Method for detecting ferritin based on nanogold catalytic chemiluminescence analysis

Abstract

The invention discloses a method for detecting ferritin based on nanogold catalytic chemiluminescence analysis. The method comprises the following steps that 1), a nanogold solution is prepared, 0.01% w / w of a HAuC14 solution of is heated to boiling, a sodium citrate solution of 1% is added under hard stirring, a heating source is removed after the mixed solution keeps boiling for fifteen minutes, the mixed solution is continuously stirred and cooled to the room temperature, and the obtained solution is stored in a refrigerator with the temperature of 4 DEG C for standby application; 2) nanogold is marked through ferritin antibodies, and the ferritin antibodies are utilized for modifying nanogold particles; 3), immunoreaction is carried out; 4), ferritin is detected through the chemiluminescence analysis, a nonogold / ferritin mixed solution modified by the ferritin antibodies after the immunoreaction is finished is moved into a chemiluminescence pool, a luminol-potassium periodate chemiluminescence reagent is injected into the solution, and the chemiluminescence intensity of the solution is measured and recorded through an IFFL-D chemiluminescencent analyzer to obtain the correlation between the chemiluminescence intensity and the ferritin to be measured.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular, the invention relates to a method for detecting ferritin by chemiluminescent analysis based on nano-gold catalysis. Background technique [0002] Ferritin is a kind of protein with high iron content widely present in animal, plant and microbial cells, which can participate in and maintain the balance of iron metabolism. Ferritin in the human body can avoid iron poisoning by storing excess iron in the body, and can also release iron to iron-demanding cells for the synthesis of iron-containing proteins or enzymes. Many people's diseases such as iron deficiency anemia, acute hepatitis and protein deficiency are related to the content of ferritin in serum, and even some cancer patients have abnormal ferritin in their bodies, which can be used as an auxiliary diagnosis for some cancers, so it is important for Accurate and rapid determination of ferritin is of great significance....

AI Technical Summary

Problems solved by technology

The above nanoparticle chemiluminescent metal immunoassay methods have certain defects: 1. The dissolution of nanometer metal ions is required, and the conditions for completely stripping out nanoparticles, especially noble metal nanoparticles, are relatively harsh and most of the solvents used are toxic.

The dissolution steps are cumbersome and time-consuming, and will bring high background signals, thereby reducing the sensitivity of the method; second, most of these analytical methods are heterogeneous, requiring multi-step labeling and elution, which makes it difficult to overcome reproducibility and precision The problem

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