Method for rapid identification of heat-resistance seedling state of cotton

An identification method, heat resistance technology, applied in the direction of botanical equipment and methods, horticulture, application, etc., can solve problems such as long time span

Inactive Publication Date: 2012-07-18
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the survey of pollen-related traits can be carried out in July, while the

Method used

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  • Method for rapid identification of heat-resistance seedling state of cotton
  • Method for rapid identification of heat-resistance seedling state of cotton
  • Method for rapid identification of heat-resistance seedling state of cotton

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Screening of heat-resistant materials

[0022] The correlation between shedding rate and pollen germination rate in different plants has been shown to be useful as an indicator of heat tolerance in cotton (Prasad et al. 1999; Aloni et al. 2001; Reddy et al. 1999).

[0023] In 2007, 200 copies of different cotton germplasm resources or varieties were planted in Turpan, Xinjiang (the growth period is usually above 35°C, and sometimes even as high as above 40°C). The degree of wilting, sterile seed rate, pollen morphology, pollen vigor and other traits were used to identify the heat-resistant characteristics of different cottons.

[0024] In order to exclude the impact of continuous high temperature in the field on the in vitro culture of pollen, find out the best field identification heat resistance index. First analyze the height of the shedding rate of 29 sensitive and heat-resistant materials (shedding rate > 60% is sensitive to high temperature, shedding ra...

Embodiment 2

[0031] The screening of embodiment 2 cotton growth period and adversity condition

[0032] Cotton seedlings in six different stages of the cotton seedling stage, namely: cotyledon stage, one-leaf stage, two-leaf stage, three-leaf stage, four-leaf stage and five-leaf stage, were subjected to control (30°C), 35°C, 40°C, After treating the heat-resistant materials Changkang cotton and Nandanbadi Dahua selected in Example 1 at 45°C and 48°C, and the high-temperature sensitive materials Daizimian 55 and Zaoshu Changrong No. 7 for 8 hours, the ascorbic acid peroxidation was measured. The evaluation indexes of biomolecules (APX) and relative conductivity are shown in Table 2.

[0033] Nandanbadi Dahua and Changkang cotton showed strong heat resistance in each period, and the heat resistance of Nandanbadi Dahua was stronger than Changkang cotton. At the cotyledon stage, the heat resistance of each material has little difference and cannot be used as an identification index. At the f...

Embodiment 3

[0055] Plant 15 different heat-resistant materials in nutrient pots (0.03m2 in size) under normal conditions, with 5 plants in each pot, plant three times for each material, and grow to the three-leaf stage (three unfolded true leaves). The seedlings at the three-leaf stage were placed in a light-temperature incubator at 40° C. for 8 hours (relative humidity was 70%); at the same time, the control of corresponding materials was set: the seedlings were placed in a light-temperature incubator at 30° C. for 8 hours, and the relative humidity was 70%. Fresh leaves (all taken) were taken from the treatment and control materials at the same time for the determination of ascorbate peroxidase (APX) activity content, relative conductivity and seedling wilting rate, using its relative value P [P=(X-Y) / Y; under X stress The measured value of Y is the control measured value under normal conditions] as an evaluation index.

[0056]It can be seen from Table 3 that there are significant or e...

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Abstract

The invention provides a method for rapid identification of a heat-resistance seedling state of cotton, which comprises the following steps of: treating cotton seedlings at trefoil stage to quatrefoil stage for 5-12 hours at the temperature of 40-45 DEG C; setting comparison under normal growth condition; respectively determining ascorbic acid peroxidase activity, relative electric conductivity and seedling withering rate of treated cotton seedlings and normal growing cotton seedlings; calculating relative values of the ascorbic acid peroxidase activity, the relative electric conductivity and the seedling withering rate relative to those of the normal growing cotton seedlings; and determining heat resistance. The method can be used for effectively identifying the heat resistance of different cotton germplasm resources rapidly, and can provide appropriate parent materials for high-temperature resistant seed breeding.

Description

technical field [0001] The invention relates to heat-resistant identification of plants, in particular to a rapid identification method for cotton heat-resistant seedlings. Background technique [0002] With global warming, heat stress caused by rising temperatures has affected agriculture in many countries and regions (Hall, 2001). Emissions of gases from human activities have increased the concentration of greenhouse gases, especially carbon dioxide, methane, chlorofluorocarbons and nitric oxide. These greenhouse gases will gradually increase the average ambient temperature. According to the report of the Intergovernmental Symposium on Climate Change, the global average temperature will increase by 0.3 degrees per decade, leading to global warming (Jones et al., 1999). The continuously rising temperature may change the geographical distribution and growing season of crops, and advance development and maturity to avoid the extreme high temperature for growth and developmen...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 杜雄明刘少卿孙君灵何守朴贾银华周忠丽潘兆娥王立如庞保印
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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