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Tissue culture method of mosquito abjection vanilla

A mosquito repelling herb and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of susceptibility to virus, plant degeneration, limited speed, etc., and achieve the effect of short reproduction cycle, high survival rate and simple cultivation method

Inactive Publication Date: 2014-05-28
黎雁欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The value of mosquito-repelling herb has attracted more and more people's attention, but because mosquito-repelling herb is a fusion body, it cannot be sexually reproduced, and the cutting propagation uses a lot of materials and the speed is limited. Multiple cuttings will make the plant degenerate and susceptible to viruses. Propagation time and plant survival are limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The tissue culture method of mosquito-repellent herb comprises the steps:

[0019] 1) Explant treatment: select the young leaves of mosquito-repelling vanilla as explants, and leave the petiole length 1cm, then rinse with tap water for 25min, then treat with 75% alcohol for 2s under sterile conditions, and then use 0.1% mercury chloride Treat for 8 minutes, and finally rinse with sterile water 4 times;

[0020] 2) Inoculation and differentiation of explants: first use sterile filter paper to absorb the water from the explants treated in step 1), and then inoculate the petiole cuttings in MS+6-BA3.0mg / L+NAA0.3mg / L for differentiation On the culture medium, under the condition that the temperature is 25°C, the relative humidity is 70% and the light intensity is 1800lx, it is cultivated for 30 days to differentiate and sprout, and the differentiated buds are scored;

[0021] 3) Subculture: inoculate the differentiated buds up to 0.5 cm on MS+6-BA 0.4 mg / L proliferation me...

Embodiment 2

[0026] The tissue culture method of mosquito-repellent herb comprises the steps:

[0027] 1) Explant treatment: select the young leaves of mosquito-repelling vanilla as explants, and leave the petiole length 3cm, then rinse with tap water for 12min, then treat with 75% alcohol for 5s under sterile conditions, and then use 0.1% mercuric chloride Treat for 2 minutes, and finally rinse with sterile water 7 times;

[0028] 2) Inoculation and differentiation of explants: first use sterile filter paper to blot the explants treated in step 1) dry, and then inoculate the petiole cuttings in MS+6-BA3.0mg / L+NAA0.3mg / L for differentiation On the culture medium, under the condition that the temperature is 28°C, the relative humidity is 60% and the light intensity is 2500lx, it is cultivated for 45 days to differentiate and sprout, and the differentiated buds are scored;

[0029] 3) Subculture: inoculate the differentiated buds up to 1.5cm on MS+6-BA0.4mg / L proliferation medium, prolifera...

Embodiment 3

[0034] The tissue culture method of mosquito-repellent herb comprises the steps:

[0035] 1) Explant treatment: select the young leaves of mosquito-repelling vanilla as explants, and leave the petiole length 2cm, then rinse with tap water for 20min, then treat with 75% alcohol for 3s under sterile conditions, and then use 0.1% mercuric chloride Treat for 5 minutes, and finally rinse with sterile water for 6 times;

[0036] 2) Inoculation and differentiation of explants: first use sterile filter paper to blot the explants treated in step 1) dry, and then inoculate the petiole cuttings in MS+6-BA3.0mg / L+NAA0.3mg / L for differentiation On the culture medium, under the condition that the temperature is 27°C, the relative humidity is 65% and the light intensity is 2100lx, it is cultivated for 40 days until the buds are differentiated, and the buds are scored;

[0037] 3) Subculture: inoculate the differentiated buds up to 1.0 cm on MS+6-BA 0.4 mg / L proliferation medium, proliferate...

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Abstract

The invention discloses a tissue culture method of mosquito abjection vanilla. According to the method, tender leaves of the mosquito abjection vanilla are adopted as explants, and the method includes steps of treating the explants, inoculating and differentiating the explants, subculturing, performing rooting culture, and transplanting. The method has characteristics of simple culture method, short reproductive cycle, high survival rate and low cost, and is suitable for batch production.

Description

technical field [0001] The invention relates to a plant tissue culture method, and relates to a tissue culture method of mosquito repellent herb. Background technique [0002] Pelargonium citrosum Vanleenii, also known as net mosquito herb and mosquito repellent herb, belongs to the perennial transgenic plant of the genus Pelargonium in the genus Pelargonium citrosum. The citronellal emitted from its leaves has the effect of lemon incense and is often used to repel mosquitoes. Compared with traditional mosquito repellent and mosquito killing products, mosquito repellent vanilla is cheaper and more environmentally friendly. Mosquito repellent vanilla not only has a good mosquito repelling effect, but also has a strong air purification effect with its lemon fragrance, and has the effect of calming the nerves, refreshing the mind, and promoting sleep, and has no toxic side effects on the human body. Because it is evergreen all year round, it can be used as a bonsai for viewing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 黎雁欣
Owner 黎雁欣
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