Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles

Abstract

The invention provides a fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles, and belongs to the field of material chemical application. The invention has the main content of providing a simple and controllable preparation method of the Raman beacon molecular coding silver @ gold core-shell nanometer particles. A novel multiplex Raman sensing detector is built; the simultaneous fast specificity detection of two kinds or even various kinds of fungaltoxin is realized. The Raman beacon molecules are modified on the surfaces of silver nanometer particles; then, the growth of a layer of gold shell is performed; different Raman beacon molecular coding silver @ gold core-shell nanometer particles are prepared through regulating and controlling the pH of a buffer system and the types of the Raman beacon molecules; the Raman beacon molecule Raman signal intensity change and the generating mechanism under the electromagnetic field coupling effect between silver cores and gold shells are studied; the fungaltoxin aptamer specificity recognition principle and the good magnetic response of magnetic nanometer particles are combined; the multiplex Raman sensing detector is built; the simultaneous fast and specific detection of the aflatoxin and the ochratoxin on the basis of the Raman signals is realized for the first time.

Description

technical field [0001] The invention establishes a method for dual detection of mycotoxins based on Raman beacon molecule-encoded silver-gold core-shell nanoparticles, which belongs to the field of material chemistry applications. Background technique [0002] Mycotoxins are toxic secondary metabolites released by harmful microorganisms, which are carcinogenic, mutagenic and hepatotoxic, among which ochratoxin and aflatoxin are the most common mycotoxins in grain samples. At present, the commonly used detection techniques mainly include chromatography, fluorescence analysis and immunoassay, etc. However, the chromatography equipment is expensive, requires professional operators, and the method is time-consuming, which limits the application of chromatography; the fluorescence signal is easily affected by oxygen, humidity and external The interference of items affects the accuracy of detection; immunoassay relies on the specific affinity of antigen and antibody, the antibody ...

AI Technical Summary

Problems solved by technology

However, the length of the aptamers of ochratoxin and aflatoxin is longer, which will create a large gap between the nanoparticles, resulting in a weak Raman signal, which is not conducive to improving the detection sensitivity.

However, embedding Raman beacon molecules at the junction of the core and shell can significantly amplify the Raman signal of the beacon molecules, eliminating the need to precisely control the size of the gap between adjacent nanoparticles.

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