Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)

A photochemiluminescence and immunoassay technology, which is applied in the field of serum-specific IgE photochemiluminescence immunoassay kits, can solve problems affecting the accuracy of detection results, and prevent steric hindrance effects and small biotin molecules. , the effect of simple operation

Inactive Publication Date: 2016-07-20
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
View PDF5 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme catalyzes the substrate to show high efficiency, which improves the sensitivity of the analysis method; however, the enzyme is a biologically active protein, and many factors will affect the activity of the enzyme, which will also affect the accuracy of the test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)
  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)
  • Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be further illustrated below by specific examples.

[0033] Taking "egg-specific IgE detection" as an example for illustration, the specific implementation method is as follows:

[0034] Poultry eggs are common food allergens, and poultry egg allergy is a common food allergic reaction, especially in infants and young children. The main allergens of poultry eggs include: wherein ovomucoid (Ovomuciod, OM, Gald1, 28kDa, 11%), ovalbumin (Ovalbumin, OA, Gald2, 45kDa, 54%), ovotransferrin (Ovotransferrin, OT, Gald3, 77 kDa, 12%). This example uses "ovalbumin (Gald2)" as an example to illustrate the composition, preparation process, use method, result analysis, etc. of the diagnostic reagent.

[0035] (1) Main reagents

[0036] (1) Luminescent microsphere solution (coated with ovalbumin)

[0037] (2) Biotin biotin-labeled anti-human IgE antibody solution (commercially available)

[0038] (3) Photosensitive microsphere solution (streptavidin) (co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a light-activating chemiluminescence immunity analysis kit for serum specific IgE (immunoglobulin E). The kit adopts a luminous microsphere coated with a specific single-component allergen (prepared by naturally purification or recombination), a biotin-labeled anti-human-IgE antibody and a light-sensitive microsphere precoated with streptavidin to form detecting reagents. Firstly, to-be-detected serum, the luminous microsphere coated the allergen and the a biotin-labeled anti-human-IgE antibody are incubated together, and the specific IgE antibody in the to-be-detected serum is bound with an antigen molecule on the surface of the luminous microsphere and the biotin-labeled anti-human-IgE antibody respectively; secondly, the light-sensitive microsphere precoated with streptavidin is added for continuous incubation, and is bridged with the luminous microsphere through the binding of biotin and streptavidin; thirdly, a light-activating chemiluminescence analyzing instrument is used for detecting the strength of light signals. The kit has various advantages of being easy and quick to operate, facilitating automation and quantitative analysis, and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a serum-specific IgE (allergen) photo-induced chemiluminescence immunoassay kit. Background technique [0002] At present, the diagnosis of allergic diseases mainly relies on medical history, in vivo skin prick test and in vitro serum specific IgE detection. Among them, serum specific IgE has high safety performance, is not affected by clinical medication, and can detect many kinds of allergens at the same time. advantage. Therefore, quantitative or semi-quantitative detection of serum-specific IgE has become a routine laboratory index for the confirmation of allergens in allergic diseases. [0003] First, the original method for serum-specific IgE detection is the "radioallergic allergen adsorption test (RAST)". In this method, the corresponding allergen solution (mixed components) is coated on the surface of the solid phase material (nitrocellulose membrane, NC membra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/543G01N21/76
CPCG01N33/6854G01N21/76G01N33/54313
Inventor 李会强赵卫国闫娟娟张向辉佘钿田
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com