38 results about "Luminol chemiluminescence" patented technology

The invention relates to a gold nanoflower preparing method and the application of gold nanoflowers. The preparing method comprises the following steps that 1) under room temperature, polyphenol is added to water solution with the pH value of 7-12 to obtain mixed solution; 2) chloroauric acid and hydrogen peroxide are then added in sequence, shaking up is carried out to obtain reaction solution; 3) the reaction solution is centrifuged, supernatant is abandoned, and the gold nanoflowers are obtained after washing. The particle diameters of the gold nanoflowers obtained under different pH values are 50-200nm, stability is good, the gold nanoflowers can be used in a biological body as a medicine carrier and have good biocompatibility, the gold nanoflowers can be used in chemiluminescence analysis, the signal intensity of a luminal chemiluminescence system can be obviously enhanced, the gold nanoflowers can be used in surface-enhanced Raman scattering (SERS) analysis, and the sensitivity is better than that of spherical gold nanoparticles. The gold nanoflower preparing method has the advantages that raw materials are easy to get, reaction conditions are mild, efficiency is high, speed is high, and batch preparing and producing are benefited.

The invention discloses a method for enhancing luminol chemoluminescence by using copper oxide nanoparticles and is characterized in that the method comprises the following steps of: firstly pumping a copper oxide nanoparticle colloid solution and a hydrogen peroxide solution into a mixer for mixing through peristaltic pumps respectively, then mixing with a luminol solution, loading the resulting mixture to a flow cell for reaction, and detecting chemoluminescence intensity by a photomultiplier tube. The copper oxide nanoparticles are added to the luminol-hydrogen peroxide system, so as to significantly enhance chemoluminescence signals. The luminol-hydrogen peroxide-copper oxide nanoparticle flow injection chemiluminescence detection can be applied to glucose content determination, with a linear range of 5 mumol/L to 60 mumol/L.

The invention relates to a chemical luminous sensor and a method for detecting glucose by employing the chemical luminous sensor and can be applicable to clinical glucose detection. According to the method, glucose oxidase, a chemical luminous agent (luminol) and a catalyst are simultaneously fixed, and the luminous reaction conditions of the luminol are widened, so that the traditional alkaline substrate is expanded to a weakly acidic substrate, and the sensitivity and service life of the chemical luminous sensor are guaranteed; and moreover, the chemical luminous sensor has the characteristics of easy operation, low cost and wide linear range.

The present invention relates to a dual functionalized nanometer material, a preparation method and uses thereof, particularly to a dual functionalized noble metal nanometer material, which comprises noble metal nanoparticles, a luminescence segment derived from a luminol chemiluminescence reagent represented by a general formula (I) and a luminescence enhanced segment derived from a coordination metal ion chelate containing mercapto or amino, the luminescence segment derived from the luminol chemiluminescence reagent represented by the general formula (I) and the luminescence enhanced segment derived from the coordination metal ion chelate containing mercapto or amino are linked on the surface of the noble metal nanoparticles, A represents C6-C14 aryl and R1 and R2 can independently represent hydrogen, and linear or branched (C1-C30) alkyl with the amino substituted terminal or the unsubstituted linear or branched (C1-C30) alkyl in the formula, and the premise condition is that NR1R2 has at least one NH2 terminal. The dual functionalized noble metal nanometer material has the excellent luminescence characteristic. Compared with the dual functionalized noble metal nanometer material in the prior art, the luminescence characteristic of the dual functionalized noble metal nanometer material is substantially improved, and the unexpected technical effect is achieved. The general formula (I) is defined in the instruction.

The invention provides a method for quantitating stevioside in potassium ferricyanide-luminol chemoluminescence system. The method comprises making Fe(CN)3-6 solution, Luminol solution and stevioside standard solution with different concentration; employing spectrophotometer to perform full wavelength scanning on the stevioside standard solution between 190-1100nm; in the active pump, three-way mixing the carrier Luminol and stevioside standard solution at 45r/min flow speed via corresponding pipes and inputting to an analysis system; the slave pump injecting the Fe(CN)3-6 solution at 35r/min flow speed into the carrier flow via sixteen-way injection valve to mix with the carrier flow; the K3Fe(CN)6-Luminol is a margin luminary system (l0), sample enhancing signal (IS), getting peak value ( deltaI=IS-I0) to quantitate and the luminous intensity is displayed with number.

The invention discloses an enhanced chemiluminescence reagent which is composed of a solution A and a solution B. The solution A comprises 8-15 mmol/L luminol or luminol derivatives, 1-5 mmol/L sodium hydroxide, 0.1-1.5 mmol/L 4-[1H-1,2,4-triazole-1-based methyl] aniline hydrochloride, 0.1-0.8 mmol/L 1-naphthyl boric acid and a 0.1 mol/L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The solution B comprises 5-10 mmol/L hydrogen peroxide, 0.01%-0.05% of glutamic acid by volume and a 0.1 mol/L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The enhanced chemiluminescence reagent can remarkably improve the luminous intensity of a luminol chemiluminescence system and prolong the duration of luminescence.

The invention discloses a chemical luminescence enhanced type method for detecting pesticide residues, comprising the following steps of: preparing zymoid active particles and establishing a luminol chemical luminescence system, wherein the chemical luminescence enhancement is realized by adding organic phosphorus pesticide sample containing at least one phosphorus-sulfur single bond and having a series of concentrations and a free-radical scavenger into a zymoid active particle solution; then, adding a chemical illuminant, namely a luminol solution, wherein the chemical luminescence intensity of the obtained mixture is obviously enhanced compared with that of a blank in which the equal amount of the free-radical scavenger is added only at the moment; and accordingly, determining a linear relation between a sample concentration and the chemical luminescence intensity. According to the method disclosed by the invention, a principle of inhibiting the removing of free radicals by surface coordination is firstly utilized to design a chemical luminescence enhanced type sensor, which can be used for sensitive detection of a special pesticide component. Taking organic phosphorus pesticide ethoprophos as an example, the linear range is 0.1-1000 nmol/L and 1-100 micromoles/liter and the detection limit is 0.1 nmol/L.

The invention relates to a method for qualitatively and quantitatively detecting vomitoxin DON in cereals by directly competing chemiluminescence immunity. The detection method comprises the steps ofpreparing enzyme-labeled vomitoxin DON antibody, directly competing chemiluminescence immunity detection, establishing of a working curve equation and quantitatively detecting vomitoxin DON of a grainsample to be detected. According to an improved sodium periodate method in the method, a saturated NaBH4 methanol solution is adopted to carry out reduction reaction, the defects that the NaBH4 aqueous solution is prone to oxidizing and cannot be stored for a long time are overcome, so that the limitation of the current preparation of the solution is avoided; a direct competitive chemiluminescence immunity detection technology improves the detection efficiency and sensitivity; a highly sensitive luminol chemiluminescence substrate is adopted to replace a traditional TMB substrate, and thus the reaction sensitivity is greatly improved; by establishing the working curve, the concentration of vomitoxin DON in the sample can be quickly and accurately obtained, the complexity of detection andcalculation is reduced, and the method is applicable to a wide range of grassroots detection work.

The invention discloses a luminol chemiluminescence system based on a paper matrix and a method for detecting a phenolic antioxidant. The chemiluminescence system is composed of a Co Fe PBA NAFSs aqueous solution, a luminol working solution and a paper matrix containing hydrophilic and hydrophobic regions, wherein the Co Fe PBA NAFSs are KCoFe self-assembled nano particles with an anisotropic cubic frame structure, and the structural formula of the Co Fe PBA NAFSs is shown in the specification; the concentration of the Co Fe PBA NAFSs aqueous solution is 0.5-3.5 mg/mL, and the luminol workingsolution is a 0.4-1.8 mmol/L luminol solution prepared from a sodium hydroxide aqueous solution of which the pH value is 9.5-12.2. The chemiluminiscence system can catalyze luminol to generate strongchemiluminiscence on a paper substrate without hydrogen peroxide, when the phenolic antioxidant exists in the system, the chemiluminiscence system has an inhibiting effect on chemiluminiscence intensity, and the chemiluminiscence system can be used for detecting the phenolic antioxidant. The chemiluminiscence system has the advantages of rapidness, simplicity, convenience, high sensitivity, smallreagent dosage, small pollution and the like when being used for detecting the phenolic antioxidant.

The invention discloses a multifunctional integral catalyst, a synthesis method and application and relates to the field of catalysts. The multifunctional integral catalyst has the chemical formula ofC36H44Co3N4O16; each asymmetric unit consists of one flexible CBBA ligand, two Co(II) atoms, one mu-O atom, two coordinated water molecules and an N,N-dimethyl formamide molecules; by adopting a modethat organic functional components are introduced, and through controllable self-assembling process, on one hand, a three-core Co3O active unit is established, and on the other hand, a one-dimensional nano pore structure is established; and in addition, through potential coordinate omission, a multifunctional integral catalyst material can be prepared. The multifunctional integral catalyst is simple in synthesis method, gentle in reaction condition and easy in large-scale production. The multifunctional integral catalyst is mainly applied to three fields of epoxy compound open loop reactions,thioether controllable oxidation and luminal chemical light emission.

The invention discloses application of ascorbic acid peroxidase 1 in catalysis of luminol chemiluminescence reaction, and belongs to the technical field of plant immunity. The invention discloses the activity of ascorbic acid peroxidase 1 for catalyzing oxidation of luminol for the first time, and the ascorbic acid peroxidase 1 can be developed into a luminol-H2O2 chemiluminescence reaction catalyst. Based on the characteristic of stable expression of ascorbic acid peroxidase 1 in plant cells, the dynamic state and content of intracellular active oxygen can be monitored in real time by using a luminol chemiluminescence method, and the model is used for screening induction factors capable of causing burst-out of the intracellular active oxygen.

The invention provides application of N-hydroxysuccinimide as a light emitting coreactant, and belongs to the technical field of chemiluminescence analysis. The N-hydroxysuccinimide can be used as the light emitting coreactant to detect Co<2+>, luminol and NHS. The detection method has the advantages of high sensitivity, simple operation and fast analysis. NHS of the invention as a novel and efficient luminol chemiluminescent coreactant is capable of producing a chemiluminescent signal about 22 times stronger than a luminol-H2O2 chemiluminescent system; and the luminol-NHS chemiluminescent system can selectively enhance light emission in the presence of Co<2+>, so that the coreactant can be applied to efficient and selective detection of Co<2+>, luminol and NHS.

The present invention relates to clinical blood detecting and analyzing technology, and is the cTnI Luminol chemiluminescence immunological analysis detecting method with the system including self made cardiac muscle calcium protein resisting antibody I(cTnI) to label horseradish peroxidase, cinnamic acid as luminescence intensifier, tetreaphenyl sodium borate as synergistic intensifier and horseradish peroxidase as catalyst for Luminol-H2O2 luminescence. Compared with available ELISA process, the said CLIA process of the present invention has no obvious difference, correlation coefficient of 0.9852 and diagnosis coincidence rate of 96.15 %.

The invention relates to a chemical luminescent reinforcing agent, which comprises hydroquinone, 3, 5-ditert-butyl salicylic acid and Tris-HCl buffer solution, wherein the dosage of the effective ingredient hydroquinone is between 1 and 100mMol/L, and the dosage of the 3, 5-ditert-butyl salicylic acid is between 0.1 and 50mMol/L, the ion concentration of the Tris-HCl buffer solution is between 0.1 and 5 Mol/L and pH value is between 7.5 and 10. The chemical luminescent reinforcing agent has the advantages that the chemical luminescent reinforcing agent has convenient use, low cost, remarkableimprovement on luminous intensity and luminous endurance time of a luminol chemical luminescent system, and can be widely applied to chemical luminescence detection for various biological macromolecules and biological micromolecules in clinical examination, biological research, agricultural and forest industries, animal husbandry and environmental science.