Preparation method of voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence

Abstract

The invention discloses a preparation method of a voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence. The preparation method comprises the following steps of self-preparing a transformer and a multiplexer switch, preparing a multichannel printing electrode on paper, preparing a molecular imprinting (MIPs) sol of antibiotic residues, preparing carbon dots and silicon dioxide pellets coated by the carbon dots, preparing a graphene nano-material, and modifying the surface of the multichannel printing electrode on paper by the graphene nano-material, the silicon dioxide pellets coated by the carbon dots and the MIPs sol by an electrode surface modification technology. The preparation method also comprises the following steps that through the modified multichannel printing electrode, a chemiluminescent analyzer, the transformer and the multiplexer switch, antibiotic residues in sample extract are detected. The voltage-controllable multiplex multichannel sensing paper chip has strong singularity, high sensitivity reaching the ng grade and a low cost, can be operated fast and simply, and realizes automatic reactions and result recording by apparatuses.

Description

technical field [0001] The present invention relates to the technical field of antibiotic residue detection, more specifically to the preparation of a multi-channel sensor paper chip with molecularly imprinted electroluminescence detection of antibiotic residue with controllable voltage and multiplexing. Method for detecting antibiotic residues in milk, meat and egg products by electroluminescence sensor. Background technique [0002] Antibiotics are secondary metabolites of microorganisms that can kill or inhibit other microorganisms in the metabolic process. Since the 1950s, they have been widely used in the livestock and poultry industry. Their main uses include animal disease treatment, animal growth Regulators and improve feed utilization efficiency. Antibiotics, as drugs and non-drugs, play an important role in the development of livestock and poultry farming, and their use in many countries is also increasing. The use of antibiotics has made a considerable contribut...

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Problems solved by technology

[0006]2. UV-Vis spectrophotometry and fluorescence method have the advantages of simple operation, low cost, and wide linear range, but their sensitivity and selectivity are not high

[0007]3. The sensitivity of liquid chromatography-mass spectrometry is relatively high, but the instrument is expensive and the operation is relatively complicated

[0008]4. Enzyme-linked immunoassay has high sensitivity, but the operation steps are more complicated, but the reagents are expensive and the cost is relatively high

[0009]5. Microbial method has the advantages of low cost and time saving, but it is generally only used in screening experiments

[0010] For the detection and analysis of antibiotic residues in the above methods, there are generally high detection costs, false positive results, complicated detection process, high cost, large amount of reagents, It is not suitable for on-site rapid detection, and the interference of coexisting substances is large, so it cannot meet the needs of actual detection

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