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60results about How to "Convenient and accurate determination" patented technology

Method for establishing high-throughput sequencing library and application thereof

The invention discloses a method for establishing a high-throughput sequencing library and an application thereof. The method for establishing the high-throughput sequencing library comprises the following steps of: conducting DNA (Deoxyribose Nucleic Acid) fragmentation on a genome; conducting end repairing on DNA fragments; adding a basic group A to a 3' end of the DNA ends subjected to end repairing; connecting the DNA fragments with sticky ends A with methylated joints; conducting hybridization capture on the connection products by using a specific probe so as to obtain target fragments; conducting bisulfite treatment on the target fragments so as to convert non-methylated cytosine in the target fragments into uracil; conducting PCR (Polymerase Chain Reaction) amplification on the converted target fragments; separating and purifying the amplification products; and establishing the high-throughput sequencing library by using the amplification products. By utilizing the method for establishing the high-throughput sequencing library and the application thereof, the high-throughput sequencing library of a specific genome area of a sample can be conveniently and effectively established.
Owner:BGI TECH SOLUTIONS

Tracking method of circuit board quality

The invention discloses a method for tracing quality of a circuit board. The method comprises the following steps of: providing a circuit board substrate, wherein the circuit board substrate comprises a plurality of product areas and a non-product area, and a first identification code is formed in the non-product area of each circuit board substrate; forming a conductive circuit in the plurality of product areas of the circuit board substrate; laminating a plurality of the circuit board substrates which are used for manufacturing a same multilayer circuit board substrate to obtain a multilayer circuit board substrate, wherein all the product areas which correspond to one another form a circuit board unit; and forming a second identification code on the surface of the multilayer circuit board substrate, wherein the second identification code is correlative to the first identification codes of the plurality of the circuit board substrates which are used for manufacturing the same multilayer circuit board substrate; forming a third identification code on the surface of each circuit board unit, wherein the circuit board unit of the circuit board unit which belongs to the same multilayer circuit board substrate is mutually correlative to the corresponding second identification code; and shaping the multilayer circuit board substrate to obtain a plurality of circuit board units which are separated from one another.
Owner:江门市东辉电子科技有限公司

Pavement roughness detection system and method

The invention relates to a pavement roughness detection system and method, wherein the system comprises at least one reference target, arranged above a to-be-measured pavement; at least one line structured light sensor, used for casting line structured light to the to-be-measured pavement and shooting a light bar image of the line structured light, formed on the to-be-measured pavement; and a computing device, connected with the line structured light sensor; the computing device converts the light bar image received from the line structured light sensor into three-dimensional data, according to the reference target, the three-dimensional data of the to-be-measured pavement is modified, and according to the modified three-dimensional data, the roughness of the to-be-measured pavement is determined. By use of the line structured light sensor for capturing the light bar image of the to-be-measured pavement, then the light bar image is converted into the three-dimensional data, according to the reference target, the three-dimensional data of the to-be-measured pavement is modified, and the roughness of the to-be-measured pavement can be conveniently and accurately determined.
Owner:RES INST OF HIGHWAY MINIST OF TRANSPORT

Focal position determining apparatus, method and device for laser processing

The invention provides a focal position determining apparatus, method and device for laser processing and relates to the technical field of laser processing. The focal position determining apparatus for laser processing comprises a laser processing head with an annular wall, a focusing lens, a CCD coaxial field-of-view monitor and multipath laser instruction generators; the focusing lens is arranged in the laser processing head with the annular wall; the multipath laser instruction generators are arranged on the periphery of the annular wall so as to be used for emitting multiple paths of laser instruction light to shine the surface of a workpiece; the multiple paths of laser instruction light can be overlapped in a laser processing focal position; the CCD coaxial field-of-view monitor is arranged at the upper end of the laser processing head with the annular wall so as to monitor the overlapping situation information of the multiple paths of laser instruction light on the surface of the workpiece; and the focal position of laser processing is determined through the overlapping situation information. By means of the focal position determining apparatus, method and device for laser processing, the problems of easily and efficiently determining the laser focal position in the laser processing process and improving the quality and efficiency of laser process can be solved.
Owner:AVIC BEIJING AERONAUTICAL MFG TECH RES INST

Positioning method, device and system

The embodiment of the invention provides a positioning method, device and system, and relates to the technical field of electronics. The accuracy and efficiency of wiping a printing plate are improved. The scheme includes the steps that if a substrate comprises a bad point position, the positioning device obtains first coordinate information, in a substrate coordinate system, of the bad point position, and the first coordinate information is used for indicating the position, on the substrate, of the bad point position; according to the preset coordinate corresponding relation between the substrate coordinate system and a printing plate coordinate system, the positioning device converts the first coordinate information into second coordinate information in the printing plate coordinate system, and the second coordinate information is used for indicating the position, corresponding to the bad point position on the substrate, of the printing plate; the positioning device outputs a position signal, corresponding to the second coordinate information, on the printing plate. The scheme can be used for positioning the bad point position on the printing plate.
Owner:HEFEI BOE OPTOELECTRONICS TECH +1

Testing device and method for effective compaction time of asphalt pavement

The invention discloses a testing device for the effective compaction time of an asphalt pavement. The testing device comprises a temperature sensor, a data acquisition instrument and a stopwatch as well as a bracket, wherein the temperature sensor and the data acquisition instrument are connected through a lead, the bracket comprises a pointed-end rod and a slider, the slider is arranged on the pointed-end rod in a sheathed manner and provided with a fixed circular hoop, and the temperature sensor is inserted into the fixed circular hoop. Through the temperature sensor and the data acquisition instrument, the average temperature of the asphalt pavement at the to-be-tested position and the change law of the average temperature can be conveniently and accurately tested; by controlling the effective compaction time of paving layers of the asphalt pavement in the rolling process, scientific rolling can be conducted, the operation with manual experience only is avoided, and the requirements on the compactness and practicability of the asphalt pavement can be met to the maximum extent.
Owner:SOUTHEAST UNIV

Nondestructive testing method and device for crack of metal part

The invention relates to a nondestructive testing method and a nondestructive testing device for a crack of a metal part. The nondestructive testing method for the crack of the metal part comprises the following steps of (1), connecting the two ends of a to-be-tested part with a direct-current power supply, and enabling an infrared lens of an infrared thermal imager to face the to-be-tested part; (2), selecting the time and the size that the to-be-tested part is subjected to the direct-current power according to the dimension and the material property of the to-be-tested part and a position where the crack damage exists, and electrifying the to-be-tested part; (3), after the electrification of the to-be-tested part is completed, observing and imaging the electrified to-be-tested part through the infrared thermal imager, showing that the crack does not exist in the to-be-tested part if an imaged image is in a same color, and showing that the crack exists in the to-be-tested part if the imaged image is in different colors. As a circumferential motion effect and a Joule heating effect are adopted for the nondestructive testing method and the nondestructive testing device for the crack of the metal part, higher temperature rises can be automatically generated at the position of a crack tip by both the circumferential motion effect and the Joule heating effect, and the position of the crack damage of the part is easily and accurately determined. In view of reasons above, the nondestructive testing method and the nondestructive testing device for the crack of the metal part can be widely used for the field of the nondestructive testing of metal cracks.
Owner:DALIAN UNIV OF TECH

Method and system of reducing hierarchical access direct current commutation failure risk

PendingCN107069793AReasonably arrange spare capacityReduce the risk of layered access DC commutation failureElectric power transfer ac networkAc network voltage adjustmentTime domainWeight coefficient
The invention relates to a method and system of reducing a hierarchical access direct current commutation failure risk. The method of reducing a hierarchical access direct current commutation failure risk includes the steps: determining a key fault set influencing a hierarchical access direct current inversion side converter station; adjusting reactive power output of dynamic reactive compensation equipment, performing time domain simulation on the key fault set, and calculating the reactive voltage control sensitivity of a dynamic reactive source; sorting m reactive voltage control flexibilities, and calculating the weight coefficient of the dynamic reactive source; and after calculating the reactive standby capacity of the dynamic reactive source based on the weight coefficient, establishing a dynamic reactive standby optimization model, and utilizing an inner point method to solve the dynamic reactive standby optimization model to obtain the optimized reactive standby capacity. The method and system of reducing a hierarchical access direct current commutation failure risk can provide an optimization model for reducing the hierarchical access direct current commutation failure risk, can improve the transmission capability of the alternating and direct current power transmission channel, and has significant meaning for improving operation stability of power grid.
Owner:CHINA ELECTRIC POWER RES INST +1

Program play control method and device

InactiveCN106375811AImprove usabilityRealize the technical effect of playback controlSelective content distributionElectricityComputer engineering
The invention discloses a program play control method and device, relates to the technical field of a frontend application of an internet television. The technical problem that in the prior art, a user cannot control program play under the condition that a remote controller cannot be found or a battery of the remote controller is powered off due to the fact that the dependence on the remote controller is high when the program play is controlled is solved. The method comprises the steps of in the process of playing a video program, identifying a gesture feature when the user waves a gesture; determining corresponding play control information according to the identified gesture feature; and controlling the video program according to the play control information, wherein the control comprises play progress control and voice adjustment control.
Owner:TVMINING BEIJING MEDIA TECH

Method and device for determining flying shooting trigger point

The invention provides a method and a device for determining a flying shooting trigger point. The method comprises the following steps: S1, obtaining a first image at a shooting point through static shooting; s2, intercepting a sub-image in the first image; s3, in the flying shooting process, sending a trigger signal to the camera at an undetermined shooting trigger point corresponding to the shooting point to obtain a second image; s4, performing template matching in the second image by using the sub-image as a template to obtain a target object in the second image; s5, obtaining coordinate information of the sub-image in the first image and coordinate information of the target object in the second image, and calculating a position deviation between the target object in the second image and the sub-image in the first image; and S6, adjusting the to-be-determined shooting trigger point according to the position deviation to obtain an actual shooting trigger point. According to the method, the deviation between the flying image and the actually required image can be conveniently, effectively and quantitatively determined, so that the shooting trigger point of the camera in the flying shooting process can be conveniently and accurately determined.
Owner:CHANGZHOU MICROINTELLIGENCE CO LTD

Antenna lodging control device based on CAN bus

The invention belongs to the technical field of antenna control and discloses an antenna lodging control device based on a CAN bus. The antenna lodging control device comprises a master control circuit, an isolation CAN communication circuit, an angle detection circuit, a motor drive circuit and a motor. The isolation CAN communication circuit has a data interface for connecting with an external device; the isolation CAN communication circuit, the angle detection circuit and the motor drive circuit are connected with the master control circuit; and the driving end of the motor is connected with the motor drive circuit. The isolation CAN communication circuit receives an external angle control instruction, and the motor drive circuit is controlled by the master control circuit to drive themotor to rotate to realize antenna lodging; and lodging angle of the antenna is detected in real time through the angle detection circuit, and the master control circuit controls the motor drive circuit according to the lodging angle detection value to drive the motor to adjust rotation so as to realize accurate adjustment of the antenna lodging angle. The antenna lodging control device based on the CAN bus has a communication control function and an angle control feedback function.
Owner:武汉中电通信有限责任公司

Body position icon, adding method, control device and ultrasonic equipment of control device

The invention provides a body position icon. The body position icon is located at the position, used for identifying ultrasonic examination, of an ultrasonic display interface and is a three-dimensional body position icon; the invention further provides a control device and equipment for the body position icon. By means of the body position icon, different positions of the three-dimensional body position icon can be marked according to a probe, thereby an operator can determine the examination position conveniently and accurately, operation convenience of doctors is improved, and meanwhile the aesthetic effect is enhanced.
Owner:SONOSCAPE MEDICAL CORP

Image processing method and electronic device

ActiveCN107346544AAvoid adverse effects of beautification effectsAchieving a balanced level of skin responseImage enhancementImage analysisPattern recognitionImaging processing
The invention provides an image processing method and an electronic device. The image processing method comprises the steps of detecting a face object in an image; based on the detected face object, respectively acquiring a first skin response bitmap of the left side face of the face object and a second skin response bitmap of the right side face; correspondingly comparing the skin response values of each pixel of the first skin response bitmap and the second skin response bitmap, and on the condition that the difference between the skin response value of a first pixel in the first skin response bitmap and the skin response value of the first pixel in the second skin response bitmap is larger than a preset value, adjusting the skin response value of the first pixel in the first skin response bitmap and the skin response value of the first pixel in the second skin response bitmap to the skin response value which is relatively high in the two skin response values, wherein the first pixel in the first skin response bitmap and the first pixel in the second skin response bitmap are pixels at corresponding area positions. The image processing method and the electronic device overcome adverse influences of factors such as illumination to face beautifying.
Owner:LENOVO (BEIJING) LTD

Electric rotary clamp for rock cutting machine

The invention relates to the technical field of machinery, and particularly relates to an electric rotary clamp for a rock cutting machine. The electric rotary clamp comprises a guide rail, a special-shaped frame, a roller center frame, the rock cutting machine and a four-claw self-centering chuck, wherein the special-shaped frame and the roller center frame are mounted on the guide rail through linear bearing sliding blocks, the rock cutting machine is mounted between the special-shaped frame and the roller center frame, and the four-claw self-centering chuck is mounted on the special-shapedframe. The tail end of the four-claw self-centering chuck is connected with the driving end of a motor horizontally embedded in the special-shaped frame through a bearing, and the motor drives the four-claw self-centering chuck to rotate around the axis to drive a rock sample to rotate around the axis; and the four-claw self-centering chuck and the roller center frame firmly fix the two ends of the cylindrical rock sample, the cylindrical rock sample is horizontally arranged above a cutting table of the rock cutting machine, and a circular blade of the rock cutting machine is perpendicular tothe cylindrical rock sample. According to the electric rotary clamp, the cylindrical sample can continuously rotate around the axis in the rock cutting process, and the cutting position can be quicklyand accurately determined.
Owner:TIANJIN UNIV

Method and apparatus for determining a signal offset of a pitch rate sensor

In order to determine a signal offset (OFF_SIG) of a pitch rate sensor of a vehicle (2), a signal (OMEGA_TETA_SIG) from the pitch rate sensor is detected. Longitudinal acceleration (ACC) of the vehicle (2) is determined. A time derivative (ACC_DRV) of the longitudinal acceleration (ACC) is determined. A check is carried out in order to determine whether a magnitude of the time derivative (ACC_DRV) of the longitudinal acceleration (ACC) is less than a first predefined threshold value (THD_1). The signal offset (OFF_SIG) of the pitch rate sensor is determined on the basis of the signal (OMEGA_TETA_SIG) from the pitch rate sensor if the magnitude of the time derivative (ACC_DRV) of the longitudinal acceleration (ACC) is less than the first predefined threshold value (THD_1).
Owner:CONTINENTAL AUTOMOTIVE GMBH

Analogy method and system of high-rise structure applying viscous damper

ActiveCN105373639AReduce mistakesMeet the requirements of engineering precisionSpecial data processing applicationsSimulation basedEngineering
The invention discloses an analogy method and a system of a high-rise structure applying a viscous damper. The analogy method comprises the steps of establishing a standard three-dimensional solid model, wherein the standard three-dimensional solid model comprises a spring K1, a spring K2 and a spring K3*; the spring K2 and the spring K3* are connected in series and then connected with the spring K1 in parallel to co-support mass points; the spring K* is equivalently converted into the viscous damper; the standard three-dimensional solid model is converted to obtain an analogy structural model with the viscous damper; calculating in a complex number space based on the analogy structural model with the viscous damper to obtain an additional damping ratio algorithm; performing derivation and minimum value calculation on the reciprocal of the additional damping ratio algorithm to obtain a maximum additional damping ratio algorithm; and calculating through the maximum additional damping ratio algorithm to obtain the maximum damping ratio. In addition, the invention also provides an analogy system of the high-rise structure applying the viscous damper. By adoption of the scheme, the error can be obviously reduced and the engineering precision demand can be satisfied without actually applying the viscous damper in the high-rise building structure for pilot tests.
Owner:深圳华森建筑与工程设计顾问有限公司

Time synchronization method and device and electronic equipment

The invention provides a time synchronization method and device and electronic equipment, and the method comprises the steps: initiating a synchronization request to a classroom server, and determining the local initiation time; receiving a response message returned by the classroom server, and determining local receiving time, wherein the response message comprises server response time; determining a time error between the local client and the classroom server according to the local initiating time, the local receiving time and the server response time, and determining target time according to the time error; and executing a corresponding operation according to the target time. Through the time synchronization method and device and the electronic equipment provided by the embodiment of the invention, the time error between the local client and the classroom server can be conveniently and accurately determined under the condition of not paying attention to the specific size of the network delay; and thus, the local client can determine the target time matched with the server time of the classroom server. The method is simple in calculation and high in accuracy.
Owner:北京拓课网络科技有限公司

Optical fiber detection device for monitoring flowing direction and flowing speed of underground water in observation well

ActiveCN111638384ASolve the problem that only basic water quality information can be detectedSolve the disadvantage of being unable to judge the direction of water flowIndication/recording movementFluid speed measurementEngineeringElectromagnetic environment
The invention discloses an optical fiber detection device for monitoring the flowing direction and the flowing speed of underground water in an observation well. The device comprises a device shell (1); an electronic compass (3) is movably arranged in the device shell (1); the lower part of the electronic compass (3) is connected with a connecting pipe (7); the lower part of the connecting pipe (7) is connected with a steering fan (6) in a vertical direction; the center of the electronic compass (3) is hollow, a steel pipe (8) penetrates through the center of the electronic compass (3) and isfixedly connected with the device shell (1) and used for fixing an optical fiber (2), the steel pipe (8) and the optical fiber (2) penetrate through the connecting pipe (7), and the lower end of the device shell (1) is connected with an optical fiber support (10) used for fixing a lower endpoint of the optical fiber (2). The optical fiber detection device for monitoring the flow direction and theflow speed of the underground water in the observation well is low in cost, not prone to being interfered by the surrounding electromagnetic environment and more accurate and stable by being comparedwith a common probe.
Owner:HOHAI UNIV

Apparatus and method for monitoring temperature of cable joint of cable connected to gas insulated switchgear

An apparatus and a method for monitoring a temperature of a cable joint (201) of a cable (200) connected to a gas insulated switchgear (300). The apparatus comprises an adaptor (101) adapted to be coupled to a cable socket (31) of the switchgear, the cable socket being provided with a mounting hole (311) for connecting the cable joint, the mounting hole being provided at a mounting surface (312),wherein the adaptor, when coupled to the cable socket, is situated at the mounting surface and outside the mounting hole; a temperature sensor (102) arranged in the adaptor and configured to detect atemperature of the adaptor; and a processor (103) configured to determine the temperature of the cable joint based on the detected temperature of the adaptor. Then the temperature of the cable joint may be determined conveniently and precisely.
Owner:ABB (SCHWEIZ) AG

Automatic lamp searching method of intelligent evacuation system

The invention provides an automatic lamp searching method of an intelligent evacuation system and aims at accurately, simply and conveniently searching intelligent evacuation emergency illumination lamps and evacuation indicating lamps in the system and reflecting the specific installing positions of the intelligent evacuation emergency illumination lamps and the evacuation indicating lamps in passageways in the intelligent evacuation system. The automatic lamp searching method comprises the following steps that intelligent indicating lamps are cured with unique physical codes, and the surfaces of lamp bodies are provided with bar codes, two-dimensional codes or physical node digits corresponding to the physical codes. When the system operates for the first time, the mainframe automatically searches all the intelligent evacuation emergency illumination lamps and evacuation indicating lamps subordinate to the system, all the intelligent indicating lamps upwards feed back their physical code information, a mobile terminal performs interactive communication with the mainframe through a mobile network, provides a code scanning and identifying function or / and a manual inputting function and calls out corresponding setting interfaces of the intelligent evacuation emergency illumination lamps and the evacuation indicating lamps according to identified or used-input physical codes, input items of the installing positions are provided on the setting interfaces, and setting information containing the physical codes and the installing positions is submitted to the mainframe after lamp settings and installing position entering are completed.
Owner:广东东君照明有限公司

Method and system for configuring remote control function data according to image of remote control equipment

The embodiment of the invention provides a method and system for configuring remote control function data according to an image of remote control equipment. The method comprises the steps of obtaininga first image of the remote control equipment, and determining a first feature based on the first image, wherein the first feature comprises a key layout feature of the remote control equipment; anddetermining remote control function data corresponding to the remote control equipment based on the first feature. By identifying the image of the remote control equipment and determining the corresponding remote control function data according to the key feature layout, the remote control function data can be configured more conveniently and accurately, and the user experience is improved.
Owner:HANSONG NANJING TECH LTD

Method for establishing high-throughput sequencing library and application thereof

The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic DNA; end-repairing the DNA fragments; adding a base A to the 3′ end of the end-repaired DNA fragments; connecting the DNA fragments having cohesive end A with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; PCR amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample.
Owner:BGI TECH SOLUTIONS

Device and method for detecting sealing performance of cross-layer drilling extraction blocking section

The embodiment of the invention discloses a device and a method for detecting the sealing performance of a cross-layer drilling extraction blocking section, and relates to the technical field of coal bed gas drilling extraction. The device comprises a three-way pipe fitting, wherein the three-way pipe fitting is provided with a main pipe and a branch pipe connected to one side of the main pipe, an internal channel of the branch pipe communicates with an internal channel of the main pipe, and a pressure monitoring element is arranged on the branch pipe; and an upper opening of the main pipe is connected with a first sealing joint, the first sealing joint is used for being connected with an extraction pipe, a lower opening of the main pipe is connected with a second sealing joint, a liquid return pipe is inserted into the main pipe in a penetrating mode, the lower end of the liquid return pipe penetrates through the second sealing joint to be exposed, the second sealing joint enables the liquid return pipe to be in sealed connection with the lower opening of the main pipe, the upper end of the liquid return pipe penetrates through the first sealing joint to be sleeved with the extraction pipe, and annular spaces with certain gaps are formed between the inner wall of the first sealing joint and the outer wall of the liquid return pipe and between the inner wall of the extraction pipe and the outer wall of the liquid return pipe respectively. According to the device and the method, the sealing performance of the drilling blocking section can be conveniently and rapidly detected, and the position of a leakage point can be accurately detected.
Owner:HENAN POLYTECHNIC UNIV

Simulation method and system for high-rise structures with viscous dampers

ActiveCN105373639BReduce mistakesMeet the requirements of engineering precisionSpecial data processing applicationsDamping ratioEngineering
The invention discloses an analogy method and a system of a high-rise structure applying a viscous damper. The analogy method comprises the steps of establishing a standard three-dimensional solid model, wherein the standard three-dimensional solid model comprises a spring K1, a spring K2 and a spring K3*; the spring K2 and the spring K3* are connected in series and then connected with the spring K1 in parallel to co-support mass points; the spring K* is equivalently converted into the viscous damper; the standard three-dimensional solid model is converted to obtain an analogy structural model with the viscous damper; calculating in a complex number space based on the analogy structural model with the viscous damper to obtain an additional damping ratio algorithm; performing derivation and minimum value calculation on the reciprocal of the additional damping ratio algorithm to obtain a maximum additional damping ratio algorithm; and calculating through the maximum additional damping ratio algorithm to obtain the maximum damping ratio. In addition, the invention also provides an analogy system of the high-rise structure applying the viscous damper. By adoption of the scheme, the error can be obviously reduced and the engineering precision demand can be satisfied without actually applying the viscous damper in the high-rise building structure for pilot tests.
Owner:深圳华森建筑与工程设计顾问有限公司

Cleaning object material identification method, washing machine and storage medium

The embodiment of the invention discloses a method for identifying the material of a cleaned object, a washing machine and a storage medium. The method for identifying the material of the cleaning object comprises the following steps: acquiring the weight of a loaded cleaning object, and a preset water level and preset stirring time corresponding to the weight of the loaded cleaning object; detecting whether the water level reaches a preset water level, if the water level reaches the preset water level, stirring the load cleaning object according to preset stirring time, and obtaining a water level frequency change value corresponding to water level change; based on the weight of the load cleaning object, the water level frequency change value and the preset stirring time, the water absorption rate of the load cleaning object is calculated; and determining the material of the load cleaning object according to the weight of the load cleaning object and the water absorption rate of the load cleaning object. According to the identification method, the material type of the loaded cleaning object can be conveniently and accurately determined, and the relevant parameters in the cleaning process are subsequently adjusted according to the material type of the loaded cleaning object, so that the cleaning object is cleaned more cleanly.
Owner:HISENSE(SHANDONG)REFRIGERATOR CO LTD
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