Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof

A technology for cryopreservation and antifreeze, which is applied in the field of duck egg yolk antifreeze and its preparation, and can solve the problems of long reproductive cycle of female animals, complicated and difficult production of frozen semen, and the like.

Active Publication Date: 2013-04-17
SHANDONG TIANLONG ANIMAL HUSBANDRY SCI & TECHNOLEGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention proposes a duck egg yolk antifreeze agent for cryopreservation of donkey semen, which solves the problems in the prior art that the bree...

Method used

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  • Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1) Each 100mL of antifreeze contains 3g of Tris, 1g of glucose, 1.5g of citric acid, 5mL of glycerin, 10mL of egg yolk, 450,000 IU of penicillin, 45,000 IU of streptomycin, and the balance is distilled water. Weigh each component, spare;

[0064] 2) Prepare the above-weighed Tris, glucose, and citric acid with distilled water to form a base solution;

[0065] 3) Add the penicillin sodium and streptomycin sulfate weighed in step 1) to the base liquid prepared above to prepare liquid I;

[0066] 4) Adjust the pH of the liquid I to 7.1, then filter and sterilize it, and put it in a refrigerator at 3°C ​​for later use;

[0067] 5) Take the liquid I before use, add the egg yolk weighed in step 1) to it, and prepare liquid II to obtain the duck egg yolk antifreeze agent for cryopreservation of donkey semen.

Embodiment 2

[0069] 1) Each 100mL of antifreeze contains 3.5g of Tris, 1.5g of glucose, 2.0g of citric acid, 7mL of glycerin, 20mL of egg yolk, 500,000 IU of penicillin, 50,000 IU of streptomycin, and distilled water as the balance. points, spare;

[0070] 2) Prepare the above-weighed Tris, glucose, and citric acid with distilled water to form a base solution;

[0071] 3) Add the penicillin sodium and streptomycin sulfate weighed in step 1) to the base liquid prepared above to prepare liquid I;

[0072] 4) Adjust the pH of the liquid I to 7.5, then filter and sterilize it, and put it in a refrigerator at 4°C for later use;

[0073] 5) Take the liquid I before use, add the egg yolk weighed in step 1) to it, and prepare liquid II to obtain the duck egg yolk antifreeze agent for cryopreservation of donkey semen.

Embodiment 3

[0075] 1) Each 100mL of antifreeze contains 4g of Tris, 2.5g of glucose, 3.0g of citric acid, 10mL of glycerin, 30mL of egg yolk, 550,000 IU of penicillin, 55,000 IU of streptomycin, and distilled water as the balance, and weigh each component ,spare;

[0076] 2) Prepare the above-weighed Tris, glucose, and citric acid with distilled water to form a base solution;

[0077] 3) Add the penicillin sodium and streptomycin sulfate weighed in step 1) to the base liquid prepared above to prepare liquid I;

[0078] 4) Adjust the pH of the liquid I to 7.8, then filter and sterilize it, and put it in a refrigerator at 5°C for later use;

[0079] 5) Take the liquid I before use, add the egg yolk weighed in step 1) to it, and prepare liquid II to obtain the duck egg yolk antifreeze agent for cryopreservation of donkey semen.

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Abstract

The invention provides a duck egg yolk anti-freezing agent for cryopreservation of donkey semen and a preparation method thereof. Each 100 milliliters of anti-freezing agent contains the following components: 3 to 4 grams of Tris, 1 to 2.5 grams of glucose, 1.5 to 3.0 grams of citric acid, 5 to 10 milliliters of glycerol, 10 to 30 milliliters of yolk, 450 to 550 thousand international units (IU) of penicillin, 45 to 55 thousand IU of streptomycin, and the balance of distilled water. The duck egg yolk anti-freezing agent solves the problems of long reproductive cycle of female animals, low artificial insemination rate, complex production operation of frozen donkey semen and high difficulty in the prior art.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a duck egg yolk antifreeze agent for donkey semen cryopreservation and a preparation method thereof. Background technique [0002] The combination of semen cryopreservation and artificial insemination technology plays a huge role in modern animal husbandry production. It can greatly reduce the number of male animals raised, reduce production costs, improve the utilization rate of excellent breeding male animals, and bring huge economic benefits to animal husbandry. benefit. At this stage, donkey breeding mainly adopts the method of self-breeding. Some skilled livestock improvers sometimes inseminate the collected semen (fresh semen) undiluted or at low times (1:2~4), and immediately inseminate the estrous female donkey. , it is suitable for areas where female donkeys have obvious seasonal estrus and a large number. Compared with this hand, the efficiency can be increased by 5-20 ti...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0221
Inventor 王长法张燕王玲玲朱涛涛张瑞涛鞠志花黄金明高运东仲跻峰侯明海王银朝闫金华
Owner SHANDONG TIANLONG ANIMAL HUSBANDRY SCI & TECHNOLEGY
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