Palladium nano-particles with peroxidase activity and preparation method thereof

A palladium nanoparticle, peroxidase technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem of low peroxidase activity, difficult to control conditions, and complicated preparation. and other problems, to achieve the effect of simple method, easy control of conditions and high catalytic activity

Active Publication Date: 2016-05-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its preparation is cumbersome, the cycle is long, the conditio

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  • Palladium nano-particles with peroxidase activity and preparation method thereof
  • Palladium nano-particles with peroxidase activity and preparation method thereof
  • Palladium nano-particles with peroxidase activity and preparation method thereof

Examples

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Embodiment 1

[0035] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0036] (1) Deoxynucleotide C 10 The annealing of:

[0037] Dissolve 25 μL of 20 μM deoxynucleotide C 10 Add aqueous solution, 100 μL of 10 mM sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution with a pH of 5.0 to a 1.5 mL centrifuge tube and add triple distilled water to make the total volume 400 μL, mix well; heat up to 95 °C, maintain for 5 min, and cool down to room temperature;

[0038] (2) Preparation of palladium nanoparticles:

[0039] Add 12 μL of 300 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 25°C, let it stand for 2 hours, add 15 μL of 1.5mM dimethylamine borane aqueous solution, mix well, and After standing at 25°C for 12 hours, a palladium nanoparticle liquid with peroxidase activity was obtained. The average particle size obtained by scanning with a tr...

Embodiment 2

[0047] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0048] (1) Deoxynucleotide G 200 The annealing of:

[0049] 2.5 μL of 2 μM deoxynucleotide G 200 Add aqueous solution, 50 μL 5 mM potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a pH of 3.0 to a 1.5 mL centrifuge tube, add triple distilled water to make the total volume 400 μL, mix well; heat up to 90 °C, maintain for 10 min, and cool down to room temperature;

[0050] (2) Preparation of palladium nanoparticles:

[0051] Add 8 μL of 200 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 20°C, let it stand for 4 hours, add 6 μL of 0.6mM sodium borohydride aqueous solution, mix well, and heat at 20°C Stand still for 15 hours to obtain a palladium nanoparticle liquid with peroxidase activity.

[0052] The average particle size obtained by scanning with a transmi...

Embodiment 3

[0054] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0055] (1) Deoxynucleotide C 200 The annealing of:

[0056] Dissolve 250 μL of 200 μM deoxynucleotide C 200Add aqueous solution, 200 μL 20 mM sodium dihydrogen phosphate-phosphoric acid solution with a pH of 9.0 to a 1.5 mL centrifuge tube and add triple distilled water to make the total volume 400 μL, mix well; heat up to 100 °C, maintain for 5 min, and cool down to room temperature;

[0057] (2) Preparation of palladium nanoparticles:

[0058] Add 16 μL of 400 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 30° C., let stand for 0.5 h, add 24 μL 2.4 mM dimethylamine borane aqueous solution, mix well, Standing at 30° C. for 10 h, a palladium nanoparticle liquid with peroxidase activity was obtained.

[0059] The average particle size obtained by scanning with a transmission electron micros...

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Abstract

The invention discloses palladium nano-particles with peroxidase activity and a preparation method thereof. The preparation method includes the following steps that (1) annealing of deoxynucleotide is conducted, specifically, a deoxynucleotide aqueous solution and a phosphate buffered solution are added into a 1.5 mL centrifuge tube and mixed evenly, and the temperature is increased to 90-100 DEG C, maintained for 5-10 min, and decreased to the room temperature; and (2) the nano-particles are prepared, specifically, a sodium tetrachloropalladate aqueous solution is added to the annealed deoxynucleotide aqueous solution obtained in the step (1), the mixture is mixed evenly and stands, a reducing agent aqueous solution is added, the mixture is mixed evenly and stands, and liquid of the palladium nano-particles with peroxidase activity is obtained. The method disclosed by the invention is simple, the period is short, conditions are easy to control and mild, and large-scale industrial production and preparation are facilitated. The mean grain size of the nano-particles with peroxidase activity is 1.0-4.8 nm, and the nano-particles with peroxidase activity is high in catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle preparation, and in particular relates to a preparation method of palladium nanoparticle with peroxidase activity. Background technique [0002] Palladium nanomaterials have been recognized as a highly ideal catalyst, and its catalytic activity has a great relationship with surface geometric effects, electronic properties and quantum size effects. So far, palladium nanoparticles have been prepared with carbon, metal oxides, dendrimers, block copolymer micelles, mesoporous molecular sieves, etc. as support materials. Its preparation is cumbersome, the cycle is long, the conditions are not easy to control, and the activity of peroxidase is low. Therefore, it is of great practical significance to develop a convenient and effective preparation method of palladium nanoparticles with peroxidase activity. Contents of the invention [0003] The purpose of the present invention is to provide a met...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B01J23/44B01J35/00B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00B01J23/44B01J35/006B22F9/24B22F1/054
Inventor 付雁李韡张金利王雨智星
Owner TIANJIN UNIV
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