Method for detecting residual pesticidal toxicity in fruits and vegetables

A pesticide residue and detection method technology, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of limited luminescence time and the detection of the toxicity of pesticide residues in fruits and vegetables, so as to avoid missed detection, The effect of prolonging the stable light emission time

Active Publication Date: 2013-05-01
通标标准技术服务有限公司
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  • Abstract
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Problems solved by technology

However, the stable luminescence time of Vibrio Q67 strain in Qinghai is limited, which a...

Method used

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  • Method for detecting residual pesticidal toxicity in fruits and vegetables

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Experimental program
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Effect test

Embodiment 1

[0030] NaCl 3.67g, NaH 2 PO 4 0.18g, MgSO 4 ·7H 2 O 0.18g, MgCl 2 ·6H 2 O 0.39g, CaCl 2 0.07g, NaHCO 3 3.41g, MgCO 3 0.53g, MgBr 20.07g, KCl 0.03g, yeast extract 5g, tryptone 5g, agar 20g, glycerol 3g, dissolve in 1000mL distilled water, adjust the pH to 4.5, make the first-generation slant medium, sterilize at 121℃ for 20min, the second-generation In addition to the same components of the first-generation slant medium, the slant medium was added with Na 2 HPO 4 0.18g, adjust the pH to 8.5, and sterilize at 121°C for 20min; transplant the bacteria of Vibrio Qinghai Q67 strain to the above-mentioned first-generation slant medium, cultivate it at 20°C for 16h, and use 0.85% normal saline to cover the bacterial lawn Wash off from the slant medium, then transfer to the second-generation slant medium, and cultivate in the same way to obtain the bacterial liquid for testing. Mash 10g of common cucumber purchased from farmers’ market into juice, add 40mL of distilled ...

Embodiment 2

[0032] NaCl 3.15g, NaH 2 PO 4 0.24g, MgSO 4 ·7H 2 O 0.29g, MgCl 2 ·6H 2 O 0.31g, CaCl 2 0.14g, NaHCO 3 2.72g, MgCO 3 0.47g, MgBr 2 0.19g, KCl 0.02g, yeast extract 4g, tryptone 4g, agar 23g, glycerol 5g, dissolve in 1000mL distilled water, adjust the pH to 4, make the first generation slant medium, sterilize at 121℃ for 20min, the second generation In addition to the same components of the first-generation slant medium, the slant medium was added with Na 2 HPO 4 0.24g, adjust the pH to 8, and sterilize at 121°C for 20min; transplant the bacteria of Vibrio Qinghai Q67 strain to the above-mentioned first-generation slant medium, cultivate it at 23°C for 15h, and use 0.85% normal saline to cover the bacterial lawn Wash off from the slant medium, then transfer to the second-generation slant medium, and cultivate in the same way to obtain the bacterial liquid for testing. Mashed 10g of ordinary apples purchased from farmers’ markets into juice, added 40mL of distill...

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Abstract

The invention relates to the field of food safety, in particular to a method for detecting residual pesticidal toxicity in fruits and vegetables. The method for detecting the residual pesticidal toxicity in the fruits and the vegetables is characterized by comprising the following steps: preparing luminescent bacteria, preparing a detection sample, performing luminescent detection on the luminescent bacteria, mixing a fruit and vegetable sample to be detected with strain liquid of a vibrio qinghaiensis Q67 strain, and judging the residual pesticidal toxicity in the detected fruit and vegetable sample. By the method, the detection time of the residual pesticidal toxicity in the detected fruit and vegetable sample is shortened and the stable luminescent time of the vibrio qinghaiensis Q67 strain is prolonged.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a method for detecting the toxicity of pesticide residues in fruits and vegetables. Background technique [0002] At present, the detection of pesticide residues in vegetables and fruits generally uses gas chromatography, liquid chromatography and gas chromatography / mass spectrometry, etc., using laboratory chromatographic analyzers, and professional personnel are required to complete the analysis work. At present, spectrophotometry is generally used to detect the toxicity of pesticide residues. This method is relatively complicated to operate. It often takes several hours, or even a day or two, to obtain the analysis results from sampling and sample processing. Moreover, the spectrophotometer has a complicated structure and cannot be applied. Rapid qualitative detection on site. Luminescent bacteria are a type of bacteria that can emit visible fluorescence under normal physiological ...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 黄山梅
Owner 通标标准技术服务有限公司
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