The embodiment of the invention provides a positioning method, device and system, and relates to the technical field of electronics. The accuracy and efficiency of wiping a printing plate are improved. The scheme includes the steps that if a substrate comprises a bad point position, the positioning device obtains first coordinate information, in a substrate coordinate system, of the bad point position, and the first coordinate information is used for indicating the position, on the substrate, of the bad point position; according to the preset coordinate corresponding relation between the substrate coordinate system and a printing plate coordinate system, the positioning device converts the first coordinate information into second coordinate information in the printing plate coordinate system, and the second coordinate information is used for indicating the position, corresponding to the bad point position on the substrate, of the printing plate; the positioning device outputs a position signal, corresponding to the second coordinate information, on the printing plate. The scheme can be used for positioning the bad point position on the printing plate.