19 results about "Thyroglobulin" patented technology

The present invention relates to the technical field of tumor treatment, and specifically provides an anti-tumor composite dressing and a preparation method thereof. The anti-tumor composite dressing includes: the traditional Chinese medicine extract and beeswax with a mass 0.05 times that of the traditional Chinese medicine extract. The anti-tumor composite dressing of the present invention mainly promotes blood circulation to dissipate stasis and secondarily activates the heart and promotes blood circulation, promotes the immune cycle, delays or even alleviates thyroid tumors, and finally achieves the goal of not undergoing surgery and maintaining a relatively normal thyroglobulin content for a long time.

The application relates to a thyroglobulin detection kit and a detection method, a sample puncture detection needle and application. The kit comprises a sample diluent and a sample puncture detection needle; a first coated carrier and a second coated carrier are sequentially arranged in the needle barrel along the axial direction of the needle barrel; the first coated carrier and the second coated carrier are matched with the gap of the needle barrel; the first coated carrier is close to the end of the needle head, the outer side of the first coated carrier is coated with a material capable of specifically combining with an antibody or an antigen of a human species; the second coated carrier is arranged at the end away from the needle head, and the outer side of the second coated carrier is coated with a material capable of specifically combining with an immune complex; a colorimetric card is arranged on the outer wall of the needle barrel. The technical problem solved is how to realize on-site rapid detection of the antibody or antigen concentration in the thyroglobulin, and overcome the defects that the prior art cannot meet the instantaneity and on-site nature of the operation.

The invention belongs to the technical field of biological medicines, and particularly relates to a drug-loaded vesicle for treating thyroid cancer as well as a preparation method and application of the drug-loaded vesicle. The drug-loaded vesicle comprises a vesicle, wherein a cavity of the vesicle is loaded with radioactive iodine; the membrane surface of the vesicle is modified with: a) a first single-chain antibody fragment capable of specifically binding to thyroglobulin; according to the present invention, after the intravenous injection, the drug-loaded vesicle can be enriched in the thyroid cancer lesion area of the RAIR-DTC patient, such that the cancer cell proliferation is inhibited while the radioactive iodine is continuously released so as to provide the long-term treatment effect;

The invention relates to the related technical field of thyroglobulin detection, in particular to a thyroglobulin chemiluminescence detection kit and a detection method thereof.The thyroglobulin chemiluminescence detection kit comprises a kit body structure, the kit body structure is composed of an upper shell and a lower shell which are detachably connected in a sealed connection mode, and a dripping detection hole is formed in the upper shell; the thickening pad and the analysis structure are located in the box body structure, and a gap exists between the thickening pad and the analysis structure; the blocking assembly is installed on the upper shell, a connecting pad for conducting the thickening pad and the analysis structure is installed at the end, arranged in the box body structure, of the blocking assembly, and when the blocking assembly is controlled to release the blocking state of the drop measuring hole, the blocking assembly is controlled to release the blocking state of the drop measuring hole. When the kit is used, the connecting pad can be driven to move downwards to abut against the thickening pad and the analysis structure, and it is guaranteed that the interior of the kit body structure is always kept closed before the kit is started, so that external substances cannot enter the kit body structure through the dropping detection hole.

This invention relates to the field of immunoassay technology, and more specifically, to an antibody or antigen-binding fragment thereof capable of binding to thyroglobulin. The antibody or antigen-binding fragment thereof provided by this invention exhibits excellent affinity and sensitivity for thyroglobulin.

Provided is a novel method that enables accurate measurement of thyroglobulin content in a sample even when the sample is positive for an anti-thyroglobulin antibody. The method is a method of measuring thyroglobulin by immunoassay in a sample isolated from the body, including a pretreatment step of treating the sample isolated from the body with a reducing agent, wherein a monoclonal antibody or an antigen-binding fragment thereof whose corresponding antigen is thyroglobulin treated with the reducing agent is used for the immunoassay.

Disclosed is a synthetic T cell receptor (TCR) having antigenic specificity for an HLA-A2-restricted epitope of thyroglobulin (TG), TG470-478. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, and populations of cells are also provided. Antibodies, or an antigen binding portion thereof, and pharmaceutical compositions relating to the TCRs of the invention are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

The invention relates to an application of a traditional Chinese medicine composition in preparing a medicine for treating thyroiditis, which is characterized in that the traditional Chinese medicine composition is prepared from astragalus membranaceus, dried ginger, salted morinda officinalis, cortex dictamni, calcined oyster shell, schisandra chinensis and divaricate saposhnikovia root. The composition can obviously improve the thyroid functions of mice and rats, obviously reduce the content of inflammatory factors TNF-alpha, IL-6 and IL-12 in serum of the mice, obviously increase the level of TGF-beta in serum of the rats and reduce the level of IL-17, and has a good treatment effect on autoimmune thyroiditis induced by combination of thyroglobulin and high-iodine water.

The invention discloses a medicine for preventing hashimoto thyroiditis as well as a preparation method and application thereof, the medicine is a 10 [mu] g / [mu] l sterile pig thyroid freeze-dried powder solution, and the active ingredients of the medicine are thyroglobulin and thyroid peroxidase. The preparation method comprises the following steps: separating thyroid tissues from necks of just slaughtered pigs; removing connective tissues in the thyroid tissues in an ice bath, and grinding the thyroid tissues from which the connective tissues are removed by using a grinding machine to obtain a thyroid extract; freezing the thyroid extract overnight and placing the thyroid extract in liquid nitrogen for a preset time so as to pre-treat the thyroid extract; and carrying out freeze-drying treatment on the pretreated thyroid extract to obtain pig thyroid freeze-dried powder, and carrying out cryopreservation on the pig thyroid freeze-dried powder. Immune tolerance can be induced by oral administration or subcutaneous injection of the pig thyroid freeze-dried powder prepared by the invention for a newborn CBA / J mouse, so that autoimmune thyroiditis after adult can be prevented.

The utility model provides a detection kit for thyroglobulin. The detection kit comprises a fixing assembly and a detection test tube, the fixing assembly comprises a box body, a containing cavity, a sliding groove, a sliding block, a plate body, a telescopic rod, a damping spring, a clamping groove and a clamping jaw. By means of the arrangement of the clamping jaws and the clamping grooves, the detection test tubes can be fixed in the containing cavity, when the detection test tubes need to be taken out of the containing cavity, the detection test tubes are pressed downwards, the damping springs are squeezed to be shrunk, and the detection test tubes can be taken out of the containing cavity, so that the detection test tubes can be taken out of the containing cavity, and the detection test tubes can be taken out of the containing cavity. The detection test tube moves downwards, the clamping jaws are extruded out of the clamping grooves and are stored in the grooves, the detection test tube is rotated at the moment, when the detection test tube moves upwards, the clamping jaws are always extruded to be located in the grooves, the detection test tube is loosened at the moment, the damping springs are reset, and the damping springs push the plate body to drive the detection test tube to move upwards; therefore, workers can conveniently take out the detection test tubes from the box body.

The invention relates to the technical field of biomedicine, in particular to a thyroglobulin detection method and a thyroglobulin detection kit. The detection method comprises the following steps: providing a to-be-detected sample, and pretreating the to-be-detected sample to obtain a pretreated sample; detecting the thyroglobulin in the pretreated sample; wherein the pretreatment comprises the following steps: mixing the sample to be detected with an acidic reagent, and incubating; the acidic reagent comprises organic acid or salt thereof. The invention also provides a corresponding kit. By applying the detection method or the kit provided by the invention, interference of the thyroglobulin antibody TgAb in a sample on a detection result can be avoided, and accurate quantification of thyroglobulin is realized.

The invention provides colloidal gold immune test paper for detecting thyroglobulin and a preparation method of the colloidal gold immune test paper. The colloidal gold immune test paper for detecting thyroglobulin comprises a sample pad, a gold-labeled antibody binding pad, a nitrocellulose membrane and absorbent paper which are sequentially and partially spliced and fixed on a PVC (Polyvinyl Chloride) bottom plate, wherein the gold-labeled antibody combination pad is coated with a mouse anti-human thyroglobulin monoclonal antibody labeled by colloidal gold; a detection line and a quality control line which are separated from each other are arranged on the nitrocellulose membrane, the detection line is located at the 1 / 3 position of the nitrocellulose membrane, and the quality control line is located at the 2 / 3 position of the nitrocellulose membrane; the detection line is coated with an anti-human thyroglobulin specific antibody; and the quality control line is coated with an anti-mouse IgG antibody. The test paper does not need specific equipment and professional technicians, the detection time is short, the result can be interpreted in only about ten minutes, the manpower, material resources and time cost are greatly saved, and the test paper has very high practical value and economic benefit.

The present application relates to anti-thyroglobulin antibodies, kits and uses thereof. Specifically, the present application provides anti-thyroglobulin specific antibodies, kits containing the antibodies, as well as uses thereof in detecting the presence or level of thyroglobulin and / or thyroglobulin autoantibodies in a sample.

A composition comprising a collection of solid phase substrates coated with one or more homogeneous populations of binding agents, wherein each homogeneous population of binding agents specifically binds to an antibody directed against a single antigen selected from the group consisting of: dexamethasone-induced transcript (DEXI), CXC motif chemokine ligand 11 (CXCL11), cytokeratin 18 (KRT18), cytokeratin 8 (KRT8), tubulin, including tubulin alpha 1 b (also referred to as TUBa1b or TUBA1B), latrophilin 1 (LPHN1), colony stimulating factor 2 (CSF2), signal transducer and activator of transcription 6 (STAT6), lectin galactoside-binding soluble 3 (lectin galactoside-binding soluble 3“;LGALS3), SHC adaptor protein 3 (SHC3), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glutathione S-transferase theta-1 (GSTT1), phospholipase A2 receptor 1 (PLA2R1), interleukin 8 (IL-8), lectin galactoside-binding soluble 8 (LGALS8), small nuclear ribonucleoprotein polypeptide N (SNPRN), myosin, peroxisomal trans-2-enoyl-CoA reductase (PECR), vimentin (VIM), ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide (ATP5B), collagen II, prelamin A / C (LMNA), small nuclear ribonucleoprotein polypeptide B (SNRPB2), fibronectin 1 (FN1), nephrosis 1, congenital Finnish type nephrosis 1 (NPHS1), thyroglobulin (TG) and vinculin (VCL).;

The invention discloses a thyroid protein detection probe based on aptamer fluorescence enhancement and application of the thyroid protein detection probe, and belongs to the technical field of biological medicine. The thyroglobulin aptamer probe Tg-Apt is obtained by modifying a fluorophore in a specific area, and the aptamer probe has high affinity and specificity and can be specifically combined with Tg in a complex biological sample. The aptamer probe Tg-Apt and a to-be-detected sample are subjected to a mixed reaction, and when Tg-Apt is combined with thyroglobulin, a fluorescence enhancement phenomenon occurs to realize detection of thyroglobulin, so that rapid identification of thyroid cancer or thyroid cancer metastasis is realized. The detection method disclosed by the invention has the characteristics of high sensitivity, high specificity and simplicity, convenience and rapidness in operation, provides an effective tool for diagnosis and treatment of thyroid cancer, and has a good clinical application prospect.

This application provides an antibody or antigen-binding fragment thereof that binds to thyroglobulin, including: a heavy chain variable region having: CDR-H1 having an amino acid sequence selected from SEQ ID NOs: 1, 7, 13, 19, 25, 31, and variants thereof, CDR-1-12 having an amino acid sequence selected from SEQ ID NOs: 2, 8, 14, 20, 26, 32, and variants thereof, and CDR-H3 having an amino acid sequence selected from SEQ ID NOs: 3, 9, 15, 21, 27, 33, and variants thereof; and a light chain variable region having: CDR-L1 having an amino acid sequence selected from SEQ ID NOs: 4, 10, 16, 22, 28, 34, and variants thereof, CDR-L2 having an amino acid sequence selected from SEQ ID NOs: 5, 11, 17, 23, 29, 35, and variants thereof, and CDR-L3 having an amino acid sequence selected from SEQ ID NOs: 6, 12, 18, 24, 30, 36, and variants thereof.

The invention provides a colloidal gold immunochromatography test strip detection method for rapidly detecting fomesafen, which comprises the following steps of: preparing fomesafen hapten and complete antigen: introducing carboxyl at a sulfonylamino part in a fomesafen structure to prepare immune hapten; coupling the immune hapten with thyroglobulin through a carbodiimide method to prepare an immunogen; preparation of a monoclonal antibody: immunizing a mouse with the obtained immunogen and preparing the fomesafen monoclonal antibody through a hybridoma technology; and preparation of the colloidal gold immunochromatography test strip: spraying the prepared coating antigen on a nitrocellulose membrane to serve as a detection line, and spraying a goat-anti-mouse secondary antibody on the nitrocellulose membrane to serve as a quality control line. The elimination line value of the test strip for detecting fomesafen residues is 16ng / mL. According to the method, the fomesafen residue in the field sample can be rapidly detected, so that the method has important guiding significance on planting of next-stubble crops.

The present invention discloses a method for determining foot-and-mouth disease virus-like particles by sucrose density gradient ultracentrifugation, and relates to the technical field of quantitative analysis of foot-and-mouth disease virus-like particles antigen. The present invention specifically comprises the following steps: preparing a 20%-45% sucrose gradient with an automatic gradient mixer, placing 500µl foot-and-mouth disease virus-like particles on the top of the sucrose gradient; separating virus-like particles by centrifugation at 38000rpm for 3.5h at 10°C; connecting an ultracentrifuge tube to a high performance liquid chromatograph, using the pump head of the chromatograph as the driving force, and detecting a flow rate of 0.28 ml / min; continuously detecting the optical signal of the sucrose gradient solution by using an ultraviolet and laser detector, and analyzing the peak area of ​​the sample by a computer software system; calculating the content of virus-like particles according to the regression equation of the ultraviolet absorption peak area and protein concentration of a thyroglobulin standard. The present invention can be used for determining the assembly efficiency of foot-and-mouth disease virus-like particles and monitoring the antigen content of a variety of foot-and-mouth disease virus-like particle vaccines, and indirectly evaluating the efficacy of the vaccine.

The invention belongs to the technical field of PRRS (porcine reproductive and respiratory syndrome) virus particle antigen quantitative analysis, and particularly discloses a method for quantitatively detecting PRRS virus particles and application of the method. According to the method, a sucrose density gradient ultracentrifugation method is combined with a high performance liquid chromatograph to quantitatively detect the PRRS virus particles; comprising the following steps: firstly, preparing a 15-45% sucrose gradient by using an automatic gradient mixer, slowly adding a PRRS virus liquid sample at the top of the sucrose gradient, and horizontally and centrifugally separating PRRS virus particles; connecting the sucrose gradient solution with a pumping system of a high performance liquid chromatograph, continuously detecting optical signals of the sucrose gradient solution through an ultraviolet and laser detector, and integrating a chromatographic peak to obtain a peak area; and calculating the content of the PRRS virus particles according to the regression equation of the ultraviolet absorption peak area and the protein concentration of the thyroglobulin standard substance. The method has better accuracy, repeatability and stability, and is suitable for measuring the antigen content of the PRRS virus particles.