Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)

A photochemiluminescence and immunoassay technology, which is applied in the field of serum-specific IgE photochemiluminescence immunoassay kits, can solve problems affecting the accuracy of detection results, and prevent steric hindrance effects and small biotin molecules. , the effect of simple operation

Inactive Publication Date: 2016-07-20
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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Problems solved by technology

The enzyme catalyzes the substrate to show high efficiency, which improves the sensitivity of the analysis method; however, the enzyme is a

Method used

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  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)
  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)
  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)

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Embodiment Construction

[0032] The present invention will be further illustrated below by specific examples.

[0033] Taking "egg-specific IgE detection" as an example for illustration, the specific implementation method is as follows:

[0034] Poultry eggs are common food allergens, and poultry egg allergy is a common food allergic reaction, especially in infants and young children. The main allergens of poultry eggs include: wherein ovomucoid (Ovomuciod, OM, Gald1, 28kDa, 11%), ovalbumin (Ovalbumin, OA, Gald2, 45kDa, 54%), ovotransferrin (Ovotransferrin, OT, Gald3, 77 kDa, 12%). This example uses "ovalbumin (Gald2)" as an example to illustrate the composition, preparation process, use method, result analysis, etc. of the diagnostic reagent.

[0035] (1) Main reagents

[0036] (1) Luminescent microsphere solution (coated with ovalbumin)

[0037] (2) Biotin biotin-labeled anti-human IgE antibody solution (commercially available)

[0038] (3) Photosensitive microsphere solution (streptavidin) (co...

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Abstract

The invention provides a light-activating chemiluminescence immunity analysis kit for serum specific IgE (immunoglobulin E). The kit adopts a luminous microsphere coated with a specific single-component allergen (prepared by naturally purification or recombination), a biotin-labeled anti-human-IgE antibody and a light-sensitive microsphere precoated with streptavidin to form detecting reagents. Firstly, to-be-detected serum, the luminous microsphere coated the allergen and the a biotin-labeled anti-human-IgE antibody are incubated together, and the specific IgE antibody in the to-be-detected serum is bound with an antigen molecule on the surface of the luminous microsphere and the biotin-labeled anti-human-IgE antibody respectively; secondly, the light-sensitive microsphere precoated with streptavidin is added for continuous incubation, and is bridged with the luminous microsphere through the binding of biotin and streptavidin; thirdly, a light-activating chemiluminescence analyzing instrument is used for detecting the strength of light signals. The kit has various advantages of being easy and quick to operate, facilitating automation and quantitative analysis, and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a serum-specific IgE (allergen) photo-induced chemiluminescence immunoassay kit. Background technique [0002] At present, the diagnosis of allergic diseases mainly relies on medical history, in vivo skin prick test and in vitro serum specific IgE detection. Among them, serum specific IgE has high safety performance, is not affected by clinical medication, and can detect many kinds of allergens at the same time. advantage. Therefore, quantitative or semi-quantitative detection of serum-specific IgE has become a routine laboratory index for the confirmation of allergens in allergic diseases. [0003] First, the original method for serum-specific IgE detection is the "radioallergic allergen adsorption test (RAST)". In this method, the corresponding allergen solution (mixed components) is coated on the surface of the solid phase material (nitrocellulose membrane, NC membra...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N21/76
CPCG01N33/6854G01N21/76G01N33/54313
Inventor 李会强赵卫国闫娟娟张向辉佘钿田
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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