95 results about "Chemiluminescent Assays" patented technology

The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemiluminescence is related to the amount of analyte in the sample.

The present invention relates to chemiluminescent assays which incorporate a second film or membrane which includes a solid chemical component for activation of a stable dioxetane. Decomposition of the stable dioxetane can be accomplished using a combination of heat and chemical treatment.

The invention discloses a full-automatic chemiluminiscence tester and belongs to the field of chemiluminiscence testing.The full-automatic chemiluminiscence tester comprises a mechanical arm module, a refrigeration module, a power module, a reagent sample disk, a sampling needle component, a stirring component, a reaction cup frame, an incubation device, a magnetic separation component, a washing station component and a measuring room.Full-automatic management is achieved for mixing, reacting, incubating, magnetic separating, detecting and other procedures of a sample to be tested, the detection efficiency is improved, and operation errors are reduced.

The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.

Disclosed herein is a polymeric sampling swab for obtaining samples of an analyte of interest from solid surfaces or from liquid substances. The polymeric material of which the swab is composed is characterized by a high resistance to chemical and mechanical degradation. The sampling swab of the present invention is further characterized by a high internal void volume and a high absorptive capacity for fluids. The swab of the present invention is particularly suited for obtaining samples for use in chemiluminescent assays for, among other analytes of interest, microbial contamination. Also disclosed is a polymeric disc for loading with reagent mixtures suitable for use in bioluminescent assay procedures. The reagent disc of the invention is characterized by high resistance to chemical and mechanical degradation. In addition, the disc has a high void volume and high absorptive capacity.

A hydrocarbon composition is provided containing:

at least 0.05 grams of hydrocarbons having boiling point in the range from an initial boiling point of the composition up to 204° C. (400° F.) per gram of the composition;

at least 0.1 gram of hydrocarbons having a boiling point in the range from 204° C. up to 260° C. (500° F.) per gram of the composition;

at least 0.25 gram of hydrocarbons having a boiling point in the range from 260° C. up to 343° C. per gram of the composition;

at least 0.3 gram of hydrocarbons having a boiling point in the range from 343° C. up to 510° C. per gram of the composition; and

at most 0.03 gram of hydrocarbons having a boiling point of greater than 538° C. per gram of the composition;

at least 0.0005 gram of nitrogen per gram of the composition, wherein at least 30 wt. % of the nitrogen in the hydrocarbon composition is contained in nitrogen-containing hydrocarbon compounds having a carbon number of 17 or less as determined by GC-GC nitrogen chemiluminscence.

A method is provided for determining in a medium the presence or amount of an analyte which is capable of binding to a ligand partner to form a ligand complex, which method comprises: (a) contacting the medium with either: (i) a ligand partner conjugated with an enzyme being capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, or (ii) a ligand partner and either a competing analyte or an analog of said analyte, a competing analyte or analyte analog being capable of forming a ligand complex with the ligand partner and the competing analyte or analyte analog being conjugated with an enzyme capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, (b) optionally separating complexed and uncomplexed enzyme conjugates, (c) causing or allowing the reaction or cascade of reactions to occur to produce hydrogen peroxide by contacting the complexed or uncomplexed enzyme conjugate with a corresponding enzyme substrate; (d) contacting hydrogen peroxide with a substance capable of exhibiting chemiluminescence in the presence of hydrogen peroxide selected from the group consisting of acridinium compounds and analogs thereof having a conjugate base with a pKa less then 9 to generate a chemiluminescent reaction, and (e) detecting the occurrence of said chemiluminescent reaction to determine the presence or amount of the analyte. Kits for use in connection with the method are also disclosed.

The invention belongs to the field of analytical chemistry and particularly relates to a method for detecting glycosylated hemoglobin by chemiluminescence. The method adopts the principle that chloroauric acid is reduced by luminol to obtain luminol colloid gold nanoparticles LumAuNPs, and LumAuNPs are modified with aptamer complementary sequences DNA to obtain chemiluminescent probes; aptamers DNA are fixed by taking magnetic beads as carriers to obtain DNA modified magnetic beads; the chemiluminescent probes and the DNA modified magnetic beads are incubated to obtain chemiluminescence sensors; when a target object (glycosylated hemoglobin) exists, the aptamers DNA and glycosylated hemoglobin act with each other, and the chemiluminescent probes drop off the surfaces of the magnetic beads; and after magnetic separation, hydroxylamine-O-sulfonic acid is added into a separating medium, and LumAuNPs-hydroxylamine-O-sulfonic acid is taken as a chemiluminescent system, chemiluminescence assay is performed, and the target glycosylated hemoglobin is detected according to the generated chemiluminiscence. The method has the advantages of being simple and being high in sensitivity.

The invention discloses a method for determining cholesterol by using flow injection chemiluminescence with nano-copper oxide as a catalyst. The method comprises the following steps: mixing cholesterol, cholesterol oxidase and phosphatic buffer solution; warm bathing the mixture solution to obtain a reaction solution; after diluting the reaction solution, inputting the diluted reaction solution into a mixer through a peristaltic pump to mix with a nano-copper oxide colloidal solution; then mixing with a luminol solution; entering into a flow cell for reaction; and detecting the generated chemiluminescence intensity by a photomultiplier to determine the cholesterol. By using cholesterol oxidase for catalyzing the oxidation of cholesterol to generate hydrogen peroxide, combining the flow injection technique, the chemiluminescence signal in the determination of cholesterol is enhanced about 600 times. By using cholesterol-cholesterol oxidase-luminol-nano-copper oxide flow injection chemiluminescence to determine the cholesterol content, the linear range is 0.625-12.5 [mu]mol/L. the method can be used for determination of food cholesterol content and determination of the free cholesterol and total cholesterol in serum.

The invention discloses a chemiluminescence measuring method. First Hall sensors are respectively arranged on a rotating platform and at a cup placing station, a liquid taking station, a liquid mixing station, a hatching station, a cleaning station, a reading station and a cup dropping station, and are used for sensing the positions where a reaction cup reach the stations; a magnet conducts circular motion along with a rotary table under the action of a motor; when the rotary table rotates to the corresponding position, the magnet and the corresponding Hall sensor generate induction, the rotary table stops, and the position is subjected to corresponding detection operation. The chemiluminescence measuring method is accurate in detection and easy to implement. A corresponding measuring system is rich in functions, parts are smooth in operation, can operate mutual cooperatively, are compact and reasonable in structural design, and cooperate together, complex measurement processes can be accomplished, and the automation degree is high.

The invention relates to a method for detecting the content of bleomycins (BLMs), belonging to the technical fields of electrochemical luminescence and sensors. According to the method, an electrochemical luminescence agent is utilized for marking DNA (deoxyribonucleic acid) to prepare a probe (Ru-DNA), and a carboxylated carbon nanoparticle modified electrode is utilized for constructing a carboxylated carbon nanoparticle modified electrode electrochemical luminescence biosensor through a competitive effect between an electrostatic repulsion force and a pi-pi accumulation action on the basis of non-covalent-bond self-assembly principles of the DNA and the carbon nanoparticle. Target objects, namely the bleomycins, act on the DNA probes to cut the DNA, the DNA probes adsorbing on the carbon nanoparticle modified electrode are reduced, and weak electrochemical luminescence signals are generated, thereby realizing the measurement of the bleomycins. The method has the advantages of simplicity and rapidness.

The invention discloses an incubation and magnetic separation device applied to chemiluminescence determination and belongs to the field of chemical luminescence determination. The incubation and magnetic separation device applied to the chemiluminescence determination comprises an incubation device and a magnetic separation device, wherein the magnetic separation device is arranged at one side of the incubation device, so that a magnet on a magnet frame corresponds to a bottom side face of a reaction cup on a reaction cup hole. The incubation magnetic separation device integrates an incubation function and a magnetic separation function; a temperature range and magnetic selection time are accurate and adjustable; the device is stable in properties and simple to operate. The incubation device adopts contact type direct heating and has a good incubation effect; the magnetic separation device has the advantages of good buffering effect and reduction of loss of magnetic beads and the like.

The invention discloses a chemiluminescence measuring system. The chemiluminiscence measuring system comprises a pedestal, a rotating platform, a liquid sampling device, a washing device, a liquid injecting/uniform mixing device, a incubation device, a reading device, and a cup discarding device; the rotating platform is arranged at the center of the pedestal; the rest devices are arranged on the pedestal around the rotating platform; a cup placing position, a liquid sampling position, a liquid uniform mixing position, an incubation position, a washing position, a reading position, and a cup discarding position of the rotating platform are provided with a first Hall sensor respectively for sensing the position of a reaction cup at each work station; a magnet is driven by a motor to perform circling motion together with a rotating disc; when the rotating disc rotates to a corresponding position, induction of the magnet with a corresponding Hall sensor is realized, the rotating disc is stopped, and corresponding measuring operation is carried out at the position. The functions of the chemiluminescence measuring system are abundant; smooth operation and cooperative working of the parts are realized; the structure is designed to be compact and reasonable; complex measuring process can be completed; and automation degree is high.

Based on recent investigations showing iatrogenic, profound neutropenia (and the fever spikes that often accompany it, which must be medicated immediately to avoid the risk of life-threatening infection) can most accurately be monitored by obtaining daily mucosal neutrophil counts from the patient's oral mucosa rather than obtaining daily counts of the patient's blood neutrophils as in the past, a mouth wash method has been developed for collecting muscosal neutrophils. The mouth wash samples so collected are delivered directly, or in aqueous dilution to a sample pad supported on a strip which sample pad has deposited thereon reagents enabling a colorimetric, fluorescent or chemiluminescent assay of the quantity of an enzyme characteristic of human neutrophils that is present in the sample. This measured quantity can be correlated to mucosal neutrophil count. The method shows outstanding sensitivity, precision and accuracy relative to microscopic methods of counting mucosal neutrophils.

A method for assay of an analyte by use of a material labeled with a chemiluminescent substance, which comprises adding a quencher and/or decreasing the specific activity of a chemiluminescent substance labeled probe, thereby decreasing the quantity of chemiluminescence.

The invention belongs to the field of blood testing, and relates to a homogeneous phase multi-index fluorescence/chemiluminescence measuring method and application thereof. The method comprises the steps of: fixing a plurality of specificity recognition monoclonal antibodies on a polystyrene microsphere, and reacting with a plurality of labeled antibody cores modified by haptens after combining with carcinoembryonic antigens, cell keratin fragments 19, neuron specificity enolase and other multiple markers; reacting with horse radish peroxidase modified by corresponding hapten antibodies, alkaline phosphatase and quantum dots, and measuring a plurality of markers on the same instrument by combining with detections of chemiluminescence, fluorescence and the like. The method has the advantages of small blood serum consumption, rapid detection time, low diagnosis cost and simple operation, is suitable for detecting a plurality of popularized indexes simultaneously, and can provide auxiliary judgment basis for the early diagnosis of critical illnesses of tumor and the like.