Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles

A silver nanoparticle and nanoparticle technology, applied in the field of dual detection of mycotoxins, can solve the problems of long length, unfavorable sensitivity, amplifying the Raman signal of beacon molecules, etc., and achieve the effect of specific detection

Inactive Publication Date: 2016-01-27
JIANGNAN UNIV
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Problems solved by technology

However, the length of the aptamers of ochratoxin and aflatoxin is longer, which will create a large gap between the nanoparticles, resulting in a weak Raman signal, which is not conducive to improving the detection sensitivity.
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Method used

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  • Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles
  • Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles
  • Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles

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Embodiment 1

[0020] (1) Preparation of p-aminothiophenol-encoded silver-gold core-shell nanoparticles

[0021] Silver nanoparticles with a particle size of 10 nm were prepared by sodium borohydride reduction method. 2 mL of silver nanoparticles were centrifuged at 8000 rpm for 10 min, the supernatant was removed, and the precipitate was resuspended in 200 μL of ultrapure water. Take 100 μL of silver nanoparticles and add 2 μL of 100 μM p-aminothiophenol to make a final concentration of 2 μM. React at room temperature for 12 h, centrifuge at 8000 rpm for 10 min to remove unmodified p-aminothiophenol, and resuspend the precipitate in 100 μL ultrapure water.

[0022] Take freshly prepared 100 μL of 100 mM citric acid solution and add it to 1 mL of 1% polyvinylpyrrolidone solution, and mix well. Use 200mM sodium hydroxide solution to adjust the pH of the solution to 9.5, then add 50 μL p-aminothiophenol-modified silver nanoparticles and 100 μL chloroauric acid solution with a concentration of...

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Abstract

The invention provides a fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles, and belongs to the field of material chemical application. The invention has the main content of providing a simple and controllable preparation method of the Raman beacon molecular coding silver @ gold core-shell nanometer particles. A novel multiplex Raman sensing detector is built; the simultaneous fast specificity detection of two kinds or even various kinds of fungaltoxin is realized. The Raman beacon molecules are modified on the surfaces of silver nanometer particles; then, the growth of a layer of gold shell is performed; different Raman beacon molecular coding silver @ gold core-shell nanometer particles are prepared through regulating and controlling the pH of a buffer system and the types of the Raman beacon molecules; the Raman beacon molecule Raman signal intensity change and the generating mechanism under the electromagnetic field coupling effect between silver cores and gold shells are studied; the fungaltoxin aptamer specificity recognition principle and the good magnetic response of magnetic nanometer particles are combined; the multiplex Raman sensing detector is built; the simultaneous fast and specific detection of the aflatoxin and the ochratoxin on the basis of the Raman signals is realized for the first time.

Description

technical field [0001] The invention establishes a method for dual detection of mycotoxins based on Raman beacon molecule-encoded silver-gold core-shell nanoparticles, which belongs to the field of material chemistry applications. Background technique [0002] Mycotoxins are toxic secondary metabolites released by harmful microorganisms, which are carcinogenic, mutagenic and hepatotoxic, among which ochratoxin and aflatoxin are the most common mycotoxins in grain samples. At present, the commonly used detection techniques mainly include chromatography, fluorescence analysis and immunoassay, etc. However, the chromatography equipment is expensive, requires professional operators, and the method is time-consuming, which limits the application of chromatography; the fluorescence signal is easily affected by oxygen, humidity and external The interference of items affects the accuracy of detection; immunoassay relies on the specific affinity of antigen and antibody, the antibody ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 赵媛罗耀东宋启军许春华
Owner JIANGNAN UNIV
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