Detection method for 16 kinds of fungaltoxin

Abstract

The invention discloses a detection method for 16 kinds of fungaltoxin. The detection method comprises the following steps of extracting: weighing an accurate sample, putting into a centrifugal pipe,adding an extracting solution of an acetonitrile-water-acetic acid mixed solution, extracting in a vortex or oscillating mode, then centrifuging, sucking liquid supernatant into the centrifugal pipe,adding water into the centrifugal pipe, evenly mixing in a vortex mode, centrifuging, making the liquid supernatant pass through a polytetrafluoroethylene filter membrane and collecting to obtain filtrate A; adding stable isotopes: respectively and accurately sucking the filtrate A and a mixed standard solution of the 16 kinds of fungaltoxin into different internal insertion pipes, adding a mixedwork solution of 14 kinds of fungaltoxin stable isotopes and evenly mixing in a vortex mode. According to the detection method disclosed by the invention, a liquid chromatogram-tandem mass spectrometer is utilized for detection, instrument flexibility is remarkably improved, and the detection can be performed through a direct sample diluting and feeding mode; thus, target loss in a purification process is effectively avoided, dependence on a purifying material is reduced, detection cost is reduced, the sample pretreating efficiency is improved, and quickly detecting multiple components at thesame time is achieved.

Description

technical field [0001] The invention relates to a detection method, in particular to a liquid chromatography-tandem mass spectrometry method for the determination of 16 kinds of mycotoxins such as aflatoxin in feed, belonging to the detection field of feed and feed raw materials. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by fungi under suitable conditions, which can cause various acute and chronic diseases in humans and animals. The types of mycotoxins that mainly threaten human health include trichothecenes, fumonisins, ochratoxins, and aflatoxins. At present, most countries and regions have established strict limits for mycotoxins. my country also attaches great importance to the problem of mycotoxin pollution. In the newly revised "Food Safety Law" in 2015, biological toxins were clearly listed as key concerns for the first time. in pollutants. my country has also formulated four mycotoxin limit standards, namely deoxynivalenol (DO...

AI Technical Summary

Problems solved by technology

However, the ionization process of liquid chromatography-mass spectrometry is susceptible to matrix interference, which will cause matrix effects and affect the accurate quantification of toxins.

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