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Cultivating method for improving multiplication coefficient of shoot

A technology of multiplication coefficient and cultivation method, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of high efficiency and short reproduction cycle

Inactive Publication Date: 2013-02-06
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the tissue culture of Acer sativa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0014] Specific embodiment one: the cultivation method that this embodiment improves the color wood maple stem bud proliferation coefficient realizes according to the following steps:

[0015] 1. Take the dormant branches of the adult maple tree and wash them with washing powder water, then rinse them with water, cut them into 2-3cm stem segments, and each segment has 1 axillary bud or 1 terminal bud, and then place the stem segments in the Soak in washing powder water for 10 minutes, stirring constantly, and then rinse under running water for 40 minutes;

[0016] 2. After the stem section is cleaned, soak it in an ethanol solution with a mass concentration of 75% for 30s on a super-clean workbench, and then sterilize it in 2% NaClO with 2 to 3 drops of Twen-20 for 20 minutes, and then use Rinse with sterile water 3 to 5 times;

[0017] 3. Use sterile filter paper to blot the water on the surface of the sterilized stem section in step 2, then use tweezers to peel off the bud ...

specific Embodiment approach 2

[0026] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the dormant branches on the adult mother tree of Acer cerevisiae in step one are scrubbed with washing powder water, then rinsed with water, and cut into 3cm stem sections. Other steps and parameters are the same as those in Embodiment 1.

Embodiment

[0028] The cultivation method for improving the stem bud proliferation coefficient of the color wood maple is realized according to the following steps:

[0029] 1. Take the dormant branches of the adult maple tree and wash them with washing powder water, then rinse them with water, cut them into 3cm stem segments, and each segment has 1 axillary bud or 1 terminal bud, and then put the stem segments in washing powder Soak in water for 10 minutes, stirring continuously, and then rinse under running water for 40 minutes;

[0030] 2. After the stem section is washed, soak it in an ethanol solution with a mass concentration of 75% for 30s on the ultra-clean workbench, then sterilize it in NaClO with a mass concentration of 2% added with 2 drops of Twen-20 for 20 minutes, and then use aseptic Wash with water 4 times;

[0031] 3. Use sterile filter paper to dry the water on the surface of the sterilized stem section in step 2, then use tweezers to peel off the bud scales until only...

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Abstract

The invention relates to a cultivating method for improving multiplication coefficient of a shoot, particularly to a cultivating method for improving multiplication coefficient of an acer mono shoot, which comprises the steps follows: 1), washing a resting shoot from an acer mono adult seed tree; 2), sterilizing a stem; 3), cultivating the sterilized stem to obtain the steam with a shoot; 4), cultivating the stem with the shoot to obtain the extended immature stem; and 5), cultivating the extended immature stem to obtain a plantlet. By adopting the resting sprouting shoot of the acer mono wild tree as the material, the research on the shoot multiplication and cultivation conditions are performed, and the cluster shoot induced cultivation system for directly multiplying the acer mono shoot is established, so as to lay a foundation of acer mono in vitro rapid propagation system. The reproductive cycle is short, the efficiency is high, the auxiliary shoot germination rate of the extended immature stem can reach 63.3%, the average height can reach 15.9 mm; and the shoot multiplication rate of the plantlet can reach 90%, the multiplication times can reach 3.19, and the shoot growth is normal.

Description

technical field [0001] The invention relates to a cultivation method for increasing the proliferation coefficient of stem buds. Background technique [0002] Acer mono Maxim, also known as Acer mono Maxim, is a deciduous tree belonging to the family Aceraceae. The tree has beautiful vigor, dense branches and leaves, beautiful leaf shape, red young leaves, and turns orange or red in autumn, which is very beautiful. In addition, the color maple has better shade tolerance, and can also grow well in the greening of factories and mines. It can be used as stump bonsai material. At present, the research on Acer syringus mainly focuses on sowing and raising seedlings, physiology of seed germination, cutting propagation, seedling cultivation, and flowering and fruiting. There are few reports on the tissue culture of Acer cerevisiae. In recent decades, tissue culture research on Acer species has made great progress. For example, foreign scholars have studied the somatic embryogen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王娜施志娟马欢官志玉杨玲沈海龙
Owner NORTHEAST FORESTRY UNIVERSITY
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