Surgical smoke biological toxicity evaluation method

Abstract

The invention discloses a surgical smoke biological toxicity evaluation method. The method comprises the following steps: A, evaluating the in-vitro biological toxicity of luminescent bacteria; B, evaluating the in-vivo biological toxicity of ApoE gene-deficient mice exposed to surgical smoke; and C, evaluating the genetic toxicity of surgical smoke through micronucleus test. The method of the invention has the advantages of quickness and efficiency, accuracy and quantification, realness and reliability, multi-level evaluation, and the like, and has very high practical value and a broad market prospect. The method gets away from the embarrassing situation in which the biological toxicity of surgical smoke cannot be tested fully, and is expected to become an important way of evaluation and test for controlling surgical smoke pollution.

Description

technical field [0001] The invention relates to a biological toxicity evaluation method of surgical smoke. Background technique [0002] With the continuous development of minimally invasive surgery in modern medicine, the surgical smoke generated during the operation is very harmful to health and can cause acute and chronic poisoning of the human body. Surgical smoke can directly cause headaches for medical staff and patients in the operating room , eye nose and throat pain and inflammation and other symptoms. Pathogenic bacteria may also spread through the spread of surgical smoke, leading to infection of medical staff in the operating room. Surgical smoke can also produce genotoxicity. A large number of studies have proved that surgical smoke is potentially mutagenic and carcinogenic, and can produce genotoxicity. However, over the past few years, due to the limitation of conditions and the level of cognition, there are very few studies on surgical smoke, especially in ...

AI Technical Summary

Problems solved by technology

However, over the past few years, due to the limitation of conditions and the level of cognition, there are very few studies on surgical smoke, especially in China, which reduces the way people know

In addition, because surgical smoke generally does not cause acute and chronic poisoning with obvious symptoms, it is easy to be ignored by people.

However, due to long-term exposure of medical personnel in the operating room to substances including: incompletely burned human tissue fragments, active pathogenic bacteria and viruses, cancerous cells, toxic and harmful chemicals, CO, acrylonitrile, formaldehyde, benzene series (especially toluene) In the surgical smoke of harmful substances such as phenols and phenols, long-term and repeated exposure will cause accumulation of toxic effects, so it should be taken seriously and protective measures should be taken

[0003] At present, chemical analysis methods for single toxic compounds in surgical smoke are not uncommon, and biological toxicity evaluation data are also reported.

However, there is a lack of invention and creation of quantitative evaluation methods for the combined biological toxicity of surgical smoke with complex components and a wide variety of toxic substances. In the future, it is expected to become a technical tool for ecological risk assessment and ecological pollution control of surgical smoke