Application of N-hydroxysuccinimide as light emitting coreactant

A technology of hydroxysuccinimide and co-reactant, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve problems such as poor stability and selectivity, and achieve fast analysis speed and sensitivity. High, easy-to-use effects

Inactive Publication Date: 2016-05-25
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, various metal ions and their complexes can interact with H 2 o 2 A reaction occur

Method used

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  • Application of N-hydroxysuccinimide as light emitting coreactant
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  • Application of N-hydroxysuccinimide as light emitting coreactant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The detection of embodiment 1NHS

[0030] 10μM luminol sodium bicarbonate-sodium carbonate buffer solution (concentration 0.10M, pH 11.5) was injected into the first sample flow line A and the second sample flow line B respectively at a flow rate of 1.25mL / min and the secondary water of the sample base, turn on the IFIS-C mode intelligent flow injection peristaltic pump C, and then add 10 μM NHS to the injection valve D, and the chemiluminescence detector E records the luminous intensity signal, and the luminous intensity has a certain linear relationship with the NHS concentration. Enable NHS testing.

Embodiment 2

[0031] The detection of embodiment 2NHS

[0032] 10μM luminol sodium bicarbonate-sodium carbonate buffer solution (concentration 0.10M, pH 11.5) was injected into the first sample flow line A and the second sample flow line B respectively at a flow rate of 1.25mL / min and the secondary water of the sample base, turn on the IFIS-C mode intelligent flow injection peristaltic pump C, and then add 2000 μM NHS to the injection valve D, and the chemiluminescence detector E records the luminous intensity signal, and the luminous intensity has a certain linear relationship with the NHS concentration. Enable NHS testing.

Embodiment 3

[0033] The detection of embodiment 3NHS

[0034] 10μM luminol sodium bicarbonate-sodium carbonate buffer solution (concentration 0.10M, pH 11.5) was injected into the first sample flow line A and the second sample flow line B respectively at a flow rate of 1.25mL / min and the secondary water of the sample base, turn on the IFIS-C mode intelligent flow injection peristaltic pump C, and then add 5000 μM NHS to the injection valve D, and the chemiluminescence detector E records the luminous intensity signal, and the luminous intensity has a certain linear relationship with the NHS concentration. Enable NHS testing.

[0035] figure 2 It is the linear relationship curve graph of luminous intensity and NHS concentration of embodiment 1-3 of the present invention, wherein experimental condition is c (luminol): 10 μ M; c (NHS): 10-5000 μ M; Photomultiplier tube voltage: 800V, this method is to NHS The limit of detection was 3 μM.

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Abstract

The invention provides application of N-hydroxysuccinimide as a light emitting coreactant, and belongs to the technical field of chemiluminescence analysis. The N-hydroxysuccinimide can be used as the light emitting coreactant to detect Co<2+>, luminol and NHS. The detection method has the advantages of high sensitivity, simple operation and fast analysis. NHS of the invention as a novel and efficient luminol chemiluminescent coreactant is capable of producing a chemiluminescent signal about 22 times stronger than a luminol-H2O2 chemiluminescent system; and the luminol-NHS chemiluminescent system can selectively enhance light emission in the presence of Co<2+>, so that the coreactant can be applied to efficient and selective detection of Co<2+>, luminol and NHS.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescent analysis methods, and in particular relates to the application of N-hydroxysuccinimide as a luminescent co-reactant. Background technique [0002] Chemiluminescence analysis is a commonly used analysis and detection method, which has the advantages of high sensitivity, wide linear range, simple equipment, convenient operation, fast analysis speed, low background noise, and low price. It has been widely used in many fields such as drug analysis, biological analysis and detection, environmental monitoring, food science and clinical medicine. [0003] Luminol-based chemiluminescence systems require oxidizing agents and activators for chemiluminescence. Some classic oxidants such as hydrogen peroxide, potassium permanganate, ferricyanide, periodate and oxygen, and classic activators include peroxidase, catalase, cytochrome c, hemoglobin, ozone molecules , transition metal ions and their com...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 徐国宝萨祺礡·穆罕默德李素萍
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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