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DNA functionalized copper oxide nano-enzyme as well as preparation method and application thereof
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A nano-enzyme and copper oxide technology, applied in the field of nano-carriers and nano-biological materials, to achieve tumor targeting, increase ROS levels, and simple operation
Active Publication Date: 2021-03-02
湖南修悟生物科技有限公司
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Problems solved by technology
However, based on the law of DNA adsorption, the design of DNA-functionalized nanozymes has not been reported in the targeted therapy of tumors.
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Embodiment 1
[0046]A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a di-block DNA, one end is (TC)12DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-A15(SEQID NO. 2: AAAAAAAAAAAAAAA), di-DNA is adsorbed on CuO nanozyme through a DNA sequence composed of TC bases.
[0047]A preparation method of the polarization-controlled DNA functionalized CuO nanozyme of the present invention includes the following steps:
[0048](1) Disperse 1 μM di-DNA in 10 mM HEPES buffer (pH 7.6, 150 mM NaCl) to obtain a dispersion;
[0049](2) Adsorb 100μg / mL CuO nanozyme and dispersion at 4℃ for 2h to obtain polarization-controlled DNA functionalized CuO nanozyme;
[0050](3) Add 1% FBS that has been inactivated by high temperature, mix well with ultrasound, and continue to incubate for 1 hour.
[0051](4) Then centrifuge at 4°C and 16000 rpm for 30 min, remove the supernatant, wash the precipitate, and disperse in HEPES buffer (10 mM, pH 7.6, 150 mM NaCl) for us...
Embodiment 2
[0053]A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a di-block DNA, one end is (TC)12DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-T15(SEQID NO.3: TTTTTTTTTTTTTTT), di-DNA is adsorbed on CuO nanozyme through a DNA sequence composed of TC bases.
[0054]The preparation method is the same as in Example 1.
Embodiment 3
[0056]A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a di-block DNA, one end is (TC)12DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-C15(SEQID NO. 4: CCCCCCCCCCCCCCC), di-DNA is adsorbed on CuO nanozyme through a DNA sequence composed of TC bases.
[0057]The preparation method is the same as in Example 1.
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Abstract
The invention provides DNA functionalized copperoxide nano-enzyme as well as a preparation method and application thereof. The DNA functionalized copperoxide nano-enzyme comprises di-DNA and CuO nano-enzyme, one end of the di-DNA is a DNA sequence composed of TC bases, the other end is a DNA sequence composed of nucleoside, and the di-DNA is adsorbed on the CuO nano-enzyme through the DNA sequence composed of the TC bases. The DNA functionalized copperoxide nano-enzyme is designed on the basis of an adsorption mechanism of DNA on the surface of the copper oxide nano-enzyme, and the DNA canbe polarized and adsorbed on the surface of the nano-enzyme and endows the nano-enzyme with a tumor targeting function. The preparation method comprises the following steps: dispersing di-DNA into a buffer solution to obtain a dispersion solution; and mixing the dispersion solution with CuO nano-enzyme, and performing adsorbing. The preparation method disclosed by the invention is simple to operate, low in cost and mild in reaction conditions, can be used for tumor targeted modification of the nano-enzyme, and has a wide application prospect in the fields of disease treatment and the like.
Description
Technical field[0001]The invention relates to the fields of materials, nano-carriers and nano-biotechnology, in particular to a DNA-functionalized copper oxide nano-enzyme, and a preparation method and application thereof.Background technique[0002]Tumor microenvironment (TME) is an abnormal microenvironment with low oxygen, slight acid and high levels of H2O2specialty. Using the significant differences between TME and normal tissues, various anti-tumor treatment strategies can be customized. For example, anaerobic Salmonella can multiply in TME in the hypoxic zone. Using this feature, a tumor-targeted drug deliverysystem with Salmonella can be constructed; nanoenzymes assembled from acid-sensitive polymers can respond to acidic TME to achieve tumors. Targeting; or use the high level of hydrogenperoxide in TME to generate singlet oxygen and enhance the combined anti-tumor efficiency.[0003]Nanoenzyme is a kind of artificial mimic enzyme with high catalytic activity. Compared with na...
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