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Preparation method and application of mycotoxin and hormone electrogenerated chemiluminescence sensor constructed based on NPCo/Co3O4-Au/RuSi@Ru(bpy)3<2+>

A co3o4-au, electrochemical technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problem of complex operation steps of chromatographic sample pretreatment instruments, and achieve a wide range of signal response. , high sensitivity, simple operation effect

Active Publication Date: 2015-08-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity of chromatographic analysis is high, chromatographic analysis requires cumbersome sample pretreatment, pre-enrichment, and complicated instrument operation steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 A NPCo / Co based 3 o 4 -Au / RuSiRu(bpy) 3 2+ Preparation method of constructed mycotoxin and hormone electrochemiluminescence sensor

[0033] (1) A glassy carbon electrode with a diameter of 4 mm was polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, ultrasonically cleaned with ethanol, and then rinsed with ultrapure water;

[0034] (2) Drop 6 μL, 0.5 mg mL -1 Antibody Incubation of Analyte NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ / Ab solution on the surface of the electrode, stored at 4°C until dry;

[0035] (3) Add 4 μL bovine serum albumin solution with a mass fraction of 1% dropwise to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH 6.4 PBS solution, and dry it at 4°C to prepare a Based on NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ Electrochemiluminescence sensors for mycotoxins and hormones.

Embodiment 2

[0036] Example 2 A NPCo / Co based 3 o 4 -Au / RuSiRu(bpy) 3 2+ Preparation method of constructed mycotoxin and hormone electrochemiluminescence sensor

[0037] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, clean it ultrasonically with ethanol, and rinse it with ultrapure water;

[0038] (2) Drop 6 μL, 1 mg mL -1 Antibody Incubation of Analyte NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ / Ab solution on the surface of the electrode, stored at 4°C until dry;

[0039] (3) Add 4 μL bovine serum albumin solution with a mass fraction of 2% dropwise to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH 7.4 PBS solution, and dry it at 4°C to prepare a Based on NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ Electrochemiluminescence sensors for mycotoxins and hormones.

Embodiment 3

[0040] Example 3 A NPCo / Co based 3 o 4 -Au / RuSiRu(bpy) 3 2+ Preparation method of constructed mycotoxin and hormone electrochemiluminescence sensor

[0041] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, clean it ultrasonically with ethanol, and rinse it with ultrapure water;

[0042] (2) Drop coating 6 μL, 2 mg mL -1 Antibody Incubation of Analyte NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ / Ab solution on the surface of the electrode, stored at 4°C until dry;

[0043] (3) Add 4 μL bovine serum albumin solution with a mass fraction of 3% dropwise to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH 8.4 PBS solution, and dry it at 4°C to prepare a Based on NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ Electrochemiluminescence sensors for mycotoxins and hormones.

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Abstract

The invention relates to a preparation method and application of a mycotoxin and hormone electrogenerated chemiluminescence sensor constructed based on NPCo / Co3O4-Au / RuSi@Ru(bpy)3<2+>, and belongs to the field of electrogenerated chemiluminescence sensors. RuSi@Ru(bpy)3<2+> is taken as an electrogenerated chemiluminescence signal source, an antibody is effectively supported to the surface of a nanometer composite NPCo / Co3O4-Au / RuSi@Ru(bpy)3<2+> by virtue of high biocompatibility and large specific surface area of a nanometer porous material NPCo / Co3O4-Au, and RuSi@Ru(bpy)3<2+> is supported to the surface of NPCo / Co3O4-Au to prepare an antibody capturing substrate through a covalent bond. Mycotoxins are detected according to different intensities of electrogenerated chemiluminescence signals of objects to be tested at different concentrations.

Description

technical field [0001] The present invention relates to a kind of based on NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ The preparation method and application of the constructed mycotoxin and hormone electrochemiluminescence sensor. Concretely involving a RuSiRu(bpy) 3 2+ As a luminescent material, the nanocomposite NPCo / Co 3 o 4 -Au / RuSiRu(bpy) 3 2+ The preparation and application of an electrochemiluminescence sensor as a capture antibody substrate belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Mycotoxins are pollutants of agricultural products that widely exist in nature. Mycotoxins have strong nephrotoxicity, liver toxicity and neurotoxicity, and have teratogenic, carcinogenic and mutagenic effects. The presence of mycotoxins poses a huge threat to human and animal health as well as the development of animal husbandry. Hormones are special substances that are synthesized and secreted by certain cells of humans and ani...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 魏琴吕晓辉王晓东马洪敏曹伟杜斌吴丹胡丽华李贺罗川南庞雪辉张勇
Owner UNIV OF JINAN
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