Magnetic particle chemiluminescence assay kit for detecting angiotensin II

Abstract

The invention discloses a magnetic particle chemiluminescence assay kit for detecting angiotensin II. The kit comprises magnetic particle suspension coupled with anti-rabbit angiotensin II antibody, a calibrator, angiotensin II antigen coupled with biotin, horse radish peroxidase labeled streptavidin, enhanced chemiluminescent A, enhanced chemiluminescent B and lotion. The invention has the advantages that: the kit can be operated conveniently and automatically, a detection result is high in repeatability and accuracy, detection time is short, the stability can be kept for twelve months, and radio contamination can be avoided. During testing, a sample is introduced by a one-step method, namely the magnetic particle suspension, a sample to be tested, the angiotensin II antigen coupled with biotin, and the horse radish peroxidase labeled streptavidin which take part in reaction are added into a reaction cup in one operation step, so operation steps and reaction time are reduced, and errors brought in an operation process are reduced.

Description

technical field [0001] The invention relates to the field of medical diagnostic reagents, in particular to a kit for detecting the content of angiotensin II by using chemiluminescence technology combined with magnetic particle coupling and separation technology. Background technique [0002] Angiotensin, a polypeptide that constricts blood vessels and raises blood pressure, is part of the renin-angiotensin-aldosterone system. Many antihypertensive drugs target angiotensin. Angiotensin also stimulates the adrenal cortex to secrete aldosterone. Aldosterone promotes sodium retention in distal nephrons and can also raise blood pressure. [0003] Angiotensin II is a direct-acting substance of the renin-angiotensin-aldosterone system regulating blood pressure. At the same time, angiotensin II can also act as a growth-promoting factor to directly promote the proliferation of vascular endothelial cells. Recent studies have found that angiotensin II is directly related to vascula...

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Problems solved by technology

[0006] At present, the method for detecting angiotensin II is mainly based on radioimmunoassay technology, which is used to measure angiotensin II, but its sensitivity and anti-interference ability are seriously insufficient. It is not easy to control, resulting in poor repeatability and large variation of the measurement results. In addition, the radioimmunoassay method has the defects of long detection time and poor stability, and there is also the problem of radioactive source contamination

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