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Preparation method and use of CdS-Fe3O4-based electrochemiluminescence sensor

A cds-fe3o4 and ab-cds-fe3o4 technology, applied in the field of electrochemiluminescence detection, can solve the problems of complex operation steps of chromatographic analysis sample pretreatment instruments, and achieve the effects of wide signal response range, simple operation and high sensitivity

Active Publication Date: 2015-01-28
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity of chromatographic analysis is high, chromatographic analysis requires cumbersome sample pretreatment, pre-enrichment, and complicated instrument operation steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 A CdS-Fe based 3 o 4 Preparation method of electrochemiluminescence sensor

[0031] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, then ultrasonically clean it with ethanol, and then rinse it with ultrapure water;

[0032] (2) Dropping 4 μL, 0.5 mg mL -1 Ab-CdS-Fe 3 o 4 Antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0033] (3) Add 4 μL bovine serum albumin solution with a mass fraction of 1% dropwise to seal the non-specific active sites on the electrode surface, rinse the electrode surface with PBS buffer solution with pH 6.4, and dry at 4°C;

[0034] (4) Add 4 μL of the analyte solution dropwise, rinse the surface of the electrode with PBS buffer solution of pH 6.4, place it in a refrigerator at 4°C for 3 h to dry, and prepare a CdS-Fe-based 3 o 4 Preparation method of electrochemiluminescence sensor.

Embodiment 2

[0035] Example 2 A CdS-Fe based 3 o 4 Preparation method of electrochemiluminescence sensor

[0036](1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, then ultrasonically clean it with ethanol, and then rinse it with ultrapure water;

[0037] (2) Drop 4 μL, 1.0mg mL -1 Ab-CdS-Fe 3 o 4 Antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0038] (3) Add 4 μL of bovine serum albumin solution with a mass fraction of 2% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 PBS buffer solution, and dry at 4°C;

[0039] (4) Add 4 μL of the analyte solution dropwise, rinse the surface of the electrode with PBS buffer solution of pH 7.4, place it in a refrigerator at 4°C for 8 h to dry, and prepare a CdS-Fe-based 3 o 4 Preparation method of electrochemiluminescence sensor.

Embodiment 3

[0040] Example 3 A CdS-Fe based 3 o 4 Preparation method of electrochemiluminescence sensor

[0041] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, then ultrasonically clean it with ethanol, and then rinse it with ultrapure water;

[0042] (2) Dropping 4 μL, 2 mg mL -1 Ab-CdS-Fe 3 o 4 Antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0043] (3) Add 4 μL of bovine serum albumin solution with a mass fraction of 3% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 8.4 PBS buffer solution, and dry at 4°C;

[0044] (4) Add 4 μL of the analyte solution dropwise, rinse the surface of the electrode with PBS buffer solution of pH 8.4, place it in a refrigerator at 4°C for 12 h to dry, and prepare a CdS-Fe-based 3 o 4 Preparation method of electrochemiluminescence sensor.

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PUM

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Abstract

The invention relates to a preparation method and use of a CdS-Fe3O4-based electrochemiluminescence sensor and belongs to the field of electrochemiluminescence sensors. A CdS quantum dot is used as an electrochemiluminescence signal source, an antibody is effectively immobilized on the surface of a CdS-Fe3O4 nanometer compound by excellent biocompatibility and a large specific surface area of mesoporous ferroferric oxide, and the CdS quantum dot is immobilized on the surface of the mesoporous ferroferric oxide by a covalent bond so that an antibody capture substrate is obtained. The CdS-Fe3O4-based electrochemiluminescence sensor can realize fungaltoxin and hormone detection according to different electrochemiluminescence signal intensity values of different concentrations of objects to be detected.

Description

technical field [0001] The invention relates to a CdS-Fe based 3 o 4 Preparation method and application of electrochemiluminescence sensor. It specifically relates to a kind of CdS as a luminescent material, CdS-Fe 3 o 4 The preparation and application of an electrochemiluminescence sensor as a capture antibody substrate belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Mycotoxins are pollutants of agricultural products that widely exist in nature. Mycotoxins have strong nephrotoxicity, liver toxicity and neurotoxicity, and have teratogenic, carcinogenic and mutagenic effects. The presence of mycotoxins poses a huge threat to human and animal health as well as the development of animal husbandry. Hormones are special substances that are synthesized and secreted by certain cells of humans and animals and can regulate the physiological activities of the body, and together with the nervous system, regulate the metabolism an...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N21/76G01N33/588G01N33/74
Inventor 魏琴吕晓辉闫涛朱宝存杜斌马洪敏张勇吴丹
Owner UNIV OF JINAN
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