Enhanced chemiluminescence reagent

A chemiluminescence reagent, enhanced technology, applied in chemiluminescence/bioluminescence, analysis through chemical reaction of materials, etc., can solve the problems of sensitivity improvement, high cost of use, and insufficient stability of luminescent substrates, etc., to achieve Improve the sensitivity and stability, prolong the duration, and improve the effect of chemiluminescent intensity

Active Publication Date: 2014-01-08
SHENZHEN HONGMED INFAGEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The luminescent substrates currently on the market are not stable enough, the sensitivity needs to be improved, and / or the cost of use is high, which limits the application of chemiluminescent immunoassays

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of Enhanced Chemiluminescent Reagent

[0035] Solution A:

[0036] Luminol, 10 mmol / L

[0037] Sodium hydroxide, 3 mmol / L

[0038] 4-[1H-1,2,4-Triazol-1-ylmethyl]aniline hydrochloride, 1.0 mmol / L

[0039] 1-Naphthylboronic acid, 0.5mmol / L

[0040] 0.1mol / L Tris-HCl buffer, pH 8.5;

[0041] Solution B:

[0042] Hydrogen peroxide, 5 mmol / L

[0043] Glutamic acid, volume fraction 0.03%

[0044] 0.1mol / L Tris-HCl buffer, pH 8.5.

[0045] Mix the above solutions in equal volumes to obtain the enhanced chemiluminescence reagent of the present invention.

[0046] Test results: with the basic luminescent solution (A solution: luminol, 10 mmol / L, sodium hydroxide, 3 mmol / L, 0.1mol / L Tris-HCl buffer, pH 8.5; B solution: hydrogen peroxide, 5 mmol / L L, 0.1mol / L Tris-HCl buffer solution, pH 8.5, mixed in equal volumes) compared, after adding horseradish peroxidase 5pg respectively, the enhanced chemiluminescent reagent of the present invention effecti...

Embodiment 2

[0049] Embodiment 2: the preparation of enhanced chemiluminescence reagent

[0050] Solution A:

[0051] Luminol monosodium salt, 8mmol / L

[0052] Sodium hydroxide, 1mmol / L

[0053] 4-[1H-1,2,4-Triazol-1-ylmethyl]aniline hydrochloride, 0.2 mmol / L

[0054] 1-Naphthylboronic acid, 0.1mmol / L

[0055] 0.1mol / L Tris-HCl buffer, pH 7.5;

[0056] Solution B:

[0057] Hydrogen peroxide, 3mmol / L

[0058] Glutamic acid, volume fraction 0.01%

[0059] 0.1mol / L Tris-HCl buffer, pH 7.5.

[0060] Mix the above solutions in equal volumes to obtain the enhanced chemiluminescence reagent of the present invention.

[0061] Test results: with the basic luminescence solution (A solution: luminol monosodium salt, 8mmol / L, sodium hydroxide, 1 mmol / L, 0.1mol / L Tris-HCl buffer, pH 7.5; B solution: hydrogen peroxide, 3 mmol / L, 0.1mol / L Tris-HCl buffer solution, pH 7.5, mixed in equal volumes) compared to that after adding horseradish peroxidase 5pg respectively, the enhanced chemiluminescent ...

Embodiment 3

[0064] Embodiment 3: the preparation of enhanced chemiluminescence reagent

[0065] Solution A:

[0066]Luminol, 15 mmol / L

[0067] Sodium hydroxide, 5 mmol / L

[0068] 4-[1H-1,2,4-Triazol-1-ylmethyl]aniline hydrochloride, 1.5 mmol / L

[0069] 1-Naphthylboronic acid, 0.8mmol / L

[0070] 0.1mol / L Tris-HCl buffer, pH 8.0;

[0071] Solution B:

[0072] Hydrogen peroxide, 10mmol / L

[0073] Glutamic acid, volume fraction 0.05%

[0074] 0.1mol / L Tris-HCl buffer, pH 8.0.

[0075] Mix the above solutions in equal volumes to obtain the enhanced chemiluminescence reagent of the present invention.

[0076] Test results: with the basic luminescence solution (A solution: luminol, 15mmol / L, sodium hydroxide, 5 mmol / L, 0.1mol / L Tris-HCl buffer, pH 8.0; B solution: hydrogen peroxide, 10 mmol / L , 0.1mol / L Tris-HCl buffer solution, pH 8.0, mixed in equal volumes) compared to, after adding horseradish peroxidase 10pg respectively, the enhanced chemiluminescent reagent of the present invent...

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PUM

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Abstract

The invention discloses an enhanced chemiluminescence reagent which is composed of a solution A and a solution B. The solution A comprises 8-15 mmol/L luminol or luminol derivatives, 1-5 mmol/L sodium hydroxide, 0.1-1.5 mmol/L 4-[1H-1,2,4-triazole-1-based methyl] aniline hydrochloride, 0.1-0.8 mmol/L 1-naphthyl boric acid and a 0.1 mol/L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The solution B comprises 5-10 mmol/L hydrogen peroxide, 0.01%-0.05% of glutamic acid by volume and a 0.1 mol/L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The enhanced chemiluminescence reagent can remarkably improve the luminous intensity of a luminol chemiluminescence system and prolong the duration of luminescence.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to an enhanced chemiluminescence reagent for chemiluminescence immunoassay. Background technique [0002] Chemiluminescence immunoassay (CLIA) is a combination of chemiluminescence system and immune reaction, which is used for the detection and analysis of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. It is a newest immunoassay technique developed after radioimmunoassay, enzyme immunoassay, fluorescent immunoassay and time-resolved fluorescent immunoassay. Chemiluminescent immunoassay has the characteristics of high sensitivity, simple and fast, linear and stable, safe and harmless. [0003] Luminol, Isoluminol and their derivatives are the first chemiluminescent substances used in chemiluminescence. The chemical name of luminol is 3-aminophthaloylhydrazide, and its luminescent properties were first reported by Albrecht. Such subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 侯志波
Owner SHENZHEN HONGMED INFAGEN CO LTD
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