4 results about "Thyroglobulin" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to a drug-loaded vesicle for treating thyroid cancer as well as a preparation method and application of the drug-loaded vesicle. The drug-loaded vesicle comprises a vesicle, wherein a cavity of the vesicle is loaded with radioactive iodine; the membrane surface of the vesicle is modified with: a) a first single-chain antibody fragment capable of specifically binding to thyroglobulin; according to the present invention, after the intravenous injection, the drug-loaded vesicle can be enriched in the thyroid cancer lesion area of the RAIR-DTC patient, such that the cancer cell proliferation is inhibited while the radioactive iodine is continuously released so as to provide the long-term treatment effect;

This invention relates to the field of immunoassay technology, and more specifically, to an antibody or antigen-binding fragment thereof capable of binding to thyroglobulin. The antibody or antigen-binding fragment thereof provided by this invention exhibits excellent affinity and sensitivity for thyroglobulin.

The invention provides a colloidal gold immunochromatography test strip detection method for rapidly detecting fomesafen, which comprises the following steps of: preparing fomesafen hapten and complete antigen: introducing carboxyl at a sulfonylamino part in a fomesafen structure to prepare immune hapten; coupling the immune hapten with thyroglobulin through a carbodiimide method to prepare an immunogen; preparation of a monoclonal antibody: immunizing a mouse with the obtained immunogen and preparing the fomesafen monoclonal antibody through a hybridoma technology; and preparation of the colloidal gold immunochromatography test strip: spraying the prepared coating antigen on a nitrocellulose membrane to serve as a detection line, and spraying a goat-anti-mouse secondary antibody on the nitrocellulose membrane to serve as a quality control line. The elimination line value of the test strip for detecting fomesafen residues is 16ng / mL. According to the method, the fomesafen residue in the field sample can be rapidly detected, so that the method has important guiding significance on planting of next-stubble crops.

The invention belongs to the technical field of PRRS (porcine reproductive and respiratory syndrome) virus particle antigen quantitative analysis, and particularly discloses a method for quantitatively detecting PRRS virus particles and application of the method. According to the method, a sucrose density gradient ultracentrifugation method is combined with a high performance liquid chromatograph to quantitatively detect the PRRS virus particles; comprising the following steps: firstly, preparing a 15-45% sucrose gradient by using an automatic gradient mixer, slowly adding a PRRS virus liquid sample at the top of the sucrose gradient, and horizontally and centrifugally separating PRRS virus particles; connecting the sucrose gradient solution with a pumping system of a high performance liquid chromatograph, continuously detecting optical signals of the sucrose gradient solution through an ultraviolet and laser detector, and integrating a chromatographic peak to obtain a peak area; and calculating the content of the PRRS virus particles according to the regression equation of the ultraviolet absorption peak area and the protein concentration of the thyroglobulin standard substance. The method has better accuracy, repeatability and stability, and is suitable for measuring the antigen content of the PRRS virus particles.