Kit for rapid detection of organophosphorus pesticide residues and its application method

A technology of organophosphorus pesticides and kits, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., which can solve the problems of false negative results, complicated operation process, expensive instruments, etc., and increase the linear range of detection. Wide, high detection sensitivity, fast detection effect

Inactive Publication Date: 2016-09-07
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spectrometry and chromatography are national standard methods for confirmatory detection of organophosphorus pesticides in agricultural products. They have the characteristics of strong specificity and high sensitivity. Quite complicated and not suitable for on-site detection of organophosphorus pesticide residues in agricultural products
However, its disadvantage is that the reaction system is susceptible to interference from external phosphates, resulting in false negative results.
In addition, the defect of this detection method is that the repeatability and stability are still not ideal, and it cannot meet the needs of qualitative and quantitative detection.

Method used

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  • Kit for rapid detection of organophosphorus pesticide residues and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This embodiment relates to a method for rapidly detecting organophosphorus pesticide residues using a kit, comprising the following steps:

[0031] This example detects the residues of organophosphorus pesticides in rapeseed sold in the market.

[0032] 1. Sample pre-treatment, put 10 parts of rapeseed leaves on the market with a weight of less than 10 grams in a plastic or glass container with a screw cap, or use a commonly used can bottle. Add about 10 ml of pure water, shake for 3 minutes, and let it stand for 2 minutes, then take the supernatant and use it directly for testing;

[0033]2, the preparation of enzyme cryoprotectant: the preparation method of present embodiment is as follows: prepare 100 milliliters of 5mmol / L calcium chloride solutions earlier, then add 1 gram of sucrose, 1 gram of glycine, 5 milliliters of glycerol, bovine serum albumin 1 gram, Stir while adding the above ingredients until all the powdered reagents are completely dissolved, and then ...

Embodiment 2

[0039] This embodiment relates to a method for rapidly detecting organophosphorus pesticide residues using a kit, comprising the following steps:

[0040] This example detects the residues of organophosphorus pesticides in commercially available spinach.

[0041] 1, sample pretreatment, step is the same as step 1 of embodiment 1;

[0042] 2, the preparation of enzyme cryoprotectant: the preparation method of present embodiment is as follows: first prepare 100 milliliters of 10mmol / L calcium chloride solution, then add 2 grams of sucrose, 2 grams of glycine, 10 milliliters of glycerol, bovine serum albumin 2 grams. Stir while adding the above ingredients until all the powdered reagents are completely dissolved, and then store in a freezer at -25°C until use;

[0043] 3. Preparation of reaction enzyme liquid: Dilute the above three enzyme liquids with the above-mentioned enzyme cryoprotectant, that is, dilute the acetylcholinesterase containing 425 international enzyme activity...

Embodiment 3

[0048] This embodiment relates to a method for rapidly detecting organophosphorus pesticide residues using a kit, comprising the following steps:

[0049] This example detects the residues of organophosphorus pesticides in crystal pears sold in the market.

[0050] 1. Sample pretreatment: first cut the crystal pear into one-eighth size, and place it in a clean can. All the other steps are with the step 1 of embodiment 1;

[0051] 2, the preparation of enzyme cryoprotectant: the preparation method of present embodiment is as follows: first prepare 100 milliliters of 15mmol / L calcium chloride solution, then add 3 grams of sucrose, 3 grams of glycine, 15 milliliters of glycerol, bovine serum albumin 3 grams. Stir while adding the above ingredients until all the powdered reagents are completely dissolved, and then store in a freezer at -40°C for later use;

[0052] 3. Preparation of reaction enzyme liquid: Dilute the above three enzyme liquids with the above-mentioned enzyme cry...

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Abstract

The invention relates to a kit for rapidly detecting organophosphorus pesticide residues and a method for using the kit; the kit includes: C enzyme bottle, T enzyme bottle, C substrate reaction cup, T substrate reaction cup, the C enzyme bottle , T enzyme bottles are all sealed with enzyme bottle sealing caps, and are equipped with enzyme freeze-dried three-enzyme mixture; the C substrate reaction cup, T substrate reaction cup are equipped with acetylcholine, luminol and dimethyl Freeze-dried absorbent pads for benzidine. The present invention also relates to a method for rapidly detecting organophosphorus pesticide residues using the aforementioned kit. The invention uses acetylcholinesterase, choline oxidase and peroxidase as a catalytic system to hydrolyze and oxidize the acetylcholine chloride product with luminol to produce a chemiluminescent effect. Establish a method for sensitive testing of organophosphorus pesticides in agricultural products. The invention has the advantages of fast detection speed, simple operation process, good stability and repeatability, and does not require expensive instruments, and is very suitable for on-site monitoring of organophosphorus pesticides in farmers' markets.

Description

technical field [0001] The invention relates to a method for quickly detecting organophosphorus pesticide residues in food, in particular to a kit for rapidly detecting organophosphorus pesticide residues and a use method thereof. Background technique [0002] Pesticide residues in agricultural products, food and pharmaceuticals are still one of the important potential factors threatening human health. In China's agricultural production process, the annual use of pesticides still ranks first in the world, and the use of organophosphorus pesticides accounts for more than 70% of the total amount of pesticides used. According to reports in recent years, the unqualified rate of organophosphorus pesticides in vegetables and fruits sold in farmers’ markets in large provincial cities in China accounted for more than 14% in the first quarter, especially the pesticide residues in vegetables grown in greenhouses are higher . In the pesticide sampling monitoring, it was found that th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 刘国艳柴春彦王艺如管毓渝任慧鹏
Owner SHANGHAI JIAOTONG UNIV
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