Method for detecting Pb < 2 + > by Cu-MOF-luminol-H2O2 chemiluminescence system

A chemiluminescence, cu-mof-technology, applied in the field of chemical detection, can solve the problems of unsuitable trace or even lower concentration lead ion detection, low sensitivity, and high requirements for detection equipment, and achieves strong selectivity, high sensitivity, and high detection efficiency. low limit effect

Active Publication Date: 2020-07-31
中晋环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a Cu-MOF-luminol-H 2 o 2 Detection of Pb by Chemiluminescent System 2+ Methods

Method used

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  • Method for detecting Pb &lt; 2 + &gt; by Cu-MOF-luminol-H2O2 chemiluminescence system
  • Method for detecting Pb &lt; 2 + &gt; by Cu-MOF-luminol-H2O2 chemiluminescence system
  • Method for detecting Pb &lt; 2 + &gt; by Cu-MOF-luminol-H2O2 chemiluminescence system

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Experimental program
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Effect test

Embodiment 1

[0028] Dilute the preparation to give 1.0 x 10 -3 mol / L luminol reagent, and 30% H 2 o 2 Reagents were serially diluted to obtain 1.0×10 -3 mol / L H 2 o 2 Solution, ready to use when used. Dissolve 0.01 g Cu-DOBDC in 1 mL water to obtain 10 mg / mL Cu-DOBDC solution, take 0.5 mL Cu-DOBDC and 0.5 mL different concentrations of Pb to be tested 2+ The solution was mixed evenly and allowed to stand for 30 min. Measure 10 μL Cu-MOF / Pb 2+ Mix the solution to be tested in a small glass beaker, add 10 μL H 2 o 2 The reagent is placed in a chemiluminescence cassette, and 50 μL of luminol solution is added dropwise by flow injection method to detect chemiluminescence. The luminescence signal is detected by the photomultiplier tube, and the intensity of the light signal is output under the analysis software of the computer.

Embodiment 2

[0030] Dilute the preparation to give 1.0 x 10 -3 mol / L luminol reagent, and 30% H2 o 2 Reagents were serially diluted to obtain 1.0×10 -3 mol / L H 2 o 2 Solution, ready to use when used. Dissolve 0.01 g Cu-DOBDC in 1 mL water to obtain 10 mg / mL Cu-DOBDC solution, take 0.5 mL Cu-DOBDC and 0.5 mL different concentrations of Pb to be tested 2+ The solution was mixed evenly and allowed to stand for 30 min. Measure 5 μL Cu-MOF / Pb 2+ Mix the solution to be tested in a 96 microwell plate, add 5 μL H 2 o 2 The reagent is placed in a chemiluminescence cassette, and 25 μL of luminol solution is added dropwise by flow injection method to detect chemiluminescence. The luminescence signal is detected by the photomultiplier tube, and the intensity of the light signal is output under the analysis software of the computer.

Embodiment 3

[0032] Dilute the preparation to give 1.0 x 10 -3 mol / L luminol reagent, and 30% H 2 o 2 Reagents were serially diluted to obtain 1.0×10 -3 mol / L H 2 o 2 Solution, ready to use when used. Dissolve 0.01 g Cu-DOBDC in 1 mL water to obtain 10 mg / mL Cu-DOBDC solution, take 0.1 mL Cu-DOBDC and 0.1 mL different concentrations of Pb to be tested 2+ The solution was mixed evenly and allowed to stand for 30 min. Measure 2.5 μL Cu-MOF / Pb 2+ Mix the solution to be tested on Whatman No. 1 filter paper, add 2.5 μL H 2 o 2 The reagent was placed in a chemiluminescence cassette, and 20 μL of luminol solution was added dropwise by flow injection method to detect chemiluminescence. The luminescence signal is detected by the photomultiplier tube, and the intensity of the light signal is output under the computer analysis software. The results are as follows: figure 1 , image 3 shown.

[0033] Depend on figure 1 Luminol-H recorded by chemiluminescence 2 o 2 The chemiluminescence...

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Abstract

The invention discloses a method for detecting Pb < 2 + > by a Cu-MOF-luminol-H2O2 chemiluminescence system, belongs to the field of chemical detection, and can solve the problems that the existing detection method has low sensitivity and is not suitable for detection of trace or even lower-concentration lead ions and has high requirements on detection equipment in the prior art. Cu-DOBDC is obtained from a divalent metal ion Cu (II) and an organic ligand 2, 5-dihydroxy terephthalic acid (H2DOBDC) by using a hydrothermal method, and is introduced into a luminol-H2O2 chemiluminescence system toform the Cu-DOBDC-luminol-H2O2 chemiluminescence system. When Pb < 2 + > exists, Pb < 2 + > and Cu-DOBDC have stronger coupling capacity, Cu at an active site in Cu-DOBDC is released, chemiluminiscence of a luminol-H2O2 system is enhanced, and the signal enhancement degree is in direct proportion to the concentration of Pb < 2 + >. The method is easy to operate, high in sensitivity, low in detection limit, high in selectivity and suitable for on-site rapid detection.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, in particular to a Cu-MOF-luminol-H 2 o 2 Detection of Pb by Chemiluminescent System 2+ Methods. Background technique [0002] Lead is a heavy metal element that seriously endangers human health. It can affect various organs such as nerves, hematopoiesis, digestion, urinary, cardiovascular, reproduction, and bones. What is more serious is that it affects the growth and intellectual development of infants and young children. Brain functions such as cognitive function, neurobehavior, learning and memory, and severe cases cause dementia. Therefore, the research on the determination method of lead has important practical significance. [0003] The methods reported so far for the detection of lead ions include: ultraviolet-visible spectrophotometry, electrochemical analysis, atomic emission spectrometry and so on. However, the sensitivity of UV-visible spectrophotometry and electrochem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N1/38
CPCG01N21/76G01N1/38
Inventor 李泓锐侯越邓飞韩亮刘畅王越
Owner 中晋环境科技有限公司
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