Iridium nano-enzyme having activity of tandem enzyme and application thereof

A nano-enzyme and enzyme activity technology, applied in the field of analytical chemistry, can solve unreported problems

Active Publication Date: 2019-01-25
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzyme-free spectrophotometric methods based on single noble metal nanozymes are rarely reported
[0006] In summary, Ir nanozymes with glucose oxidase, tandem enzyme activity are novel in related fields, and no enzyme-free one-pot glucose spectrophotometric detection based on Ir nanozyme with tandem enzyme activity has been reported yet.

Method used

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  • Iridium nano-enzyme having activity of tandem enzyme and application thereof
  • Iridium nano-enzyme having activity of tandem enzyme and application thereof
  • Iridium nano-enzyme having activity of tandem enzyme and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Ir nanozyme:

[0031] IrCl 3 (8.4 mmol / L, 4 mL) was added dropwise to a vigorously stirred ethanol solution containing polyvinyl alcohol (18.6 mg, 4 mL). Then stirred at room temperature for 12 h to obtain a clear light yellow solution, and then refluxed at 100°C for 6 h. The resulting brown solution was evaporated to completely remove the solvent, and the black solid obtained by evaporation was the Ir nanozyme. figure 1 is the TEM image of the Ir nanozyme observed by transmission electron microscopy.

Embodiment 2

[0033] Verification of imitation peroxidase activity of Ir nanozyme:

[0034] Experimental system a: The catalytic reaction system contains H 2 o 2 (1.2 mmol / L), Ir nanozyme (40 µg / mL) obtained in the above example, organic chromogen TMB (0.6 mmol / L) and acetate buffer (pH 4.0, 100 mmol / L). After reacting at room temperature (25°C) for 30 minutes, use a UV spectrophotometer to detect its absorbance within 500−800 nm;

[0035] In addition, two control experiments were done: in the catalytic reaction system of one of the control experiments b, Ir nanozyme was not added, and the absorbance was detected after 30 minutes of reaction under the same conditions as the above-mentioned experimental system; the catalytic reaction system of the other control experiment c was Ir nanozyme. Nanozyme (40 µg / mL) and acetate buffer (pH 4.0, 100 mmol / L), after standing for 30 minutes under the same conditions as the above experimental system, the absorbance value was detected;

[0036] Such a...

Embodiment 3

[0038] Verification of the imitation glucose oxidase activity of Ir nanozyme:

[0039]Experimental system a: The catalytic reaction system is composed of glucose (1.2 mmol / L), Ir nanozyme (40 µg / mL) obtained in the above example and acetate buffer (pH 4.0, 10 mmol / L), at room temperature (25 ℃ ) after 30 minutes of reaction, centrifuge to get the supernatant. Add organic chromogen TMB (0.6 mmol / L), HRP (20 µg / mL) and acetate buffer (pH4.0, 100 mmol / L) to the supernatant. After continuing to react at room temperature (25 °C) for 30 minutes, use a UV spectrophotometer to detect its absorbance within 500−800 nm;

[0040] Two other control experiments were done: the catalytic reaction system of one control experiment b did not add Ir nanozyme, and its absorbance was detected after the reaction under the same conditions; the catalytic reaction system of the other control experiment c included Ir nanozyme (40 µg / mL) and acetate buffer (pH 4.0, 100 mmol / L), the absorbance was dete...

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Abstract

The invention relates to the field of nanometer materials, catalysis and analysis, in particular to iridium nano-enzyme having activity of tandem enzyme and application thereof. The iridium nano-enzyme has the activity of imitated peroxidase and the activity of imitated glucose oxidase, and the activity of imitated enzymes is similar to an optimum active pH. The iridium nano-enzyme is utilized toachieve one-pot non-enzyme developing glucose detection and is hopeful to be widely used in the fields such as analytical chemistry, biological medicine, food engineering and catalysis.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and specifically includes an iridium nanozyme with tandem enzyme activity and application thereof. Background technique [0002] Nanozyme, also known as nanomimetic enzyme, generally refers to nanomaterials with enzymatic activity, and is a kind of artificial enzyme. In recent years, researchers have developed nanozymes into highly stable and inexpensive natural enzyme substitutes and successfully applied them to a series of reactions. Tandem enzymes are multifunctional enzymes in organisms, which can have multiple catalytic functions at the same time, and the catalytic functions are cascaded. Since the reported nanozymes usually have only one imitation enzyme activity, such as catalase activity, peroxidase activity, glucose oxidase activity or superoxidase activity, and nanozymes with glucose oxidase activity Less reported. Therefore, it is of great significance to find new nanomaterials w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/3103G01N21/78
Inventor 韩磊梁欣李峰
Owner QINGDAO AGRI UNIV
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