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Factory production method of dendrobium officinale seedling

A Dendrobium officinale and production method technology, applied in the field of bioengineering, can solve the problems of poor protocorm proliferation stability, etc., and achieve the effects of simple and practical medium components, strong growth potential, and reduced pollution

Inactive Publication Date: 2013-04-10
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defect that protocorm proliferation stability is not strong during the tissue culture of Dendrobium candidum in the prior art, and to provide a factory production method for Dendrobium candidum seedlings to solve the above problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Material: wild Yandang species of Dendrobium candidum.

[0037] 2. Explant selection, pretreatment and disinfection methods: From May to October, cut the annual stems from the vigorously growing and disease-free wild Yandang plants, remove the leaves, and cut them into sections with the stem nodes as the center. Leave 1 cm above and below; rinse with running water for 15 minutes, disinfect with 75% ethanol for 30 seconds on the ultra-clean workbench, and then use 0.1% HgCl 2(Add 2~3 drops of Tween-20) Soak and sterilize for 6 minutes, rinse with sterile water 3 times, and dry the water with sterile filter paper. Peel off the axillary buds with a scalpel, and then put 0.1% HgCl 2 (add 2~3 drops of Tween-20) for 6 minutes, rinse with sterile water for 3 times.

[0038] 3. Establishment of a sterile system: Cut off the above-mentioned stem segment by 0.5 cm at both ends, and inoculate it on MS solid medium for cultivation. The cultivation conditions are 25° C., 12 hou...

Embodiment 2

[0046] Take "Muge No. 1" (hybrid strain) optimal individual stem segments as explants. The basic operation method is consistent with embodiment 1. But its protocorm induction medium is: 1 / 2MS+6-BA2.0mg / L+sucrose 15g / L+coconut milk 10% (v / v)+agar 5g, pH5.6; protocorm subculture proliferation medium is: 1 / 2MS + sucrose 20g / L + coconut milk 10% (v / v) + agar 5g, pH5.6; Protocorm differentiation medium: 1 / 2MS + sucrose 20g / L + coconut milk 10% (v / v) + agar 5g , pH5.6; strong seedling rooting medium: 1 / 2MS + sucrose 20g / L + coconut milk 10% (v / v) + agar 5g + activated carbon 0.2~0.5g / L, pH5.6. After 40 days of strong seedling rooting and culture, the average number of roots is 5.1, the average plant height is 8.9 cm, and the average fresh weight is 3.2 grams. The survival rate of bottle seedling transplanting can reach more than 99.5%.

[0047] During the whole tissue culture process, the tissue culture room is designed according to the 100,000-class cleanliness, and adopts an ai...

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Abstract

The invention discloses a factory production method of a dendrobium officinale seedling, which comprises the steps of explant selection, pretreatment, sterilization, lateral bud germination, protocorm inducement, subculture, protocorm differentiation culture, strong seedling rooting culture, and bottle seedling transplant, and a specially-prepared culture medium is used in each step for culturing. The method has the benefits that an explant is easy to take; the sterilization efficiency is high; the growth coefficient is high and stable; components of the culture media are unique, concise and practical; the reproductive cycle is short; the production cost is low; and the produced seedling is stable in hereditary character, strong and tidy, and strong in growth vigor.

Description

technical field [0001] The invention relates to an industrial production method of dendrobium officinale seedlings, belonging to the technical field of bioengineering. Background technique [0002] Dendrobium officinale Kimura et Migo is a traditional Chinese herbal medicine in my country, which has the functions of nourishing yin and clearing heat, nourishing the stomach and promoting body fluid, moistening the lungs and improving eyesight, anti-cancer and anti-aging. Due to ecological damage and over-exploitation, wild Dendrobium candidum is on the verge of extinction, and its market price is getting more and more expensive. It is known as "plant panda" and "plant gold". In 1987, the State Council listed Dendrobium candidum as a key protection object. Since the beginning of the new century, with the breakthrough of Dendrobium candidum cultivation technology, the industry has developed rapidly. The current planting area of ​​Dendrobium candidum in China has exceeded 30,000 ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孔德栋
Owner ZHEJIANG UNIV
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