Obligate halophile for processing high-salt oily waste liquor in petroleum industry

A technology of halophilic bacillus and waste liquid, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., and can solve the problems of reducing microbial activity and metabolism, difficulty in treating common microorganisms, and incomplete treatment, etc. , to achieve the effect of strong environmental adaptability, good industrial application prospects, and low processing cost

Active Publication Date: 2012-09-19
BC P INC CHINA NAT PETROLEUM CORP +2
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Problems solved by technology

[0004] The treatment of high-salt oil extraction waste liquid mainly includes physical, chemical and biological methods, but the physical and chemical methods have the disadvantages of incomplete treatment, high cost, easy to cause secondary pollution, etc., so it is difficult to promote in practice. , low investment, low operating costs, no pollution to the environment and other advantages, it has increasingly attracted the attention and attention of environmentalists from all over the world, and has become a hot spot and focus of research in the field of ecological environment at home and abroad.
In recent years, the use of microbial methods to treat petroleum pollutants in the environment has been carried out successively at home and abroad, mainly to screen highly efficient degrad

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  • Obligate halophile for processing high-salt oily waste liquor in petroleum industry

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Example Embodiment

[0020] Example 1. Screening and cultivation of Bacillus subtilis subsp.spizizenii Y-B CGMCC No. 4194 and the treatment effect on different oily waste liquids

[0021] Screening and cultivation of Bacillus subtilis subsp.spizizenii Y-B CGMCC No.4194:

[0022] Weigh 10g of oilfield drilling waste mud, put it into a triangular flask containing 90ml of sterile normal saline, and vibrate for 18-24 hours at a constant temperature at a temperature of 25-30°C. Use a sterile pipette tip to absorb 5ml of bacterial suspension and add it to the flask. In 45ml of halophilic culture medium, the mass percentages of the components of halophilic culture medium are: acid hydrolyzed casein 0.5%, yeast extract 1.0%, bacterial peptone 0.5%, trisodium citrate 0.3%, KCl 0.3%, MgSO 4 7H 2 O 1.8%, CaCl 2 0.02%, NaCl 5%, FeSO 4 7H 2 O solution is 0.0001%, and the rest is distilled water. Incubate at a temperature of 25-30°C for 2-3 days to obtain halophilic strains.

[0023] Take 5ml of the above bacterial l...

Example Embodiment

[0035] Example 2: Degradation ability of halophilic Bacillus strain Y-B CGMCC No. 4194 on waste drilling mud under optimized degradation conditions:

[0036] The pH of the drilling waste mud is around 9. According to the optimized degradation conditions of Bacillus subtilis subsp.spizizenii Y-B CGMCC No. 4194, the drilling waste mud with an oil concentration of about 600 mg / L is added with 8% halophilic by volume Bacillus subtilis subsp.spizizenii YB CGMCC No.4194, then add (NH 2 ) 2 CO nitrogen source and K 2 HPO 4 3H 2 O Phosphorus source, maintain N:P ratio of 1:1~3:1, and after shaking treatment at 25~30℃ for 7 days, test the change of oil content in the solution before and after the treatment. No other chemical treatment agent was added during the test. The test showed that after the optimization of the degradation conditions, the halophilic Bacillus subtilis subsp.spizizenii Y-B CGMCC No. 4194 had a 23% increase in the degradation ability of waste mud, from 51.21% to 70% to ...

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Abstract

The invention discloses a strain of obligate halophile Y-B, which is obtained from well drilling waste mud by enrichment, screening and culture, and aims at realizing high-efficient degradation of petroleum in high-salt oily waste liquor in the petroleum industry. The strain Y-B is milky white, is of a gram-positive bacterium, is shaped like a cell rod and can produce spores, the strain Y-B is identified as Bacillus subtilis subsp. spizizenii and has very high activity, a culture method is simple, and the strain Y-B has the advantages of easiness in obtainment, low cost and rapid growth, is suitable for weakly-alkaline and high-salt environments, has a better effect of removing the petroleum in the high-salt oily waste liquor and further has broad application prospects in the treatment ofthe high-salt oily waste liquor in the petroleum industry and oil overflow sudden accidents.

Description

technical field [0001] The invention relates to an original halophilic bacterial strain and its application, in particular to the application of the bacterial strain in the treatment of different types of high-salt oily waste liquids. Background technique [0002] At present, oil plays a pivotal role in the development of the national economy. With the rapid development of society and regional economy, the country's demand for oil energy continues to increase, making oil fields explored and developed on a large scale, and the pollution problems caused by oil development have become increasingly prominent and serious. [0003] In the process of petroleum exploration and development, different types of oily waste liquids are produced in drilling operations, downhole operations, oil production, and combined stations. Due to the different characteristics of crude oil and geological conditions, the properties of waste liquids are quite different, but they also have common charact...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125
Inventor 陈梅梅邓皓刘光全岳勇刘玉龙宋佳宇
Owner BC P INC CHINA NAT PETROLEUM CORP
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