Method for detecting chlorophenol pollutants by utilizing chemiluminescent method

A chemiluminescence method and chemiluminescence technology, which is applied in the field of analytical chemistry and optical DNA biosensing, can solve the problem of chemiluminescent signal reduction and achieve the effect of less sample consumption, low cost and high sensitivity

Inactive Publication Date: 2016-07-27
BEIJING RES CENT FOR AGRI STANDARDS & TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In our research, we found that chlorophenol pollutants can inhibit the luminescent system of G-quadruplex / Hemin peroxide mimic enzyme, hydrogen peroxide, and luminol, resulting in a decrease in chemiluminescent signal, and the chemiluminescent signal is consistent with There is a certain relationship between the concentration change
However, so far, there are no related research reports on the detection of chlorophenol pollutants based on G-quadruplex / Hemin peroxide mimetic enzyme.

Method used

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  • Method for detecting chlorophenol pollutants by utilizing chemiluminescent method
  • Method for detecting chlorophenol pollutants by utilizing chemiluminescent method
  • Method for detecting chlorophenol pollutants by utilizing chemiluminescent method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1G-quadruplex / Hemin peroxide mimetic enzyme

[0035] Dilute solid DNA powder (Tel22DNA) with 20mM Tris-HClO 4 Dissolve the buffer solution, mix it with a vortex oscillator, and place it in a water bath at 85°C for 5 minutes, take it out, cool it naturally, and let it stand at room temperature. 20mM Tris-KClO 4 The solution was diluted to 1 μmol, and after incubation for 2 hours, 1.2 μmol of hemin was added, mixed with a vortex shaker, and left for 2 hours to obtain G-quadruplex / Hemin peroxide mimetic enzyme with a concentration of 1 μmol.

Embodiment 2

[0037] 1. Mix the G-quadruplex / Hemin peroxide mimetic enzyme prepared in Example 1 with the luminol solution evenly using a vortex oscillator;

[0038] 2. Use Tris-KClO to pentachlorophenol 4 After diluting the buffer solution, with H 2 o 2 The solution is mixed evenly;

[0039] 3. After placing the mixed solution obtained in step 1 in the chemiluminescence detection cell of the weak chemiluminescence detector, quickly inject the mixed solution obtained in step 2 into the chemiluminescence detection cell to obtain a mixed luminescent system for chemiluminescence signal detection.

[0040] Among them, in order to verify the influence of different concentrations of pentachlorophenol on the luminescent signal, the concentrations of pentachlorophenol in the mixed luminescent system were respectively 0nmol / L, 10nmol / L, 100nmol / L, 1000nmol / L by mixing different volumes of pentachlorophenol solutions. L, 10000nmol / L. Simultaneously mixed luminescence system: the concentration of ...

Embodiment 3

[0045] Be that 2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol etc. replace the pentachlorophenol in embodiment example 1 with the concentration of 10 μ M, other conditions such as luminol, h 2 o 2 , G-quadruplex / Hemin peroxide mimetic enzyme buffer solution and other chemiluminescence system conditions are the same as in Example 1, and the chemiluminescence intensity of the system with different chlorophenol compounds added is measured. See Table 2 for the CL intensity change value ΔCL of chemiluminescence.

[0046] Table 2 Changes in chemiluminescence intensity after interacting with different chlorophenol compounds

[0047]

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Abstract

The invention relates to the technical field of DNA biological sensing in analytic chemistry and optics and particularly discloses a biological sensing method for detecting chlorophenol pollutants on the basis of G-quadruplet / Hemin mimetic enzyme of peroxidase-hydrogen peroxide-luminol chemiluminescent system. The chemiluminescent method has the advantages of simplicity, fastness, low cost, few sample use amount, high sensitivity and the like. The method disclosed by the invention provides a new detection method for fast detection of the chlorophenol pollutants in the environment system, plays an important role in perfecting the existing detection technology of the chlorophenol pollutants, and simultaneously expands the application of the G-quadruplet / Hemin mimetic enzyme of peroxidase in the field of analytic chemistry.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry and optical DNA biosensing, in particular to a method for detecting chlorophenol pollutants by using a chemiluminescent method. Background technique [0002] Chlorophenols are a class of widely distributed toxic environmental pollutants. Chlorophenol compounds are widely used in the production of plastics, dyes, pesticides, herbicides and other industrial production processes, and are also used in wood preservation, fungicides, chlorinated pesticide intermediates and additives for adhesives, etc. An important reason for the wide distribution of chlorophenols. Chlorophenols can persist in the environmental system, have bioaccumulation, and have strong biological toxicity. Chlorophenols pollutants entering the body will cause harm to organisms—not only affecting and changing the immune system and nervous system and the normal regulatory function of the endocrine system, causing pathol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 梁刚靳欣欣满燕贾文珅潘立刚
Owner BEIJING RES CENT FOR AGRI STANDARDS & TESTING
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