DNA-functionalized copper oxide nanozyme, preparation method and application thereof
A nano-enzyme and copper oxide technology, applied in the fields of materials, nano-carriers and nano-biology, achieves the effects of mild reaction conditions, simple operation, and increased ROS levels
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Embodiment 1
[0046]A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-A 15 (SEQID NO.2: AAAAAAAAAAAAAAA), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0047] A method for preparing the polarization-controlled DNA functionalized CuO nanozyme of the present invention, comprising the following steps:
[0048] (1) Disperse 1 μM di-DNA in 10 mM HEPES buffer (pH7.6, 150 mM NaCl) to obtain a dispersion;
[0049] (2) Adsorb 100 μg / mL CuO nanozyme and dispersion liquid at 4°C for 2 h to obtain polarization-controlled DNA functionalized CuO nanozyme;
[0050] (3) Add 1% FBS inactivated by high temperature, mix well by ultrasonic, and continue to incubate for 1 h.
[0051] (4) Centrifuge at 4° C., 16000 rpm for 30 min, remove the supernatant, wash the precipitate, disperse in HEPES buffer (10 mM, pH 7.6...
Embodiment 2
[0053] A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-T 15 (SEQID NO.3: TTTTTTTTTTTTTTT), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0054] Its preparation method is consistent with embodiment 1.
Embodiment 3
[0056] A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-C 15 (SEQID NO.4: CCCCCCCCCCCCCCC), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0057] Its preparation method is consistent with embodiment 1.
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