149 results about "Peroxiredoxin activity" patented technology

The present invention provides a novel chemiluminescent reagent producing chemiluminescence in the presence of hydrogen peroxide, extent of which depends on peroxidase concentration, chemiluminescent analysis method using the same, in particular useful for detection and quantitative analysis of various types of materials by measuring peroxidase enzyme activity or enzyme immunoassay with peroxidase enzyme as the marker.More particularly, the present invention provides a chemiluminescent reagent containing, as the major ingredients, a charge-transferring complex of N,N'-disubstituted-9,9'-bisacridinium salt and N,N-disubstituted carboxylic amide compound; chemiluminescent reagent containing further containing a specific aminoalcohol compound, in addition to the above; and method for measuring peroxidase activity at a high sensitivity in the presence of a peroxide, using the above chemiluminescent reagent.Moreover, the novel chemiluminescent reagent of the present invention can enhance sensitivity of the enzyme immunoassay with peroxidase enzyme as the marker by its chemiluminescent reaction.

A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final “read zone” wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays.Specific assays for glucose-6-phosphate dehydrogenase (“G-6PD”), total serum cholesterol, β-lactamase activity and peroxidase activity are disclosed.

The present invention relates to methods for increasing hydrolysis of a cellulosic material, comprising: hydrolyzing the cellulosic material with an enzyme composition in the presence of a polypeptide having peroxidase activity.

A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final "read zone" wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays. Specific assays for glucose-6-phosphate dehydrogenase ("G-6PD"), total serum cholesterol, beta-lactamase activity and peroxidase activity are disclosed.

The invention discloses a preparation method for a mimic enzyme based on a self-assembly structure. The preparation method comprises the following steps: (1) dissolving chemical substances capable of carrying out a self-assembly behaviour into water; (2) adding an active metal salt solution and a reducer in the solution in the step (1), and stirring to obtain the mimic enzyme based on the self-assembly structure. In the preparation method provided by the invention, two previously prepared chemical substance aqueous solutions capable of carrying out the self-assembly behaviour are mixed, two types of molecules are assembled together through an action force among weak molecules, and metal salt ions are adsorbed on the surfaces of the molecules and generate metal nanoparticles in situ under the action of the reducer; and the obtained metal nanoparticles based on the self-assembly structure have a great peroxidase activity under the conditions of wide pH and temperature ranges, high ion strength and the like. The synthesis conditions of the material are moderate, simple and convenient, the price of raw materials is low, and business development is facilitated. The metal nanoparticle material based on the self-assembly structure, which is disclosed by the invention, can also be applied to many fields of electro-catalysis, water treatment, biomedicine and the like, and has a wide application prospect.

The invention discloses a colorimetric analysis method for detecting kanamycin based on aptamer-modified magnetic beads and gold nanoparticles to simulate enzyme activity, and belongs to the technical field of analytical chemistry. In the present invention, the gold nanoparticles synthesized by tyrosine reduction of chloroauric acid simulate the peroxidase-like activity inherent in the enzyme AuNPs, and the gold nanoparticles modified by kanamycin-specific aptamers modify their complementary single-stranded cDNA The capture of nanoparticles, and the displacement of gold nanoparticles by the combination of kanamycin and aptamer, the peroxidation-like peroxidation of gold nanoparticles AuNPs contained in the supernatant after magnetic separation is related to the concentration of kanamycin The enzyme activity catalyzes the color reaction between the substrate tetramethylbenzidine (TMB) and H2O2, realizes the visual detection of kanamycin, and uses the linear relationship between the absorption value at 450 nm and the concentration of kanamycin to realize the detection of kanamycin. Colorimetric quantitative analysis of kanamycin. This method has the advantages of high sensitivity and good specificity, and is suitable for the quantitative analysis of kanamycin residues in food samples such as honey.

The invention discloses a strawberry seedling substrate, and a production method and application thereof. The substrate comprises the following components: earthworm cast, peat, perlite and vermiculite, wherein the volume ratio of earthworm cast, peat, perlite to vermiculite is (1-4):(0-4):1:1; the earthworm cast is a product formed by earthworms taking cow dung. According to the strawberry seedling substrate, and the production method and application thereof, peat is replaced with the earthworm cast obtained by raising the earthworms fed with cow dung, so that resourceful utilization channels of the earthworm cast are expanded, environmental pollution is reduced, the ecological environment is protected, and the recycling and low-carbon circulation of resources are realized; adequate nutrients can be provided for strawberries in a seedling period, chlorophyll synthesis can be promoted, and the activity of peroxidase in strawberry leaves can be improved; the rooting of maternal seedlings and the growth of creeping stems are promoted, the strawberry seedling quality is improved, the rooting rate is increased, high survival rate and seedling uniformity in the seedling period are ensured, the number of strong seedlings is remarkably increased, seedling lifting labor is saved, and convenience in operation and transportation is ensured.

This invention provides antioxidant cyclic salen-metal compounds, compositions of such antioxidant cyclic salen-metal compounds having superoxide activity, catalase activity and / or peroxidase activity and methods of using such antioxidant cyclic salen-metal compositions to treat or prevent a disease associated with cell or tissue damage produced by free radicals, such as superoxide.

The invention provides a method for inhibiting peroxidase activity by using fluorescent graphene quantum dots. The method comprises the following steps: (1) adding a carbon source into a certain amount of water to prepare a mixed solution, heating the solution until the moisture is completely evaporated, transferring the solution into a high pressure reactor, heating and reacting for a period of time, dissolving the product in water, regulating the pH value of the system to be neutral by using alkali liquor, purifying the product, and drying, thereby obtaining solid graphene quantum dots; and (2) dispersing the quantum dots obtained in the step (1) in secondary distilled water, taking a proper amount of quantum dot solution and enzyme solution, adding the solution into a buffer solution, adding a color developing agent and H2O2 after a period of time, uniformly shaking the mixed solution, rapidly adding the solution into a cuvette, measuring the light absorption value of the maximum absorption wavelength of the product on an ultraviolet-visible spectrophotometer, and performing blank sample control, thereby determining the relative enzyme activity. The graphene quantum dots are prepared by adopting low-price and readily available raw materials, and the method is green and environment-friendly. The structure of the enzyme can be changed by virtue of the interaction between the graphene quantum dots and zymoprotein, and regulation of the enzyme activity is realized.

The invention relates to a miRNA (Micro Ribonucleic Acid) detection probe and a method for visually detecting miRNA. The miRNA detection probe comprises three parts: a G-quadruplex forming sequence close to a 3' end, a miRNA complementary sequence of a target to be measured in the middle, and an interference sequence at a 5' end. The probe has a hairpin structure under general conditions. After the target is added, the quantity of base pairs and stability of a DNA-RNA (Deoxyribonucleic Acid-Ribonucleic Acid) heterozygote are higher than that of a DNA double chain formed by the interference interface, and a hairpin structure is opened. The opened hairpin structure can release the G-quadruplex forming sequence under the action of a double-chain specific incision enzyme, G-quadruplex is formed under the action of sodium and potassium ions, and a catalyst with peroxidase activity is produced by assembling with Hemin. The method for detecting miRNA disclosed by the invention only requires one probe, a generated signal is visual, and expensive instruments are not required.

A preparation with the activities of laccase, xylanase and peroxidase for paper-making, environment production, food and feed industries is prepared through cloning new genes of laccase, xylanase and peroxidase, configuring their expression carrier, introducing it to yeast or mycelial fungus for expression, checking the activity of recombinant enzymes, and mixing said recombinent enzymes together.

The present invention provides peptide-based peroxidase inhibitors having the formula AA1-AA2-AA3, wherein AA1 is a positively charged, negatively charged or neutral amino acid, AA2 is a redox active amino acid, and AA3 is an amino acid possessing a reducing potential such that AA3 is capable of undergoing a redox reaction with a radical of amino acid AA2 or a retro or retro-inverso analog thereof. The result of such a combination is a highly effective inhibitor of peroxidase activity that has potent anti-inflammatory properties in widely diverse models of vascular disease and injury. Exemplary tripeptides effectively inhibit peroxidase mediated LDL oxidation, increase vasodilation in SCD mice, inhibit eosinophil infiltration and collagen deposition in asthma mice, inhibit acute lung injury, and decrease ischemic injury of the heart.

The invention belongs to the field of high polymer materials and biological medicines, and relates to a ferroferric oxide loaded nickel carbide layer nanotube and a preparation method and applicationthereof. The ferroferric oxide loaded nickel carbide layer nanotube (Fe3O4@C-NT / Ni) is in the shape of a hollow tube, has excellent electromagnetism, catalytic activity, biological compatibility and water dispersibility, has activity similar to that of peroxidase, and is used for detecting and analyzing hydrogen peroxide and bio-active substances. The preparation method of Fe3O4@C-NT / Ni is simpleand efficient, high in recycling rate, safe and environmentally friendly. The catalytic activity of Fe3O4@C-NT / Ni is similar to that of peroxidase, Fe3O4@C-NT / Ni is used for redox reaction of hydrogenperoxide as an electron acceptor, and qualitative and quantitative measurement of the hydrogen peroxide is realized; and Fe3O4@C-NT / Ni is also used for qualitative and quantitative measurement of bio-active substances which take the hydrogen peroxide as a constant-ratio product. Compared with the prior art, the method is simple and speedy, economical and environmentally friendly, wide in detection line, high in sensitivity, good in selectivity and visual.

The orally taken antioxidant and antilipemic medicines include solid orally taken preparation prepared with forsythin and solid excipient in the weight ratio of 0.01-4 through mixing and conventionaltechnological process, and liquid orally taken preparation prepared with forsythin and liquid excipient in the weight ratio of 0.003-0.01 through mixing and conventional technological process. Test shows that forsythin as the active component of the present invention has excellent effect of clearing away hydroxy radical and can inhibit the oxidizing damage in tissue and cell organ level. The medicine of the present invention has also functions of delaying senility and reducing blood fat, etc.

The invention provides an electrochemical detection method for detecting kanamycin residues based on a nucleic acid aptamer and a nano analogue enzyme and belongs to the technical field of analytical chemistry. Gold nano-particles are synthesized by reducing chloroauric acid through tyrosine, and hydrogen peroxide and reduced-state thionine are catalyzed to react to generate oxidized-state thionine; and the thionine can be detected through a difference pulse voltammetry. The gold nano-particles are modified through utilizing a kanamycin specific aptamer; and the aptamer is adsorbed on the surfaces of the gold nano-particles so that the peroxidase activity is inhibited. When a target object kanamycin exists, the aptamer can be competitively replaced from the surfaces of the gold nano-particles to form a compound, so that the peroxidase activity is recovered. The detection of the kanamycin can be realized through detecting a relation between a reduction peak current value of the oxidized-state thionine and an antibiotic concentration by utilizing the difference pulse voltammetry. The method provided by the invention has good repeatability, good stability and high sensitivity, and can be used for effectively detecting kanamycin residues in food samples.

The present invention provides a method for selective production of theaflavin in large amounts at high yield, and in an easy and inexpensive manner. Specifically, it relates to a method for selective production of theaflavin whereby a processed plant extract containing epicatechin, epigallocatechin, epicatechin-3-O-gallate and epigallocatechin-3-O-gallate is combined with a plant cell culture having peroxidase activity for selective production of theaflavin.

A trivalent iron-porphyrin and its derivative-short peptide compound and its synthesizing process are disclosed. Said compound is composed of heme or subheme and short peptide, and is prepared from heme or subheme and amino acid derivative as raw materials and dimethyl formamide as solvent through swelling, removing protecting, coupling, cyclic removing protecting and coupling, coupling heme or subheme and severing. Its advantages are high antioxidizing activity and the activity of peroxidase, high purity and low toxin.

The invention provides a method for rapid identification of a heat-resistance seedling state of cotton, which comprises the following steps of: treating cotton seedlings at trefoil stage to quatrefoil stage for 5-12 hours at the temperature of 40-45 DEG C; setting comparison under normal growth condition; respectively determining ascorbic acid peroxidase activity, relative electric conductivity and seedling withering rate of treated cotton seedlings and normal growing cotton seedlings; calculating relative values of the ascorbic acid peroxidase activity, the relative electric conductivity and the seedling withering rate relative to those of the normal growing cotton seedlings; and determining heat resistance. The method can be used for effectively identifying the heat resistance of different cotton germplasm resources rapidly, and can provide appropriate parent materials for high-temperature resistant seed breeding.

A composite litter material for detecting a disease, such as urinary tract disease, when contacted by feline urine includes an absorptive polymer material forming a solid matrix, exfoliated clay embedded and dispersed within the solid matrix, a chromogenic indicator provided within the solid matrix, and an oxidizing agent distributed and stabilized within the solid matrix to be responsive to peroxidase activity in the urine to activate the chromogenic indicator. A method for detecting or diagnosing a feline disease includes contacting urine with the material and detecting a color change. A method of manufacturing the composite material includes preparing a mixture comprising the absorptive polymer and water and distributing the exfoliated clay, chromogenic indicator and oxidizing agent within the mixture, preferably by extrusion and addition of the components at particular points in processing the polymer mixture.

The invention discloses a molecular marker used for identifying POD (peroxidase) activity of a wheat grain and an application of the molecular marker. The molecular marker comprises primer pairs shown in (1) and (2) as follows: (1) represents a primer pair 1 comprising single-chain DNA shown in SEQ ID No.1 and single-chain DNA shown in SEQ ID No.2; (2) represents a primer pair 2 comprising single-chain DNA shown in a SEQ ID No.3 and single-chain DNA shown in SEQ ID NO.4. As is proven by an experiment, the molecular marker can be used for quickly identifying TaPod-D1a and TaPod-D1b allelic variations and correlation between TaPod-D1a and TaPod-D1b allelic variations and POD activity of wheat, a wheat variety with relatively high POD activity is screened out, the cultivation speed of the new high-quality wheat variety is increased, and the molecular marker has great theoretical significance and economical value in assisting in selection of the wheat variety with high POD activity.

The invention discloses palladium nano-particles with peroxidase activity and a preparation method thereof. The preparation method includes the following steps that (1) annealing of deoxynucleotide is conducted, specifically, a deoxynucleotide aqueous solution and a phosphate buffered solution are added into a 1.5 mL centrifuge tube and mixed evenly, and the temperature is increased to 90-100 DEG C, maintained for 5-10 min, and decreased to the room temperature; and (2) the nano-particles are prepared, specifically, a sodium tetrachloropalladate aqueous solution is added to the annealed deoxynucleotide aqueous solution obtained in the step (1), the mixture is mixed evenly and stands, a reducing agent aqueous solution is added, the mixture is mixed evenly and stands, and liquid of the palladium nano-particles with peroxidase activity is obtained. The method disclosed by the invention is simple, the period is short, conditions are easy to control and mild, and large-scale industrial production and preparation are facilitated. The mean grain size of the nano-particles with peroxidase activity is 1.0-4.8 nm, and the nano-particles with peroxidase activity is high in catalytic activity.

The invention discloses a method for controlling a pineapple's blackheart disease by jasmonic acid methyl ester. The method comprises the steps that 25 days to 40 days before pineapples are picked, a jasmonic acid methyl ester water solution of 1-100 mu mol / L is sprayed on the surfaces of pineapple fruits; and the pineapples are picked, then aired and stored in a refrigeration storage at the temperature of 8-10 DEG C. According to the method, the 1-100 mu mol / L jasmonic acid methyl ester water solution is sprayed on the surfaces of the pineapple fruits during a picking time, so that the polyphenol oxidase activity and the peroxidase activity of the pineapples can be reduced effectively, and the pineapples are prevented from suffering from the blackheart disease. The method is simple in operation, low in cost and high in applying efficiency, and can be popularized easily; at the same time, the storage period of the pineapples is prolonged, the economic loss of a dealer can be reduced effectively, the problem of a farmer that the pineapples are difficult to sell is solved, and the enthusiasm of the farmer for planting pineapples is protected; and the pineapples also can be sold to countries all over the world by ship, more foreign currencies are earned for the country of the farmer, and good economic and social benefits are achieved.

The invention discloses a preparation method of a carbon quantum dot, nano gold, and a composite film and a combined electrode. The preparation method of the carbon quantum dot comprises following steps: taking sodium hydroxide as electrolyte and ethylene glycol as the precursor of the carbon quantum dot, preparing a mixed water solution of sodium hydroxide and ethylene glycol; electrifying the mixed water solution to carry out electrolysis; after electrolysis, adjusting the pH of the solution to neutral; after pH adjustment, and subjecting the solution to dialysis to obtain a carbon quantum dot water solution. Ethylene glycol is taken as the precursor, carbon quantum dot is synthesized by a one-step electrolysis method, the carbon quantum dot has the advantages of good water solubility and high stability; the surface of the carbon quantum dot comprises a large amount of hydroxyl groups and carboxyl groups; the excellent reducing performance and stability of the carbon quantum dot areused to reduce chloroauric acid so as to prepare nano gold; the prepared nano gold is highly dispersed, the repeatability is good, and moreover, the nano gold is stable during the storage process andreaction process and has enzymatic activity of peroxides.

The invention relates to the field of nanometer materials, catalysis and analysis, in particular to iridium nano-enzyme having activity of tandem enzyme and application thereof. The iridium nano-enzyme has the activity of imitated peroxidase and the activity of imitated glucose oxidase, and the activity of imitated enzymes is similar to an optimum active pH. The iridium nano-enzyme is utilized toachieve one-pot non-enzyme developing glucose detection and is hopeful to be widely used in the fields such as analytical chemistry, biological medicine, food engineering and catalysis.

The invention discloses a method for controlling a blackheart disease of normal-temperature stored pineapples by an abscisic acid. The method comprises the steps that an abscisic acid solution of 5-500mg / L is sprayed on the surfaces of the pineapples; and the sprayed pineapplesare is packed with a polyethylene plastic film bag of 0.02-0.04 millimetres and moisturized for 6-9 hours, then the polyethylene plastic film bag is removed, and the pineapples are aired and stored in a storehouse at the temperature of 20-25 DEG C. According to the method, the abscisic acid solution of 5-500mg / L is sprayed on the surfaces of pineapple fruits, so that the polyphenol oxidase activity and the peroxidase activity of the pineapples can be reduced effectively, and the pineapples can be prevented from suffering from the blackheart disease. The method is simple in operation, low in cost and high in applying efficiency, and can be popularized easily; at the same time, the storage period of the pineapples is prolonged, the problem of a farmer that the pineapples are difficult to sell can be solved effectively, and the enthusiasm of the farmer for planting pineapples is protected; and good economic and social benefits are achieved. The pineapples are kept fresh without a refrigerating storage and being transported at a low temperature, so that the energy is saved, the environmental protection is facilitated, and the method is a low-carbon preservation technology.

The invention discloses application of gamma-aminobutyric acid in improving salt stress resistant capacity of corn, relates to application of gamma-aminobutyric acid in growth and development of the corn, and aims at providing new application of gamma-aminobutyric acid. Gamma-aminobutyric acid has a regulating effect on oxidative damage and enzymatic activity of corn leaves under salt stress, the content of malonaldehyde in corn seedling leaves under the salt stress is decreased, the content of superoxide anions in the leaves is decreased, the content of superoxide dismutase in the leaves is decreased, improvement of peroxidase activity is promoted, the activity of an antioxidase system under NaCl stress is improved, the regulating effect on corn seedling nitrogen metabolism under the salt stress is achieved, the content of soluble protein of the corn leaves and root systems under the salt stress is increased, and pyruvic acid content, glutamine synthetase activity, glutamate synthase activity, glutamate dehydrogenase activity and glutamate decarboxylase activity in the corn leaves and the system roots under the salt stress are decreased. The application of gamma-aminobutyric acid in improving the salt stress resistant capacity of the corn is used in the field of corn cultivation.

The present invention discloses an electrophoresis titration method for determining the activity of peroxidase. The electrophoresis titration method comprises: filling the channel part of a chip with a gel, injecting the mixed solution a of a hydrochloric acid solution and a background electrolyte solution into an anode pool, and injecting the mixed solution b of a chemiluminescent base solution and a background electrolyte solution into a cathode pool; injecting a peroxidase solution into the cathode pool to catalyze the chemiluminescent base solution to react so as to produce excitation state luminol anions L*; after the enzyme catalytic reaction is stabilized, applying an electric field to make the excitation state luminol anions L* generated in the cathode pool and the hydrogen ions H<+> in the anode pool form MRB, wherein the generated MRB moves to the anode; and according to the functional relationship between the MRB movement rate and the activity of the peroxidase, determining the activity index of the peroxidase. With the method of the present invention, various enzyme indexes of the peroxidase can be effectively and rapidly determined without complex optical detection equipment.

The present invention is related to accurate detection methods for the measurement only of myelopexidase (MPO) levels or neutrophils, preferably equine neutrophils, in complex biological samples. The present invention is further related to ELISA and SIEFED assays for such detection. SIEFED detection sensitivity of active peroxidase activity was found to be enhanced by the addition of nitrite. Such MPO measurement finds its use in many applications such as the prediction, diagnosis and / or monitoring of pathologies correlated with neutrophil activation and / or destruction; the evaluation of drugs and / or immunomodulators; the assessment of immune responses, either natural and / or after treatment with immunomodulators and / or drugs; and the study of cells and their ability to fight microorganisms and / or to destroy them.

The invention belongs to the technical field of analytical chemistry, and in particular relates to a nitrogen-doped ferroferric oxide peroxide mimic enzyme and a glucose detection method thereof. Nitrogen substance urea modification is performed on Fe3O4 nanometer mimic enzyme to synthesize N-Fe3O4 nanometer mimic enzyme and research the catalytic activity of the Fe3O4 nanometer mimic enzyme; compared with other modifiers, due to the introduction of nitrogen, the peroxidase activity and catalytic efficiency of the Fe3O4 nanometer mimic enzyme can be significantly improved; a stable hydrogen bond is formed between -NH2 of the Fe3O4 nanometer mimic enzyme and H2O2, and thus the appetency with the H2O2 is improved; by using the characteristic of catalyzing H2O2, in combination with the glucose oxidase that catalytically oxidizes glucose to generate H2O2, the method for quickly detecting the glucose content is built; compared with the prior art, the method has lower detection limit, widerlinear range and better specificity, and the better method is provided for measuring the glucose content in the actual sample.