59results about How to "Increased peroxidase activity" patented technology

The invention discloses a folic acid-porous platinum-graphene oxide composite nano material as well as an application thereof in detecting tumor cells. The folic acid-porous platinum-graphene oxide composite nano material is prepared by the following steps: by taking graphene oxide as a substrate, carrying out amidation to crosslinked folic acid molecules; and carrying out in-situ synthesizing on platinum nano particles with porous structures. The folic acid-porous platinum-graphene oxide composite nano material can specifically identify a folate receptor on the surface of the cell, and meanwhile has a characteristic of simulating peroxidase and can catalyze hydrogen peroxide oxidized 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to develop color. The number of the tumor cells can be quickly and agilely detected by means of chromogenic reaction, and 30 MCF-7 (Michigan Cancer Foundation-7) cells can be detected to the minimum extent. The material further can catalyze diaminobenzidine to generate a reddish brown precipitate on the surface of the cell so as to realize targeted positioning detection.

The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.

The invention discloses a platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase. Sulfonated graphene is taken as the substrate, platinum nano particles are synthesized by a sodium borohydride reduction method, and sulfonated graphene and platinum nano particles are used to prepare the platinum-sulfonated graphene composite nano material. The platinum-sulfonated graphene composite nano material has an excellent activity of peroxidase and can catalyze the oxidation reactions between 3,3',5,5'-tetramethylbenzidine hydrochloride to trigger color development. The composite nano material has good affinity on the substrate namely 3,3',5,5'-tetramethylbenzidine hydrochloride. The composite nano material can be used to detect hydrogen peroxide with a low concentration. The absorbance of catalytic product and the concentration (0.01-0.1 mmol/L) of hydrogen peroxide are in a linear relationship, and the detection limit is 0.0025 mmol/L.

The invention discloses a preparation method of a low-cost and high-sensitivity fluorescent/colorimetric dual-mode sensor for visual miRNA detection. The sensor is successfully used for miRNA detection. A paper chip is prepared by utilizing a wax printing technology, platinum nanoparticles grow in a working area of a fluorescent layer, the quenching effect of graphite-phase carbon nitride nanosheets on palladium nanoclusters and duplex specific nuclease are utilized to achieve nucleic acid circulation signal amplification, and accordingly accurate measurement of small molecule miRNA is achieved. The prepared paper chip is folded, and visual predetermination can be achieved by dropwise adding a color developing solution in a reaction area; then the paper chip is placed in a fluorescent dishto achieve accurate fluorescent miRNA determination. The operation is simple, convenient and fast, time and effort are saved, and a new reliable method is provided for miRNA detection.

The invention relates to an immune nutrition composition and application thereof. The immune nutrition composition disclosed by the invention comprises the following components in parts by weight: 45-50 parts of hydrolyzed small molecular protein, 10-15 parts of water-soluble dietary fiber, 20-25 parts of edible mushrooms extract, 2-5 parts of selenium-enriched yeast, 5-10 parts of fructo oligosaccharides and 0.63-1.8 parts of vitamin. The immune nutrition composition disclosed by the invention can be used in cancer therapy. According to the immune nutrition composition disclosed by the invention, the cancer chemotherapy and radiotherapy tolerance can be improved, and the capability of killing tumor cells by CIK cells can be improved.

The invention discloses tungsten-oxide-quantum-dot-material simulated peroxidase. A tungsten oxide quantum dot material has excellent peroxidase activity and can catalyze color developing agents such as hydrogen-peroxide-oxidized 3,3',5,5'-tetramethyl benzidine hydrochloride, diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) and o-phenylenediamine to develop colors. In this way, a novel simulated peroxidase inorganic nano-material capable of replacing natural enzymes is provided. The tungsten oxide quantum dot material is prepared in the mode that tungsten sulfide serves as a precursor, and reacting is carried out through a simple ultrasonic step and a hydrothermal method. The material is simple in synthesis step, environmentally friendly and low in cost; in addition, the peroxidase simulating effect is obvious, and therefore good application prospects are achieved in novel catalytic oxidation and medical analysis.

The invention relates to a traditional Chinese medicine for reducing the blood fat, and a preparation method thereof. The traditional Chinese medicine comprises: a dry extract paste of extracts obtained through carrying out water decoction extraction or ethanol reflux extraction on mixed Rhizoma Alismatis, haw, Gynostemma pentaphylla, Radix Puerariae and Lycium Chinense; and Panax Notoginseng powder. The preparation method comprises the following steps: taking and mixing the Rhizoma Alismatis, the haw, the Gynostemma pentaphylla, the Radix Puerariae and the Lycium Chinense, extracting through decocting with water or refluxing with ethanol to obtain an extract solution, and concentrating the extract solution to obtain the dry extract paste; selecting Panax Notoginseng, drying, carrying out radiant quantity disinfection, and crushing to 80 mesh fine powder; and adding the water or ethanol extraction dry extract paste and the Panax Notoginseng powder to a mixer together, and mixing to obtain the traditional Chinese medicinal powder for reducing the blood fat of the invention. The obtained medicinal powder can be filled to capsules or added with other routine medicinal carriers or excipients to prepare usual oral medicinal forms. Hyperlipidemia belongs to the 'turbid phlegm' category of the traditional Chinese medicine, so the traditional Chinese medicine screens the composition according to dampness removing, turbidity transforming, stasis scattering and vein relaxing principles, can nourish blood in the liver and the kidney, and also can prevent extremeness disadvantages of the haw and the Gynostemma pentaphylla, thereby the new traditional Chinese medicine with a substantial curative effect is provided for treating the hyperlipidemia.

The invention discloses a method for preparing active porous nano cerium dioxide based on an organic template. The method comprises the following steps: adopting 2-methylimidazole as a template material, controlling a calcination atmosphere and a calcinations temperature, synthesizing porous CeO2 nano particles with uniform appearance. The CeO2 nano particles obtained by the invention have the characteristics of small size, small particle size and the like, have good peroxidase activity, have the advantages of simple preparation method, low preparation cost and the like, and facilitates the large-scale popularization.

The invention discloses an iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme as well as a preparation method and application thereof. The invention belongs to the field of nanotechnology and catalytic materials. The structural formula of the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme is K10P2W18Fe4 (H2O) 2O68/PDA, and the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme is obtained by preparing a precursor Na8 [HPW9O34], preparing K10P2W18Fe4 (H2O) 2O68 and preparing the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme. The material disclosed by the invention has a catalytic function of peroxide mimic enzyme and can be used as novel peroxide mimic enzyme; the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme can replace horse radish peroxidase to be applied to immunoassay, biological detection and clinical diagnosis; in addition, the iron-substituted tungsten phosphate polydopamine nano mimic enzyme prepared by the invention can be used for colorimetric or fluorescent detection of hydrogen peroxide, glutathione and escherichia coli, and thedetection sensitivity is relatively good.

The invention provides a novel method for disinfecting a wound through a gold nanocluster-graphene composite (AuNCs-Go) and ascorbic acid (AA) based adhesive bandage. The gold nanocluster-graphene composite is high in near-infrared ray (NIR) absorption and peroxidase activity, and can effectively catalyze a high-antibacterial activity hydroxyl radical produced in the decomposition of hydrogen peroxide produced by the prodrug ascorbic acid, so that the toxicity of the high-concentration hydrogen peroxide is avoided. The adhesive bandage has the chemotherapy/photo-thermal synergic treatment effects, and a designed antibacterial system has a broad spectrum and an efficient antibacterial activity, so that the healing of the wound is accelerated.

The invention discloses a preparation method of a flower-like silver nanozyme with peroxidase activity and belongs to the technical field of preparation of nano materials. Under the condition of continuous stirring, an aqueous solution of histidine with pH of 11.2-11.8 is mixed with a silver nitrate solution, and then a reducing agent, a hydroxylamine solution is added; and after the reaction lasts for 5 minutes, the flower-like silver nanozyme with good monodispersity is obtained. By changing the pH value of the histidine, the flower branch length of the product can be adjusted, and by changing the final concentration of the histidine, the size of the product can be adjusted. The preparation method is easy to operate; by changing the reaction conditions, the flower-like silver nanozyme ofdifferent sizes and different flower branch lengths can be prepared; and the prepared flower-like silver nanozyme has good monodispersity and extremely-high peroxidase activity, and can better meet the needs of practical application.

The invention discloses a method for preparing active porous nano-cerium dioxide based on an inorganic template. Ammonium bicarbonate serves as a template material and is mixed with cerium nitrate, and then the template material is removed by high-temperature annealing to obtain porous CeO2 nanoparticles. The obtained CeO2 nanoparticles have the advantages of small size and particle size and the like, and have good peroxidase activity. The preparation method of the CeO2 nanoparticles has the advantages of simplicity, low preparation cost and the like, and large-scale popularization is benefited.

The invention belongs to the technical field of food, and particularly relates to a grain amaranth drink and a preparation method thereof. The preparation method comprises the following steps: by taking amaranth, oats, wheat, mung beans, soybeans, fructose oligosaccharide and a stabilizing agent as active ingredients, selecting the raw materials, roasting and grinding, blending and marking up to the volume, homogenizing at high pressure, performing ultrahigh-temperature instantaneous sterilization, and filling to obtain the grain amaranth drink. The grain amaranth drink has balanced nutrients, and is high in nutrition potency, can be filled with sterility, can be industrially produced, and has the advantages of being long in shelf life, easy to carry, ready to drink once opening, convenient to drink and suitable for drinking of people with affluenza, and the like.

The invention discloses a preparation method of phthalocyanin modified cerium dioxide nano particles. The preparation method comprises the steps of selecting phthalocyanin and cerous nitrate hexahydrate as raw materials, dissolving the cerous nitrate hexahydrate in deionized water to obtain a clear solution A, dissolving the phthalocyanin in deionized water to obtain a solution B and regulating the pH value of the solution B to the range of 7.5-8.5, mixing the clear solution A with the solution B, stirring evenly to obtain a mixed solution, injecting H2O2 to the mixed solution, stirring evenly, and then transferring to the reaction kettle for a hydrothermal reaction, cooling to a room temperature after the reaction is completed, and then washing and drying to obtain the phthalocyanin modified cerium dioxide nano particles. According to the preparation method, the cerium dioxide is modified by use of the phthalocyanin; the obtained phthalocyanin modified cerium dioxide nano particles have the characteristics of high purity, small size, even particle size, good peroxidase activity and the like, and also has excellent stability and selectivity.

The invention belongs to the technical field of planting of dendrobium officinale and particularly discloses a fertilizer application method capable of controlling diseases and pests of dendrobium officinale. The specific method comprises steps as follows: after seedlings of dendrobium officinale are transplanted for 14-16 days, a nutrient solution is started to be sprayed on leaves and then sprayed once every other 34-36 days; after the seedlings of dendrobium officinale are transplanted for 27-29 days, a fertilizer liquid is applied to roots and then applied once every other 45-47 days; growth demands of dendrobium officinale are effectively met, besides, the dosage of fertilizer is limited to keep certain stress conditions, the resistance is improved, germs and pests are effectively killed, diseases and pests are inhibited, the root system activity is effectively enhanced, improvement of chlorophyll synthesis is promoted, the activity of peroxidase of dendrobium officinale is improved, peroxides in bodies are cleared, growth and accumulation of bioactive substances are promoted, synthesis and accumulation of effective ingredients such as polysaccharide, alkaloid and the like are promoted, and the medical value of dendrobium officinale is increased effectively.

The invention discloses a planting method for improving nutritional values of tomato, and belongs to the technical field of tomato planting. The planting method comprises the following steps: (1) selecting seeds; (2) performing cleaning and disinfection treatments; (3) performing seed soaking treatments; (4) performing arc magnetized plasma treatments; (5) performing ultraviolet curing treatments;(6) performing drying treatments; (7) performing sowing; (8) performing light treatments; and (9) performing transplantation. Tomato fruits planted by the planting method are plump and full, bright in color, high in yield and good in quality. Tomato contains rich proteins, reducing sugars, vitamins, lycopene and other nutrients, and has good nutritional values. In addition, tomato has strong disease resistance and strong growth. The planting method has an extremely great market popularization value.

The invention provides DNA functionalized copper oxide nano-enzyme as well as a preparation method and application thereof. The DNA functionalized copper oxide nano-enzyme comprises di-DNA and CuO nano-enzyme, one end of the di-DNA is a DNA sequence composed of TC bases, the other end is a DNA sequence composed of nucleoside, and the di-DNA is adsorbed on the CuO nano-enzyme through the DNA sequence composed of the TC bases. The DNA functionalized copper oxide nano-enzyme is designed on the basis of an adsorption mechanism of DNA on the surface of the copper oxide nano-enzyme, and the DNA canbe polarized and adsorbed on the surface of the nano-enzyme and endows the nano-enzyme with a tumor targeting function. The preparation method comprises the following steps: dispersing di-DNA into a buffer solution to obtain a dispersion solution; and mixing the dispersion solution with CuO nano-enzyme, and performing adsorbing. The preparation method disclosed by the invention is simple to operate, low in cost and mild in reaction conditions, can be used for tumor targeted modification of the nano-enzyme, and has a wide application prospect in the fields of disease treatment and the like.

The invention relates to the technical field of biology, in particular to lyceum ruthenicum fermentation original liquor and a preparation method thereof. The method is characterized in that an initial system obtained by mixing of lyceum ruthenicum dried powder, water and bacterial liquid is fermented to obtain fermentation liquor, and the fermentation liquor is sterilized and centrifuged to obtain supernatant. Compared with the prior art, the lyceum ruthenicum fermentation original liquor and the preparation method thereof have advantages that plant activity in products is kept to the greatest extent, adding of any organic agents in preparation is avoided, fermentation temperature and fermentation pH are mild, damages of plant active component structures are avoided, natural plant activity is kept, and adding of chemical components such as essences in the fermentation original liquor is avoided, so that safety of the products to human bodies is guaranteed.