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Online monitoring equipment and method for acute toxicity of water quality

An acute toxicity and water quality technology, applied in biochemical equipment and methods, color/spectral characteristic measurement, water testing, etc., can solve problems such as inability to guarantee long-term stable luminescence, short generations of luminescent bacteria, and non-recyclability, etc., to achieve structural Simple, convenient operation, and the effect of reducing losses

Inactive Publication Date: 2010-10-27
NANJING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several problems in its application: the generation of luminescent bacteria is very short, and its long-term stable luminescence cannot be guaranteed; the optical fiber probe inserted into the gel has poor sensitivity to detection; it cannot be reused, etc.

Method used

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  • Online monitoring equipment and method for acute toxicity of water quality
  • Online monitoring equipment and method for acute toxicity of water quality
  • Online monitoring equipment and method for acute toxicity of water quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of Luminescent Bacteria Biosensing Elements.

[0035] Inoculate the oblique luminescent bacteria strains into ASW liquid medium and cultivate them for about 16 hours at a temperature of 20°C and a rotation speed of 200r / min; take the above-mentioned bacterial suspension and dilute it 50 times. The optical density is about 0.1, and the diluted Add the solution into the sterilized cooled 40g / L sodium alginate solution, the volume ratio of the diluent and the sodium alginate solution is 1:1, and mix thoroughly at 20°C; use a syringe to drop the above mixed solution into 20g / L calcium chloride solution for 4 hours, the volume ratio of the mixed solution to the calcium chloride solution is 2%, wash with distilled water and soak in a solution containing 30g / L NaCl and 0.5g / L CaCl 2 in aqueous solution and stored at 4°C for later use.

[0036] Put the preserved luminescent bacterial sensitive element in an environment of 20°C for 10 minutes to recover ...

Embodiment 2

[0037] Example 2: Water quality acute toxicity online monitoring equipment.

[0038] Such as figure 1As shown, the online monitoring equipment for acute toxicity of water quality includes a continuous culture system for luminescent bacteria, a sampling system, a drainage system, a program control system, a photoelectric detection system, a data processing and transmission system, and a data display system. The luminescent bacteria continuous culture system is composed of nutrient solution storage bottle 1, liquid storage tank 2, pump 7, reaction test tube 8 and hose; 2 is connected with the reaction test tube 8 through a hose, and the pump 7 controls the acceleration of the nutrient solution flow from the liquid storage tank 2 to the reaction test tube 8, and the reaction test tube 8 is placed in a dark room that can be opened and closed. The sample adding system is composed of a sample tank 3, a pump 6 and a hose. The sample tank 3 is connected to the reaction test tube 8 in...

Embodiment 3

[0042] Example 3: Continuous operation of luminescent bacteria biological sensitive element on distilled water.

[0043] Adopt the water quality acute toxicity online monitoring equipment described in embodiment 2 to the continuous monitoring of distilled water, the effect of monitoring is as follows image 3 shown. It shows that as the test progresses, the growth status of the bacteria will change accordingly, resulting in a change in the light suppression rate, but except for a few points, it can remain relatively stable within a certain period of time, and can be calculated and calculated based on the measured historical data. Set the dynamic alarm line to eliminate this effect and make the calculation more accurate.

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Abstract

The invention discloses online monitoring equipment for acute toxicity of water quality. The equipment comprises a luminescent bacteria continuous culture system, a sample feeding system, a liquid draining system, a photoelectric detection system and a data display system, and can realize automatic operation. The invention also discloses a method for performing online monitoring on the acute toxicity of the water quality by utilizing the equipment. The online monitoring equipment for the acute toxicity of the water quality of the invention has the characteristics of simple structure, low cost and convenient operation, and can be operated and maintained by non-professionals only after a simple training. By using the method, continuous and automatic acute toxicity monitoring of the water quality can be realized completely; sensitivity is equivalent to that of an international luminescent bacteria method; and monitoring steps are simplified greatly. By using the equipment and the method, an early warning is provided for a sudden environment accident and corresponding measures are taken timely so as to reduce loss greatly.

Description

technical field [0001] The invention relates to a device and method for on-line monitoring of acute toxicity of water quality, in particular to a device and method for on-line monitoring of acute toxicity of water quality based on immobilized luminescent bacteria biological sensitive elements, belonging to the field of environmental monitoring. Background technique [0002] With the development of industry and agriculture and the intensification of human activities, sudden environmental pollution accidents have occurred frequently in recent years. Due to the lack of online early warning measures, not only caused great economic losses to the local area, but also caused casualties. In order to strengthen the protection of river water and drinking water sources, and the early warning and management of industrial wastewater discharge, relevant regulations have been issued internationally, requiring continuous toxicity control of water quality. However, traditional toxicity tests...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N21/27C12M1/36C12M1/34C12Q1/02
Inventor 肖琳孙旭杨柳燕赵背生曹妮妮
Owner NANJING UNIV
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