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Surface-enhanced Raman detection method for mycotoxin based on silica-coated gold nanotriangle

A surface-enhanced Raman and mycotoxin technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of poor signal stability, single type of substrate material, and low detection sensitivity, so as to achieve stable signal and enhance Raman signal Stability, ease and effect

Inactive Publication Date: 2017-07-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Surface-enhanced Raman spectroscopy has been applied to the detection of trace substances such as pesticides, antibiotics, and bacteria, but there are relatively few studies on the detection of mycotoxins, and the type of substrate material is single, with low detection sensitivity, poor signal stability, and detection limits. need further improvement

Method used

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  • Surface-enhanced Raman detection method for mycotoxin based on silica-coated gold nanotriangle
  • Surface-enhanced Raman detection method for mycotoxin based on silica-coated gold nanotriangle
  • Surface-enhanced Raman detection method for mycotoxin based on silica-coated gold nanotriangle

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Embodiment 1

[0024] In order to further verify the sensitivity of the detection method prepared by the present invention to the detection of mycotoxins in food, examples of the present invention, taking aflatoxin B1 as an example, the specific operation steps are as follows:

[0025] Step 1) Preparation of gold nanotriangles: a three-step gold seed induction method is used to synthesize gold nanotriangles, specifically:

[0026] Gold Seed Solution 1:

[0027] 25 μL of 0.05M HAuCl 4 The solution was added to 4.7 mL, 0.1 M CTAC solution, and 300 μL of freshly prepared 0.01 M NaBH was added under vigorous stirring. 4 solution, put it at room temperature for 3h to remove excess NaBH 4 ;

[0028] Gold Seed Solution 2:

[0029] Dilute 1.6mL, 0.1M CTAC solution with 8mL ultrapure water, add 40μL, 0.05M HAuCl 4 Solution, quickly add 0.01M, 15μL sodium iodide solution;

[0030] Growth solution: 1 mL of 0.05M HAuCl 4 Add the solution to 80mL, 0.05M CTAC solution, and quickly add 0.01M, 600μL ...

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Abstract

The invention provides a surface-enhanced Raman detection method for mycotoxin based on a silica-coated gold nanotriangle. According to the method, a three-step seed induction method is employed for preparation of a gold nanotriangle, and the gold nanotriangle is labeled with a 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) signal molecule and coated with silica so as to prepare a gold@DTNB@silica surface Raman enhancing agent; a chitosan ferriferrous oxide (CS-Fe3O4) magnetic material is prepared at the same time; an enhanced substrate and the magnetic material are modified with mycotoxin aptamer so as to construct a mycotoxin detection system; when mycotoxin exists, gold@DTNB@silica and CS-Fe3O4 are bonded together via the effect of the specific recognition of the aptamer so as to form a CS-Fe3O4-aptamer-mycotoxin-gold@DTNB@silica detection system; with changes of the concentration of mycotoxin, the Raman signal of the system is not changed after magnetic separation; and thus, quantitative ultra-sensitive detection of mycotoxin in food is realized. The method is applicable to the technical fields of food safety, material chemistry and the like.

Description

technical field [0001] The invention relates to the technical fields of food safety, material chemistry and the like, in particular to a surface-enhanced Raman mycotoxin detection method based on silicon dioxide-coated gold nanotriangles. Background technique [0002] Mycotoxins (mycotoxins) are secondary metabolites produced by fungal growth. Cereals such as peanuts are particularly susceptible to contamination by aflatoxins due to improper storage and processing (including drying, processing, and storage processes), which have potential pathogenicity. Possibility of liver cancer, stomach cancer, kidney cancer. At present, the conventional methods for mycotoxin detection mainly contain biological identification method, but this method can only be used for qualitative detection, the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), its precision Poor, difficult to apply in practice; instrumental methods (gas chromat...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨明秀陈全胜李欢欢欧阳琴郭志明孙浩刘妍
Owner JIANGSU UNIV
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