Mimic enzyme based on self-assembly structure as well as preparation method and application thereof

Abstract

The invention discloses a preparation method for a mimic enzyme based on a self-assembly structure. The preparation method comprises the following steps: (1) dissolving chemical substances capable of carrying out a self-assembly behaviour into water; (2) adding an active metal salt solution and a reducer in the solution in the step (1), and stirring to obtain the mimic enzyme based on the self-assembly structure. In the preparation method provided by the invention, two previously prepared chemical substance aqueous solutions capable of carrying out the self-assembly behaviour are mixed, two types of molecules are assembled together through an action force among weak molecules, and metal salt ions are adsorbed on the surfaces of the molecules and generate metal nanoparticles in situ under the action of the reducer; and the obtained metal nanoparticles based on the self-assembly structure have a great peroxidase activity under the conditions of wide pH and temperature ranges, high ion strength and the like. The synthesis conditions of the material are moderate, simple and convenient, the price of raw materials is low, and business development is facilitated. The metal nanoparticle material based on the self-assembly structure, which is disclosed by the invention, can also be applied to many fields of electro-catalysis, water treatment, biomedicine and the like, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of macromolecular compounds, and specifically discloses a self-assembled structure-based imitating enzyme, a preparation method and application thereof. Background technique [0002] Compared with proteases, although nanomaterial mimic enzymes have good acid, alkali and high temperature stability, low cost, simple synthesis, can be prepared in large quantities, and are easy to be modified and labeled by various functional molecules, there is still a big gap. The challenges we face are still severe. Common nanomaterial mimic enzymes Fe3O4, CeO2, nano-gold derivatives, graphene derivatives, etc. will be used to combine specific oxidases (such as glucose oxidase, etc.) and color reagents (such as 3,3',5 , 5'-tetramethylbenzidine TMB, etc.) for the detection of specific molecules. Although a certain detection effect has been achieved, it is difficult to be commercially developed and applied in people's daily life. The m...

AI Technical Summary

Problems solved by technology

Although a certain detection effect has been achieved, it is difficult to be commercially developed and applied in people's daily life. The main reasons are: a) Easy-to-prepare nanomaterials mimic the high catalytic activity of enzymes in acidic environments and neutral conditions Almost no catalytic effect, while natural enzymes (such as glucose oxidase, etc.) have a good catalytic effect under neutral conditions, proteases may be inactivated under acidic conditions, it is difficult to combine proteases in the development of kits and test papers

Although it has been reported that a nanomaterial imitating enzyme that can exert high catalytic activity under neutral conditions has been prepared, the harsh preparation conditions are difficult to be commercially applied; b) the catalytic activity is low, although some nanomaterial imitating enzymes can be used However, its catalytic activity is still not comparable to that of horseradish peroxidase, so it is difficult to be used in the development of ELISA kits

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