Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting residual pesticidal toxicity in fruits and vegetables

A pesticide residue and detection method technology, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of limited luminescence time and the detection of the toxicity of pesticide residues in fruits and vegetables, so as to avoid missed detection, The effect of prolonging the stable light emission time

Active Publication Date: 2013-05-01
通标标准技术服务有限公司
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stable luminescence time of Vibrio Q67 strain in Qinghai is limited, which affects the detection of pesticide residue toxicity in fruits and vegetables to a certain extent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting residual pesticidal toxicity in fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] NaCl 3.67g, NaH 2 PO 4 0.18g, MgSO 4 ·7H 2 O 0.18g, MgCl 2 ·6H 2 O 0.39g, CaCl 2 0.07g, NaHCO 3 3.41g, MgCO 3 0.53g, MgBr 20.07g, KCl 0.03g, yeast extract 5g, tryptone 5g, agar 20g, glycerol 3g, dissolve in 1000mL distilled water, adjust the pH to 4.5, make the first-generation slant medium, sterilize at 121℃ for 20min, the second-generation In addition to the same components of the first-generation slant medium, the slant medium was added with Na 2 HPO 4 0.18g, adjust the pH to 8.5, and sterilize at 121°C for 20min; transplant the bacteria of Vibrio Qinghai Q67 strain to the above-mentioned first-generation slant medium, cultivate it at 20°C for 16h, and use 0.85% normal saline to cover the bacterial lawn Wash off from the slant medium, then transfer to the second-generation slant medium, and cultivate in the same way to obtain the bacterial liquid for testing. Mash 10g of common cucumber purchased from farmers’ market into juice, add 40mL of distilled ...

Embodiment 2

[0032] NaCl 3.15g, NaH 2 PO 4 0.24g, MgSO 4 ·7H 2 O 0.29g, MgCl 2 ·6H 2 O 0.31g, CaCl 2 0.14g, NaHCO 3 2.72g, MgCO 3 0.47g, MgBr 2 0.19g, KCl 0.02g, yeast extract 4g, tryptone 4g, agar 23g, glycerol 5g, dissolve in 1000mL distilled water, adjust the pH to 4, make the first generation slant medium, sterilize at 121℃ for 20min, the second generation In addition to the same components of the first-generation slant medium, the slant medium was added with Na 2 HPO 4 0.24g, adjust the pH to 8, and sterilize at 121°C for 20min; transplant the bacteria of Vibrio Qinghai Q67 strain to the above-mentioned first-generation slant medium, cultivate it at 23°C for 15h, and use 0.85% normal saline to cover the bacterial lawn Wash off from the slant medium, then transfer to the second-generation slant medium, and cultivate in the same way to obtain the bacterial liquid for testing. Mashed 10g of ordinary apples purchased from farmers’ markets into juice, added 40mL of distill...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of food safety, in particular to a method for detecting residual pesticidal toxicity in fruits and vegetables. The method for detecting the residual pesticidal toxicity in the fruits and the vegetables is characterized by comprising the following steps: preparing luminescent bacteria, preparing a detection sample, performing luminescent detection on the luminescent bacteria, mixing a fruit and vegetable sample to be detected with strain liquid of a vibrio qinghaiensis Q67 strain, and judging the residual pesticidal toxicity in the detected fruit and vegetable sample. By the method, the detection time of the residual pesticidal toxicity in the detected fruit and vegetable sample is shortened and the stable luminescent time of the vibrio qinghaiensis Q67 strain is prolonged.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a method for detecting the toxicity of pesticide residues in fruits and vegetables. Background technique [0002] At present, the detection of pesticide residues in vegetables and fruits generally uses gas chromatography, liquid chromatography and gas chromatography / mass spectrometry, etc., using laboratory chromatographic analyzers, and professional personnel are required to complete the analysis work. At present, spectrophotometry is generally used to detect the toxicity of pesticide residues. This method is relatively complicated to operate. It often takes several hours, or even a day or two, to obtain the analysis results from sampling and sample processing. Moreover, the spectrophotometer has a complicated structure and cannot be applied. Rapid qualitative detection on site. Luminescent bacteria are a type of bacteria that can emit visible fluorescence under normal physiological ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/76
Inventor 黄山梅
Owner 通标标准技术服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com