Method for simultaneously detecting various fungal toxins

Abstract

The invention relates to the technical field of detection of fungal toxins and particularly relates to a method for simultaneously detecting various fungal toxins. The method comprises the following steps: adding samples into an extracting agent for oscillating centrifugation, so as to obtain residues and supernate of the samples; adding methanol and pure water into the residues and taking supernate of the residues; mixing the supernate of the samples and the supernate of the residues to obtain supernate; mixing the supernate and diluent and filtering, so as to obtain extracting solution; enabling the extracting solution to slowly go through a complex immunoaffinity column, draining the extracting solution, performing gradient elution at least twice and collecting eluent; drying the eluent with by blowing nitrogen, so as to obtain to-be-detected solution after constant volume; simultaneously detecting the content of fungal toxins in the to-be-detected solution with LC-MS (liquid chromatography-mass spectrometry), respectively drawing a standard curve of each fungal toxin and finally calculating the content of the fungal toxins in the samples. According to the method, the defect that only one single fungal toxin can be detected with the conventional fungal toxin detection method is overcome, the detection cost is low, the detection efficiency is improved, and the loss and the pollution of a reagent are reduced.

Description

technical field [0001] The invention relates to the technical field of mycotoxin detection, in particular to a method for simultaneously detecting multiple mycotoxins. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi, and more than 400 types of mycotoxins have been discovered so far. The highly toxic mycotoxins mainly include aflatoxin B1 (AflatoxinB1, AFB1), ochratoxin A (OchratoxinA, OTA), zearalenone (Zearalenone, ZEA), deoxynivalenol (DON), fumonisins ( fomoni-sin, FB), T-2 toxin (T-2toxin, T-2) and so on. Over the years, some grains have been contaminated with many different mycotoxins during growing and storage. Even if some mycotoxins do not contaminate the same food crop at the same time, food processing often processes multiple raw materials into one food, resulting in the accumulation of mycotoxins. When people consume these foods, they may ingest multiple mycotoxins at the same time, and these toxins may There is an additive ...

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for simultaneously detecting multiple mycotoxins, which solves the defect that the existing mycotoxin detection method can only detect a single toxin, has low detection cost, improves detection efficiency, reduces reagent loss and pollution, and Full carbon-labeled stable isotopes are used as internal standards, and the detection cost of multiple toxins is too high for simultaneous determination of multiple toxins by liquid chromatography-mass spectrometry

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