Method for enhancing luminol chemoluminescence using copper oxide nanoparticles

A technology of nano-copper oxide and luminol chemistry, which is applied in the field of analytical chemistry and nanometers, can solve the problems of slow reaction speed and low luminous intensity, and achieve the effects of less sample demand, high sensitivity and fast detection speed
CN102507543BInactive Publication Date: 2013-12-04FUJIAN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
FUJIAN MEDICAL UNIV
Publication Date
2013-12-04
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for enhancing luminol chemoluminescence by using copper oxide nanoparticles and is characterized in that the method comprises the following steps of: firstly pumping a copper oxide nanoparticle colloid solution and a hydrogen peroxide solution into a mixer for mixing through peristaltic pumps respectively, then mixing with a luminol solution, loading the resulting mixture to a flow cell for reaction, and detecting chemoluminescence intensity by a photomultiplier tube. The copper oxide nanoparticles are added to the luminol-hydrogen peroxide system, so as to significantly enhance chemoluminescence signals. The luminol-hydrogen peroxide-copper oxide nanoparticle flow injection chemiluminescence detection can be applied to glucose content determination, with a linear range of 5 mumol / L to 60 mumol / L.
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Description

technical field

[0001] The invention relates to a method for enhancing luminol chemiluminescence by using nano copper oxide as a catalyst, and belongs to the field of analytical chemistry and nanotechnology. Background technique

[0002] Chemiluminescence is based on the fact that the molecules of certain substances in the reaction system absorb the energy released by the reaction and transition from the ground state to the excited state, and then return to the ground state from the excited state, while releasing energy in the form of light radiation. a luminescent phenomenon. The analysis method established based on the relationship between the molecular luminescence intensity and the content of the measured substance is called chemiluminescence analysis. In recent years, due to the advantages of low detection limit, rapid determination, and wide linear range, chemiluminescence has aroused widespread interest and attention in different fields, such as analytical chemistry,...

Claims

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