Chemiluminescence intensifier

A chemiluminescence and enhancer technology, applied in the field of chemiluminescence analysis technology, can solve the problems of weak intensity and short duration, and achieve the effects of low cost, enhanced luminescence intensity, and prolonged duration

Inactive Publication Date: 2012-04-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, if no enhancer is used, the luminescence of the lum...

Method used

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  • Chemiluminescence intensifier
  • Chemiluminescence intensifier

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Experimental program
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Effect test

Embodiment 1

[0017] Weigh 10mMol hydroquinone and 1mMol 3,5-di-tert-butylsalicylic acid, and set the volume to 1L of 0.1Mol / L, pH 7.5 Tris-HCl buffer to obtain a concentration of 10mMol / L hydroquinone and 1mMol / L 3,5-di-tert-butyl salicylic acid as a chemiluminescent enhancer. Add 2.5 g of luminol to the chemiluminescence enhancer to make the concentration of luminol reach 2.5 mg / mL, and store the solution in a refrigerator at 4° C. as chemiluminescence substrate A solution. The chemiluminescent substrate B solution is 0.1Mol / L, pH 7.5 Tris-HCl buffer solution containing 0.4g / mL carbamide peroxide, and stored in a refrigerator at 4°C. Before use, mix chemiluminescence substrate A solution containing luminol, hydroquinone and 3,5-di-tert-butylsalicylic acid with chemiluminescence substrate B solution containing carbamide peroxide at a ratio of 1:1.

Embodiment 2

[0019] According to the chemiluminescent system of embodiment 1 (containing luminescent agent luminol, chemiluminescence enhancer and oxidizing agent carbamide peroxide), in addition to adding horseradish peroxidase, chemiluminescence reaction can occur, and the luminescence value and luminescence time can be controlled Detection was performed by a chemiluminescent detector. Test results such as figure 1 shown, from figure 1 It can be seen that the chemiluminescence enhancer composed of hydroquinone and 3,5-di-tert-butylsalicylic acid effectively enhanced the luminescence intensity of the luminol-carbamide peroxide chemiluminescence system and prolonged its luminescence duration, The stability of the luminous intensity is basically maintained within 2-150 minutes.

Embodiment 3

[0021] Weigh 1mMol hydroquinone and 0.1mMol 3,5-di-tert-butylsalicylic acid, and set the volume to 1L 5Mol / L, pH 10 Tris-HCl buffer solution to obtain a concentration of 1mMol / L hydroquinone and 0.1mMol / L3,5-di-tert-butyl salicylic acid as a chemiluminescent enhancer. Add 5 g of luminol to the chemiluminescence enhancer to make the concentration of luminol reach 5 mg / mL, and store the solution in a refrigerator at 4°C as chemiluminescence substrate A solution. The chemiluminescence substrate B solution is 5Mol / L, pH 10 Tris-HCl buffer solution containing carbamide peroxide 0.1g / mL, and stored in a refrigerator at 4°C. Before use, mix chemiluminescent substrate A solution containing luminol, hydroquinone and 3,5-di-tert-butylsalicylic acid with chemiluminescent substrate B solution containing carbamide peroxide at a ratio of 1:1.

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Abstract

The invention relates to a chemical luminescent reinforcing agent, which comprises hydroquinone, 3, 5-ditert-butyl salicylic acid and Tris-HCl buffer solution, wherein the dosage of the effective ingredient hydroquinone is between 1 and 100mMol/L, and the dosage of the 3, 5-ditert-butyl salicylic acid is between 0.1 and 50mMol/L, the ion concentration of the Tris-HCl buffer solution is between 0.1 and 5 Mol/L and pH value is between 7.5 and 10. The chemical luminescent reinforcing agent has the advantages that the chemical luminescent reinforcing agent has convenient use, low cost, remarkableimprovement on luminous intensity and luminous endurance time of a luminol chemical luminescent system, and can be widely applied to chemical luminescence detection for various biological macromolecules and biological micromolecules in clinical examination, biological research, agricultural and forest industries, animal husbandry and environmental science.

Description

technical field [0001] The invention relates to a chemiluminescence enhancer, which is used for improving the luminescence intensity and luminescence duration of a luminol chemiluminescence system, and is applied to chemiluminescence analysis technology. Background technique [0002] Chemiluminescence is the formation of excited state molecules in chemical reactions through the decomposition of high-energy intermediates. The excited state molecules are unstable, and when they return to the ground state, part of their energy is released in the form of luminescence. In 1977, Halman first applied purification chemiluminescence to immunoassay and established chemiluminescence immunoassay technology. Among modern microanalysis techniques, chemiluminescence immunoassay technology has the characteristics of high sensitivity, simplicity, speed, linear stability, safety and harmlessness. [0003] Luminol, Isoluminol and their derivatives are the first chemiluminescent substances use...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 陈峰徐晓婴卢永红卢庆鋆梁晓翠
Owner SHANGHAI JIAO TONG UNIV
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