10results about How to "Increased peroxidase activity" patented technology

The invention relates to the technical field of nano materials, in particular to a ferrocerium bimetal organic framework derivative as well as a preparation method and an application method thereof. The preparation method of the ferrocerium bimetal organic framework derivative provided by the embodiment of the invention comprises the following steps: S1, dissolving cerium salt and iron salt in a solvent to obtain a first solution; s2, dissolving an organic matter containing a benzene ring in a solvent to obtain a second solution; s3, mixing the first solution and the second solution, and adjusting to an alkaline environment to initiate a reaction to obtain a cerium-iron bimetal framework; and S4, carrying out heat treatment on the cerium-iron bimetal framework to obtain the cerium-iron oxide. The embodiment of the invention provides a ferrocerium bimetal organic framework derivative as well as a preparation method and an application method thereof, and can provide a material capable of increasing the catalytic efficiency and enhancing the coordination capability of ATP (adenosine triphosphate), and the material is used for treating melanoma.

The invention relates to an MOF-DNA composite material as well as a preparation method and application thereof. The MOF-DNA composite material comprises a metal-porphyrin derivative organic framework structure, and G-DNA is compounded on the metal-porphyrin derivative organic framework structure; wherein the porphyrin derivative is a porphyrin derivative containing Fe. The MOF-DNA composite material disclosed by the invention has very excellent peroxidase activity, and a metal-porphyrin derivative organic frame structure and G-DNA have a mutual synergistic effect, so that the peroxidase activity can be greatly enhanced.

The invention provides a polyvinylpyrrolidone modified cerium-based UIO-66 nano material as well as a preparation method and application thereof, and relates to the technical field of nano materials, catalysts and antitumor drugs. According to the method, cerium ions are used as metal nodes, terephthalic acid is used as an organic connector, a crystal framework with a UIO-66 characteristic topological structure is formed, then, polyvinylpyrrolidone is introduced into framework nano particles in situ through solvothermal reaction, and the nano particles are prepared. Therefore, the polyvinylpyrrolidone modified cerium-based UIO-66 nano material which is uniform in morphology and good in dispersity and has an efficient piezoelectric effect and peroxidase activity is obtained. The cerium-based UIO-66 nano-material disclosed by the invention can obviously enhance the catalytic performance under remote and accurate regulation and control of ultrasonic waves, and excites an additional biological catalytic function, so that the defect of single treatment is overcome through multi-mechanism synergy, and high-efficiency, low-toxicity and controllable treatment on tumors is realized.

The application belongs to the technical field of chemical analysis and detection, and relates to a synthesis method of an MXene-based nanoenzyme composite and application of the MXene-based nanoenzyme composite in detection of organophosphorus pesticides. V2CTx MXene and Fe-TCPP can be used to prepare V-TCPP(Fe) materials through a simple solvothermal method, and V-TCPP(Fe), AChE, 2-methylimidazole and zinc nitrate can be stirred at room temperature to prepare V-TCPP(Fe) / AChE@ZIF-8 materials. PVA, sucrose and V-TCPP(Fe) / AChE@ZIF-8 can be mixed and stirred to prepare a hydrogel patch. The material has a good colorimetric / chemiluminescence linear range response to OPs. The material is simple and convenient to prepare, and has the advantages of high sensitivity, a large linear range, good stability and repeatability in detection of OPs.

The invention discloses application of PvFIP37 protein and related biological materials thereof in regulation and control of cadmium tolerance of plants. According to the invention, the PvFIP37 gene is cloned from switchgrass, and the PvFIP37 overexpressed switchgrass is successfully constructed. The analysis on the phenotype of the PvFIP37 overexpressed switchgrass under cadmium stress finds that compared with wild type switchgrass, the cadmium tolerance of the switchgrass can be enhanced by increasing the expression quantity of the PvFIP37 gene, which indicates that the PvFIP37 has the function of regulating and controlling the cadmium tolerance of plants. The switchgrass PvFIP37 gene provided by the invention can be used as an excellent gene resource, is widely applied to the field of genetic breeding of gramineous grass or other crops, and has important significance on understanding of tolerance of plants under heavy metal stress.

The invention discloses a pain photodynamic therapy instrument, comprising a treatment bed, an operation panel and a multi-axis support structure, wherein the operation panel is fixedly installed on one side of the treatment bed, the multi-axis support structure is fixedly installed at the middle position of one end of the treatment bed, a mounting plate is rotatably installed on one side of the top of the multi-axis support structure, an MCU processor is fixedly installed on the inner top of the mounting plate, a plurality of groups of red lasers and infrared lasers are fixedly installed on the inner bottom of the mounting plate, and the output end of each group of red lasers and infrared lasers is fixedly connected with an optical fiber, and the bottom end of each group of the optical fibers is fixedly installed with an optical fiber connection needle; the pain photodynamic therapy instrument avoids the problem that the existing single group of colored light irradiation can only play a single therapeutic effect during treatment and is often difficult to play a good anti-inflammatory and analgesic effect by providing the MCU processor, red laser, infrared laser, optical fiber and optical fiber connection needle.

The invention discloses application of a blueberry VcDELLA-1 gene in improving high temperature stress resistance of plants, and belongs to the field of plant genetic engineering. Based on blueberry heat stress transcriptome data analysis, it is found that only VcDELLA-1 in a DELLA family contains a DLPYAD motif, the expression quantity of the gene in a salicylic acid treatment group is remarkably higher than that of a control group, and it is prompted that the gene is the blueberry heat-resistant key gene. Under high temperature and salicylic acid treatment, VcDELLA-1 expression in VcDELLA-1 transgenic arabidopsis thaliana and blueberry plants is remarkably up-regulated, and the curling and shrinking degree of leaves is relatively light; the activity of superoxide dismutase is remarkably reduced, the activity of peroxidase and the content of malondialdehyde and hydrogen peroxide are remarkably increased, and a large number of heat stress related genes are expressed. The VcDELLA-1 gene improves the high-temperature resistance of plants by regulating and controlling the expression of heat stress related genes, and provides important gene resources for cultivating new varieties of high-temperature-resistant plants.

The application relates to a preparation method of a nanometer enzyme detection reagent for metronidazole residues in apples, and belongs to the technical field of pesticide residues and food safety. The specific operation is as follows: (1) mesoporous polydopamine powder is prepared by using polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, dopamine hydrochloride and the like; (2) a copper-manganese bimetal carbon dot solution is prepared by using citric acid, copper chloride, manganese chloride and ethylenediamine; and (3) a nanometer enzyme detection reagent is prepared by using the mesoporous polydopamine solution and the copper-manganese bimetal carbon dot solution. The mesoporous polydopamine has a large specific surface area, can provide a large number of positions for the loading of carbon dots, and can well adsorb metronidazole molecules. The copper-manganese bimetal carbon dots make the nanometer enzyme detection reagent have good enzyme-like activity, and generate pesticide concentration-dependent colorimetric signals and photothermal signals. The application realizes mutual checking of double-mode output signals, and significantly improves the reliability of detection; and the detection is completed within 1.5 hours.

The invention provides a multi-interface heterojunction high-entropy sulfide, a preparation method and application thereof, and a chloramphenicol concentration detection method. According to the preparation method disclosed by the invention, (CdZnCoNiMn) Sx and PtRuFeCoMn high-entropy alloy nano-enzymes are prepared by adopting a solvothermal method and an oil bath method; wherein the (CdZnCoNiMn) Sx has optical activity, and the high-entropy alloy nano enzyme shows excellent peroxidase activity. Due to the fact that multiple different metal elements in the (CdZnCoNiMn) Sx are embedded into crystal lattices, the crystal lattice distortion effect is caused, and the photoelectric activity of the material is remarkably improved; and further, signal amplification is successfully realized through biological catalytic precipitation catalyzed by the high-entropy alloy nano-enzyme. The system shows ultrahigh sensitivity and linear response characteristics in chloramphenicol concentration detection, chloramphenicol can be sensitively detected within the range of 0.001-100 ng mL <-1 >, and the detection limit is as low as 0.7454 pg mL <-1 >.

The invention relates to a method for preventing and treating grape white rot, which is characterized in that a thiamine solution is uniformly sprayed on grape leaf surfaces, so that the disease resistance of grapes can be enhanced, the grapes can be induced to generate defense, including enhancing the activity of phenylalanine ammonialyase and peroxidase, and increasing the content of hydrogen peroxide, lignin, total phenols, flavonoids, salicylic acid and jasmonic acid in leaves; therefore, the grape white rot can be well prevented and treated. According to the method, the grape plants are subjected to thiamine solution pretreatment by utilizing a specific system defense mechanism of the plants, so that the grape plants can be induced to generate defense, and the method has an important application value in the aspects of grape disease prevention and the like.