Microfluidic chip for scattering turbidimetry determinationand biochemical immunization machine using same

Abstract

The invention provides a microfluidic chip for scattering turbidimetry determinationand a biochemical immunization machine using same, which belong to the field of determination equipment. The microfluidic chip for scattering turbidimetry determination comprises a body, a mixing slot, and reaction holes, the body is provided with a sample adding slot, a sample quantification slot, and a sample overflow slot, the sample adding slot is communicated with the sample quantification slot, the sample quantification slot is communicated with the sample overflow slot, the body is also provided with a diluent adding slot, a diluent quantification slot, and a diluent overflow slot, the diluent adding slot is communicated with the diluent quantification slot, the diluent quantification slot is communicated with the diluent overflow slot, both the sample quantification slot and the diluent quantification slot are communicated with the mixing slot, the mixing slot is communicated with the reaction holes through a flow passage assembly, one side, which is close to the circle center of the body, of each reaction hole is planar, and the parts, which correspond to the outer circles of the reaction holes, of the body are convex arcs. According to the invention, the scattering of scattering turbidimetry determination light can be effectively controlled, determination light intensity sensitivity isimproved, and the invention can be used in colorimetry determination and transmission turbidimetry assay.

Description

technical field [0001] The invention belongs to the field of detection equipment, and relates to a biochemical and immune detection device, in particular to a scattering turbidimetric detection microfluidic chip and a biochemical immune machine using the chip. Background technique [0002] Microfluidic chip technology is a new technology for precisely manipulating and controlling nanoliter and picoliter-level fluids (biological sample fluids) in micron-scale flow channels. The application of this technology can integrate sample preparation, The basic operation units such as reaction, separation, detection, cell culture, sorting, and lysis are integrated or basically integrated on a chip of several square centimeters (or even smaller), and a network of microfluidic channels is formed to control the flow of fluid throughout the system. A technology platform used to replace the various functions of conventional chemical or biological laboratories. The basic feature and biggest ...

AI Technical Summary

Problems solved by technology

[0005] At present, there are biochemical detection chips based on microfluidic technology on the market. This chip uses microfluidic chip technology to integrate various cuvettes for biochemical reactions into the reagent reaction wells around the circular disc, which can realize the utilization of specific cuvettes. The biochemical detection items and immunological detection items detected by color method and turbidimetric method, but when the chip is detected by scattering turbidimetry, because the reagent reaction hole of the chip is cylindrical, the inner wall of the detection hole and the outer wall of the chip A structure similar to a plano-concave lens is formed, which has a divergent effect on the scattered light, causing the detector to detect a part of the light intensity when it detects the scattered light intensity. For the detection of scattered light intensity with lower energy The sensitivity is not enough, it will affect the performance index of the whole detection system

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