Method for enhancing luminol chemoluminescence using copper oxide nanoparticles

A technology of nano-copper oxide and luminol chemistry, which is applied in the field of analytical chemistry and nanometers, can solve the problems of slow reaction speed and low luminous intensity, and achieve the effects of less sample demand, high sensitivity and fast detection speed

Inactive Publication Date: 2012-06-20
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems of slow reaction speed and low luminous intensity of traditional chemiluminescent substances, the present invention provides a new method for enhancing luminol chemiluminescence based on the catalytic activity of nano-copper oxide

Method used

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  • Method for enhancing luminol chemoluminescence using copper oxide nanoparticles
  • Method for enhancing luminol chemoluminescence using copper oxide nanoparticles
  • Method for enhancing luminol chemoluminescence using copper oxide nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Flow injection chemiluminescence analysis system such as figure 1 As shown, firstly, 2.0 mg / L nanometer copper oxide colloidal solution and 1.0 mmol / L hydrogen peroxide solution were transported into the mixer through a peristaltic pump for mixing, and then mixed with 0.5 mmol / L luminol (pH 11.5) solution, three These are mixed into the flow cell for reaction, and the intensity of the chemiluminescence produced is detected by a photomultiplier tube. In the blank control experiment, distilled water was used instead of the nano copper oxide colloidal solution. Such as figure 2 As shown, the nano-copper oxide can significantly enhance the luminol chemiluminescence signal, and the enhancement factor is about 400.

Embodiment 2

[0041] Flow injection chemiluminescence analysis system such as figure 1 As shown, firstly, 2.0 mg / L nano copper oxide colloidal solution and 1.0 mmol / L hydrogen peroxide solution were transported into the mixer through peristaltic pump and mixed, and then mixed with different pH values ​​(take the value between 10 and 13) 0.5 mmol / L The L luminol solution is mixed, and the three are mixed into the flow cell for reaction, and the intensity of the generated chemiluminescence is detected by a photomultiplier tube. Such as image 3 As shown, the chemiluminescence signal increases with the increase of pH and reaches a peak value at pH 11.5, and the luminescence signal decreases when the pH value continues to increase.

Embodiment 3

[0043] Flow injection chemiluminescence analysis system such as figure 1As shown, firstly, 2.0 mg / L nano copper oxide colloidal solution and 1.0 mmol / L hydrogen peroxide solution were transported into the mixer through peristaltic pump and mixed, and then mixed with different concentrations (take the value between 0.1 and 1.0 mmol / L) Lu Minol solution (pH 11.5) is mixed, and the three are mixed into the flow cell for reaction, and the intensity of chemiluminescence generated is detected by a photomultiplier tube. Such as Figure 4 As shown, the chemiluminescence signal increased with the increase of luminol concentration and reached the peak at the concentration of 0.5 mmol / L, and the luminescence signal decreased when the luminol concentration continued to increase.

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Abstract

The invention discloses a method for enhancing luminol chemoluminescence by using copper oxide nanoparticles and is characterized in that the method comprises the following steps of: firstly pumping a copper oxide nanoparticle colloid solution and a hydrogen peroxide solution into a mixer for mixing through peristaltic pumps respectively, then mixing with a luminol solution, loading the resulting mixture to a flow cell for reaction, and detecting chemoluminescence intensity by a photomultiplier tube. The copper oxide nanoparticles are added to the luminol-hydrogen peroxide system, so as to significantly enhance chemoluminescence signals. The luminol-hydrogen peroxide-copper oxide nanoparticle flow injection chemiluminescence detection can be applied to glucose content determination, with a linear range of 5 mumol/L to 60 mumol/L.

Description

technical field [0001] The invention relates to a method for enhancing luminol chemiluminescence by using nano copper oxide as a catalyst, and belongs to the field of analytical chemistry and nanotechnology. Background technique [0002] Chemiluminescence is based on the fact that the molecules of certain substances in the reaction system absorb the energy released by the reaction and transition from the ground state to the excited state, and then return to the ground state from the excited state, while releasing energy in the form of light radiation. a luminescent phenomenon. The analysis method established based on the relationship between the molecular luminescence intensity and the content of the measured substance is called chemiluminescence analysis. In recent years, due to the advantages of low detection limit, rapid determination, and wide linear range, chemiluminescence has aroused widespread interest and attention in different fields, such as analytical chemistry,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 陈伟洪磊刘爱林李光文刘建清林新华
Owner FUJIAN MEDICAL UNIV
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