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Momordica grosvenori extractive with at least 40 percent grosvenor momordica glycoside and preparation method thereof

A technology of Luo Han Guo extract and mogroside, which is applied in the field of active ingredients of Luo Han Guo and its extraction, can solve the problems of low content of extract products, no application of industrial production, and being in the laboratory stage, so as to shorten the production cycle and reduce the cost of processing. The amount of material, the effect of improving color

Inactive Publication Date: 2009-12-23
GUILIN HUITONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many extraction methods for mogroside V, but most of them are in the laboratory stage and have not been applied to industrial production.
In addition, the content of mogroside V extract products is not high. There are only two specifications on the market, one is 80% mogroside and 25% mogroside, the other is 95% mogroside and 30% mogroside. See the report that there are monk fruit extracts with mogroside ≥ 98% and mogroside V ≥ 40% as products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing a high-content Luo Han Guo extract with mogroside ≥ 98%, mogroside ≥ 40%, and a milky white color, comprising the following steps:

[0026] 1. Extraction: crush 1000Kg of fresh Luo Han Guo and put it into the extraction tank (it is better not to break the seeds), extract 3 times with purified water in a slightly boiling state, add 4000Kg of purified water each time, and the extraction time is 1.5 h, 1h, 1h, the filter residue after each filtration enters the next extraction;

[0027] 2. Concentration: combine the 3 times of Luo Han Guo extracts and concentrate to fresh fruit: the concentrated solution is 1:4, that is, 4000L;

[0028] 3. Enzymolysis: Cool the concentrated solution to 45-50°C, then turn on the steam to keep the temperature within this range, add citric acid to adjust the pH value to 3.4, then add 0.3Kg of pectinase with 0.03% of fresh fruit weight, in Enzymolysis at this temperature for 1 hour; heat up to 90°C, inactivate pectinase...

Embodiment 2

[0034] Implement in the mode of embodiment 1, difference is:

[0035] 1. Enzymatic hydrolysis, adding citric acid to adjust the pH value to 3.4, adding 0.05% pectinase by weight of fresh fruit, hydrolyzing for 60 minutes, inactivating at 90°C, adding protease by adding 0.05% by weight of fresh fruit, hydrolyzing for 40 minutes;

[0036] 2. Suction filter and refine with AB-8 macroporous adsorption resin, desorb with 60°C ethanol, and collect 2400L;

[0037] 3. Decolorize with LSA-700 strong base ion exchange resin column, and desorb with ethanol at 50 degrees;

[0038] 4. Recover ethanol and concentrate it to 17 degrees Baume;

[0039] 5. Spray drying to obtain 6.5Kg of milk white finished product.

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PUM

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Abstract

The invention provides a milk white fructus momordicae extract, fructus momordicae glycoside V and fructus momordicae glycoside of which are respectively larger than or equal to 40 percent and 98 percent. The invention also discloses a preparation method of the extract. Using fresh fructus momordicae as the raw material, the invention is obtained through the water extraction, the concentration, the enzymatic hydrolysis, the macroporous adsorption resin refining, the ion exchange resin decoloration, the concentration and the spray drying. The invention is characterized in that the process parameters of the steps which are suitable for the industrial production are found, the yield and the quality of the fructus momordicae extraction are all obviously improved, and obvious economic benefits and social benefits are obtained.

Description

technical field [0001] The invention relates to active ingredients of Luo Han Guo and an extraction method thereof, in particular to Luo Han Guo extract with mogroside V content ≥ 40% and a preparation method thereof. Background technique [0002] The main active ingredient in the Luo Han Guo extract is mogroside, and Mogroside V (Mogroside V) is the main sweet component in the mogroside compounds, and it is also a test substance when the content of the Luo Han Guo extract is determined. It not only has the characteristics of high sweetness, low calorie and good taste, but also has various medicinal and health functions. At present, there are many extraction methods for mogroside V, but most of them are in the laboratory stage and have not been applied to industrial production. In addition, the content of mogroside V extract products is not high. There are only two specifications on the market, one is 80% mogroside and 25% mogroside, the other is 95% mogroside and 30% mogro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00A23L1/221A61K36/42A23L27/10
Inventor 刘庚贵
Owner GUILIN HUITONG BIOTECH
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