8 results about "Thyroglobulin" patented technology

The application relates to a thyroglobulin detection kit and a detection method, a sample puncture detection needle and application. The kit comprises a sample diluent and a sample puncture detection needle; a first coated carrier and a second coated carrier are sequentially arranged in the needle barrel along the axial direction of the needle barrel; the first coated carrier and the second coated carrier are matched with the gap of the needle barrel; the first coated carrier is close to the end of the needle head, the outer side of the first coated carrier is coated with a material capable of specifically combining with an antibody or an antigen of a human species; the second coated carrier is arranged at the end away from the needle head, and the outer side of the second coated carrier is coated with a material capable of specifically combining with an immune complex; a colorimetric card is arranged on the outer wall of the needle barrel. The technical problem solved is how to realize on-site rapid detection of the antibody or antigen concentration in the thyroglobulin, and overcome the defects that the prior art cannot meet the instantaneity and on-site nature of the operation.

The invention belongs to the technical field of biological medicines, and particularly relates to a drug-loaded vesicle for treating thyroid cancer as well as a preparation method and application of the drug-loaded vesicle. The drug-loaded vesicle comprises a vesicle, wherein a cavity of the vesicle is loaded with radioactive iodine; the membrane surface of the vesicle is modified with: a) a first single-chain antibody fragment capable of specifically binding to thyroglobulin; according to the present invention, after the intravenous injection, the drug-loaded vesicle can be enriched in the thyroid cancer lesion area of the RAIR-DTC patient, such that the cancer cell proliferation is inhibited while the radioactive iodine is continuously released so as to provide the long-term treatment effect;

This invention relates to the field of immunoassay technology, and more specifically, to an antibody or antigen-binding fragment thereof capable of binding to thyroglobulin. The antibody or antigen-binding fragment thereof provided by this invention exhibits excellent affinity and sensitivity for thyroglobulin.

The invention relates to an application of a traditional Chinese medicine composition in preparing a medicine for treating thyroiditis, which is characterized in that the traditional Chinese medicine composition is prepared from astragalus membranaceus, dried ginger, salted morinda officinalis, cortex dictamni, calcined oyster shell, schisandra chinensis and divaricate saposhnikovia root. The composition can obviously improve the thyroid functions of mice and rats, obviously reduce the content of inflammatory factors TNF-alpha, IL-6 and IL-12 in serum of the mice, obviously increase the level of TGF-beta in serum of the rats and reduce the level of IL-17, and has a good treatment effect on autoimmune thyroiditis induced by combination of thyroglobulin and high-iodine water.

The utility model provides a detection kit for thyroglobulin. The detection kit comprises a fixing assembly and a detection test tube, the fixing assembly comprises a box body, a containing cavity, a sliding groove, a sliding block, a plate body, a telescopic rod, a damping spring, a clamping groove and a clamping jaw. By means of the arrangement of the clamping jaws and the clamping grooves, the detection test tubes can be fixed in the containing cavity, when the detection test tubes need to be taken out of the containing cavity, the detection test tubes are pressed downwards, the damping springs are squeezed to be shrunk, and the detection test tubes can be taken out of the containing cavity, so that the detection test tubes can be taken out of the containing cavity, and the detection test tubes can be taken out of the containing cavity. The detection test tube moves downwards, the clamping jaws are extruded out of the clamping grooves and are stored in the grooves, the detection test tube is rotated at the moment, when the detection test tube moves upwards, the clamping jaws are always extruded to be located in the grooves, the detection test tube is loosened at the moment, the damping springs are reset, and the damping springs push the plate body to drive the detection test tube to move upwards; therefore, workers can conveniently take out the detection test tubes from the box body.

The invention provides colloidal gold immune test paper for detecting thyroglobulin and a preparation method of the colloidal gold immune test paper. The colloidal gold immune test paper for detecting thyroglobulin comprises a sample pad, a gold-labeled antibody binding pad, a nitrocellulose membrane and absorbent paper which are sequentially and partially spliced and fixed on a PVC (Polyvinyl Chloride) bottom plate, wherein the gold-labeled antibody combination pad is coated with a mouse anti-human thyroglobulin monoclonal antibody labeled by colloidal gold; a detection line and a quality control line which are separated from each other are arranged on the nitrocellulose membrane, the detection line is located at the 1 / 3 position of the nitrocellulose membrane, and the quality control line is located at the 2 / 3 position of the nitrocellulose membrane; the detection line is coated with an anti-human thyroglobulin specific antibody; and the quality control line is coated with an anti-mouse IgG antibody. The test paper does not need specific equipment and professional technicians, the detection time is short, the result can be interpreted in only about ten minutes, the manpower, material resources and time cost are greatly saved, and the test paper has very high practical value and economic benefit.

The invention provides a colloidal gold immunochromatography test strip detection method for rapidly detecting fomesafen, which comprises the following steps of: preparing fomesafen hapten and complete antigen: introducing carboxyl at a sulfonylamino part in a fomesafen structure to prepare immune hapten; coupling the immune hapten with thyroglobulin through a carbodiimide method to prepare an immunogen; preparation of a monoclonal antibody: immunizing a mouse with the obtained immunogen and preparing the fomesafen monoclonal antibody through a hybridoma technology; and preparation of the colloidal gold immunochromatography test strip: spraying the prepared coating antigen on a nitrocellulose membrane to serve as a detection line, and spraying a goat-anti-mouse secondary antibody on the nitrocellulose membrane to serve as a quality control line. The elimination line value of the test strip for detecting fomesafen residues is 16ng / mL. According to the method, the fomesafen residue in the field sample can be rapidly detected, so that the method has important guiding significance on planting of next-stubble crops.

The invention belongs to the technical field of PRRS (porcine reproductive and respiratory syndrome) virus particle antigen quantitative analysis, and particularly discloses a method for quantitatively detecting PRRS virus particles and application of the method. According to the method, a sucrose density gradient ultracentrifugation method is combined with a high performance liquid chromatograph to quantitatively detect the PRRS virus particles; comprising the following steps: firstly, preparing a 15-45% sucrose gradient by using an automatic gradient mixer, slowly adding a PRRS virus liquid sample at the top of the sucrose gradient, and horizontally and centrifugally separating PRRS virus particles; connecting the sucrose gradient solution with a pumping system of a high performance liquid chromatograph, continuously detecting optical signals of the sucrose gradient solution through an ultraviolet and laser detector, and integrating a chromatographic peak to obtain a peak area; and calculating the content of the PRRS virus particles according to the regression equation of the ultraviolet absorption peak area and the protein concentration of the thyroglobulin standard substance. The method has better accuracy, repeatability and stability, and is suitable for measuring the antigen content of the PRRS virus particles.