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Preparation method and application of gynecological tumor marker immune sensor constructed with ordered mesoporous carbon

An immunosensor and gynecological tumor technology, which is applied in the field of gynecological tumor marker immunosensor preparation, can solve the problems of human harm, long detection time, and poor popularity, and achieve excellent electrical conductivity, fast detection speed, and simple methods.

Inactive Publication Date: 2013-01-02
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies generally require complex monitoring instruments, the detection time is long, the popularity is poor, and some reagents are radioactive, which is harmful to the human body

Method used

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  • Preparation method and application of gynecological tumor marker immune sensor constructed with ordered mesoporous carbon
  • Preparation method and application of gynecological tumor marker immune sensor constructed with ordered mesoporous carbon
  • Preparation method and application of gynecological tumor marker immune sensor constructed with ordered mesoporous carbon

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Embodiment 1

[0061] A preparation of a gynecological tumor marker immunosensor constructed of ordered mesoporous carbon, comprising the following steps:

[0062] (1) Preparation of ordered mesoporous carbon nanomaterials, the steps are as follows:

[0063] 1) Take 0.4 g phenol, 1.4 mL 37% formaldehyde solution, 10 mL 0.1 mol L -1 Add NaOH into the three-necked flask respectively, and stir the reaction solution at 70 °C for 0.5 h;

[0064] 2) Dissolve 0.64 g of polyoxypropylene-polyoxyethylene copolymer F127 in 44 mL of ultrapure water in advance, stir to dissolve, add to 1) the reaction solution, react for 16 hours, the reaction solution turns from pink to red, when the precipitate appears , stop the reaction, and the reaction solution is cooled to room temperature;

[0065] 3) Add 173 mL of ultrapure water to the reaction solution obtained in 2), mix well, transfer to a reaction kettle, react in a muffle furnace at 130 °C for 24 h, cool to room temperature, wash by centrifugation, and d...

Embodiment 2

[0077] A preparation of a gynecological tumor marker immunosensor constructed of ordered mesoporous carbon, comprising the following steps:

[0078] (1) Preparation of ordered mesoporous carbon nanomaterials, the steps are as follows:

[0079] 1) Take 0.45 g phenol, 1.6 mL 37% formaldehyde solution, 11 mL 0.1 mol L -1 Add NaOH into the three-necked flask respectively, and stir the reaction solution at 70 °C for 0.5 h;

[0080] 2) Dissolve 0.72 g polyoxypropylene polyoxyethylene copolymer F127 in 49 mL ultrapure water in advance, stir to dissolve, add to 1) the reaction solution, react for 17 hours, the reaction solution turns from pink to red, when the precipitate appears , stop the reaction, and the reaction solution is cooled to room temperature;

[0081] 3) Add 195 mL of ultrapure water to the reaction solution obtained in 2), mix well, transfer to a reaction kettle, react in a muffle furnace at 130 °C for 24 h, cool to room temperature, wash by centrifugation, and dry in...

Embodiment 3

[0093] The preparation of a gynecological tumor marker immunosensor constructed of ordered mesoporous carbon comprises the following steps.

[0094] (1) Preparation of ordered mesoporous carbon nanomaterials, the steps are as follows:

[0095] 1) Take 0.50 g phenol, 1.8 mL 37% formaldehyde solution, 13 mL 0.1 mol L -1 Add NaOH into the three-necked flask respectively, and stir the reaction solution at 70°C for 0.5 h;

[0096]2) Dissolve 0.80 g polyoxypropylene polyoxyethylene copolymer F127 in 54 mL ultrapure water in advance, stir to dissolve, add to 1) the reaction solution, react for 18 hours, the reaction solution turns from pink to red, when the precipitate appears , stop the reaction and cool to room temperature;

[0097] 3) Add 217 mL of ultrapure water to the reaction solution obtained in 2), mix well, transfer to a reaction kettle, react in a muffle furnace at 130 °C for 24 h, cool to room temperature, wash by centrifugation, and dry in vacuum at room temperature to...

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Abstract

The invention relates to a preparation method and an application of a gynecological tumor marker immune sensor constructed with ordered mesoporous carbon. The invention belongs to the technical field of novel nano-grade functional material and biosensor. The preparation method is characterized by: (1) the preparation of an ordered mesoporous carbon nano-grade material; (2) the preparation of an ordered mesoporous carbon-toluidin blue composite material; and (3) the preparation of the gynecological tumor marker immune sensor. The gynecological tumor marker immune sensor provided by the invention has the advantages of fast identification, high sensitivity, low detection limit, low cost, and convenient operation. With the gynecological tumor marker immune sensor, high-sensitivity, specific, rapid, and accurate detections of various gynecological tumor markers can be realized. Sample determination can be finished in 3min, such that batched sample determination can be realized within a short period of time.

Description

technical field [0001] The invention relates to the preparation and application of a gynecological tumor marker immunosensor constructed with ordered mesoporous carbon. Specifically, based on ordered mesoporous carbon nanomaterials and ionic liquids, a label-free electrochemical immunosensor for detecting a variety of gynecological tumor markers is constructed, which is used to detect gynecological tumor markers in serum. It belongs to the field of new nano functional material and biosensing technology. Background technique [0002] Tumor is a long-term effect of internal and external pathogenic factors on human tissue cells, which leads to mutations in the genetic material of the cells - deoxyribonucleic acid (DNA), which makes the growth and division of cells out of control, resulting in abnormal proliferation and function. out of tune. Tumor is a serious threat to people's health, and it is a major research topic that has received much attention in the medical field in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 魏琴范海霞李贺张勇吴丹杜斌于海琴马洪敏庞雪辉王志玲罗川南李慧芝范大伟曹伟朱沛华张永芳
Owner UNIV OF JINAN
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