徐春雷
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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