A kind of analysis probe, its preparation method and application

A technology for analyzing probes and molecules, which is applied in the direction of analyzing materials, chemical instruments and methods, and analyzing materials by chemical reaction, which can solve the problem that the luminescence intensity cannot be improved to meet the requirements, and achieve good application prospects and reliability. Strong and reproducible effect

Active Publication Date: 2017-05-03
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it was found that adding this catalytic substance to the existing luminescent functionalized nanomaterials still failed to increase the luminous intensity to a level that meets the requirements.

Method used

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  • A kind of analysis probe, its preparation method and application
  • A kind of analysis probe, its preparation method and application
  • A kind of analysis probe, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0084] B. Preparation method of bifunctional noble metal nanomaterials

[0085] The present invention also provides a method for preparing the bifunctional noble metal nanomaterial of the present invention easily and rapidly, which comprises the following steps:

[0086] 1. Provide coordination metal ion chelates containing mercapto or amino groups

[0087] The mercapto or amino-containing coordination metal ion chelate is as defined above, which comprises a coordination metal ion and a chelating agent, wherein the chelating agent can be obtained by performing a chelating agent that does not contain a mercapto or amino group known in the art. Thilated or aminated in the system. The thiolation or amination of such chelating agents is known, see for example: Christophe Alric, Jacqueline Taleb, Géraldine Le Duc, Céline Mandon, Claire Billotey, Alice LeMeur-Herland, Thierry Brochard, Francis Vocanson, Marc Janier, Pascal Perriat, Stéphane Roux, and Olivier Tillement. J. Am. Chem...

Embodiment I

[0196] Embodiment 1: the preparation of bifunctional noble metal nanomaterial

[0197] Test Methods:

[0198] (1) transmission electron microscope;

[0199] Test method: transmission electron microscope (TEM, JEOL Ltd, JEOL-2010, Japan). Centrifuge the prepared sample at 12500*g for 15 minutes and remove the supernatant. After the obtained precipitate was dispersed in absolute ethanol solution, transmission electron microscope analysis was performed.

[0200] (2) thermogravimetric analysis and differential thermal analysis;

[0201] Test method: (TGA / DTG, Q5000 V3.15) Centrifuge the prepared sample, remove the supernatant after 12500*g for 15min. The obtained precipitate was dried under vacuum condition, and thermogravimetric analysis and differential thermal analysis were carried out.

[0202] (3) UV-Vis spectrum;

[0203] Test method: (Agilent 8453 ultraviolet-visible spectrometer) centrifuge the prepared sample, and remove the supernatant after 12500*g for 15 minute...

Synthetic example 1

[0208] Synthesis Example 1: Preparation of thiolated diethylenetriaminepentaacetic acid (chelating agent 1)

[0209] Dissolve 1g of diethylenetriaminepentaacetic dianhydride in 20mL of N,N-dimethylformamide, and heat to 70°C to obtain solution A. Dissolve 0.55g of 2-aminoethanethiol in 15mL of N,N-dimethylformamide, add 0.87mL of triethylamine, and heat to 70°C to obtain solution B. Add solution B to solution A, heat and stir at 70°C overnight. During the reaction process, the solution gradually changed from colorless to light yellow, indicating that diethylenetriaminepentaacetic dianhydride and 2-aminoethanethiol had undergone amidation reaction to generate a chelating agent with mercapto groups. After the solution was cooled to room temperature, it was placed in an ice bath, and the white precipitate was filtered off. The obtained filtrate was concentrated by rotary evaporation, and a white precipitate was obtained after adding chloroform. The resulting white precipitat...

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Abstract

The invention relates to an analysis probe, its preparation method and use. Specifically relates to an analytical probe, which comprises a bifunctional noble metal nanomaterial and a detection molecule, the bifunctional noble metal nanomaterial is directly connected to the detection molecule, or indirectly connected through a binding pair or a linking group, wherein the The bifunctional noble metal nanomaterial comprises noble metal nanoparticles; a luminescent segment derived from a luminol-based chemiluminescence reagent of general formula (I); and a luminescence-enhancing segment derived from a coordination metal ion chelate containing a sulfhydryl group or an amino group; The luminescent segment derived from the luminol-based chemiluminescence reagent of the general formula (I) and the luminescence-enhancing segment derived from the coordination metal ion chelate containing mercapto or amino groups are connected on the surface of the noble metal nanoparticles: formula Among them, A represents a C6-C14 aryl group; R1 and R2 can independently represent hydrogen, a linear or branched (C1-C30) alkyl group whose terminal group is substituted by an amino group or unsubstituted, provided that the NR1R2 has at least one NH2 terminal base. General formula (I).

Description

technical field [0001] The invention relates to a bifunctional noble metal nano material containing a chemiluminescence segment and a luminescence enhancement segment. The invention also relates to the preparation and application of the bifunctional noble metal nanometer material. Background technique [0002] Noble metal nanomaterials have unique optical properties, catalytic activity and biocompatibility, etc. These characteristics make noble metal nanomaterials widely used in the field of chemiluminescence analysis. The surface modification of the existing noble metal nanomaterials to have luminescent molecules on the surface, that is, the preparation of luminescent functionalized noble metal nanomaterials has attracted widespread attention. [0003] For example, it is known in the prior art to directly use luminol to reduce chloroauric acid, and obtain luminol-functionalized gold nanomaterials in one step (Cui, H.; Wang, W.; Duan, C.F.; Dong, Y.P.; Guo , J.Z.Chem.Eur.J...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/553C09K11/06
Inventor 崔华刘孟晓张宏丽舒江南
Owner UNIV OF SCI & TECH OF CHINA
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