Magnetic particle chemiluminescence assay kit for detecting angiotensin II

A chemiluminescence reagent, angiotensin technology, applied in chemiluminescence/bioluminescence, biological testing, analysis through chemical reaction of materials, etc., can solve problems such as poor stability, pollution of radioactive sources, large variation, etc., to achieve good results The effect of repeatability and accuracy, no radioactive contamination, and simplicity

Inactive Publication Date: 2012-07-04
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

[0006] At present, the method for detecting angiotensin II is mainly based on radioimmunoassay technology, which is used to measure angiotensin II, but its sensitivity and anti-interference ability are seriously insufficient. It is not easy to cont

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  • Magnetic particle chemiluminescence assay kit for detecting angiotensin II
  • Magnetic particle chemiluminescence assay kit for detecting angiotensin II

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Embodiment Construction

[0017] The magnetic particle chemiluminescence reagent kit for detecting angiotensin II according to the present invention includes magnetic particle suspension coupled with rabbit anti-angiotensin II antibody, calibrator, and biotin-coupled angiotensin II Antigen, horseradish peroxidase-labeled streptavidin, chemiluminescent substrate A, chemiluminescent substrate B, and washing solution.

[0018] The magnetic particles coupled with rabbit anti-angiotensin II antibody were first coupled with goat anti-rabbit IgG, and then indirect immunoconjugated rabbit anti-angiotensin II antibody, using the aminoglutaraldehyde method; calibrator with 0.05M Tris- NaCl and 2% casein make up pH 7.4 buffer as the matrix and add pure angiotensin II to prepare; biotin-conjugated angiotensin II antigen uses 0.01M PBS and 2% BSA as the buffer matrix Diluted to a working concentration of 1:10000; horseradish peroxidase-labeled streptavidin was diluted to a working concentration of 1:6000 with 0...

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Abstract

The invention discloses a magnetic particle chemiluminescence assay kit for detecting angiotensin II. The kit comprises magnetic particle suspension coupled with anti-rabbit angiotensin II antibody, a calibrator, angiotensin II antigen coupled with biotin, horse radish peroxidase labeled streptavidin, enhanced chemiluminescent A, enhanced chemiluminescent B and lotion. The invention has the advantages that: the kit can be operated conveniently and automatically, a detection result is high in repeatability and accuracy, detection time is short, the stability can be kept for twelve months, and radio contamination can be avoided. During testing, a sample is introduced by a one-step method, namely the magnetic particle suspension, a sample to be tested, the angiotensin II antigen coupled with biotin, and the horse radish peroxidase labeled streptavidin which take part in reaction are added into a reaction cup in one operation step, so operation steps and reaction time are reduced, and errors brought in an operation process are reduced.

Description

technical field [0001] The invention relates to the field of medical diagnostic reagents, in particular to a kit for detecting the content of angiotensin II by using chemiluminescence technology combined with magnetic particle coupling and separation technology. Background technique [0002] Angiotensin, a polypeptide that constricts blood vessels and raises blood pressure, is part of the renin-angiotensin-aldosterone system. Many antihypertensive drugs target angiotensin. Angiotensin also stimulates the adrenal cortex to secrete aldosterone. Aldosterone promotes sodium retention in distal nephrons and can also raise blood pressure. [0003] Angiotensin II is a direct-acting substance of the renin-angiotensin-aldosterone system regulating blood pressure. At the same time, angiotensin II can also act as a growth-promoting factor to directly promote the proliferation of vascular endothelial cells. Recent studies have found that angiotensin II is directly related to vascula...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/52
Inventor 吴学炜赵鹏陈科李文娟王敏付光宇秦东春郑立运陆莹马建军渠海刘功成
Owner AUTOBIO DIAGNOSTICS CO LTD
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