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N-doped ferroferric oxide peroxide mimic enzyme and glucose detection method thereof

A technology of iron ferric oxide peroxide and imitation enzyme, which is applied in the field of analytical chemistry, can solve the problems of low affinity of nano-imitation enzyme substrate, achieve low detection limit, wide linear range, and improve catalytic efficiency

Inactive Publication Date: 2019-08-23
SHANDONG INST FOR PROD QUALITY INSPECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Fe 3 o 4 Nanomimetic enzymes show HRP-like activity under acidic conditions, but unmodified Fe 3 o 4 Nanomimetic enzymes still have low affinity for substrates

Method used

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  • N-doped ferroferric oxide peroxide mimic enzyme and glucose detection method thereof
  • N-doped ferroferric oxide peroxide mimic enzyme and glucose detection method thereof
  • N-doped ferroferric oxide peroxide mimic enzyme and glucose detection method thereof

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Embodiment 1

[0035] The preparation process of the nitrogen-doped iron ferric oxide peroxide mimetic enzyme involved in this embodiment comprises the following steps:

[0036] (1) Take ferric chloride hexahydrate, urea, polyethylene glycol and ethylene glycol by weight components; Ferric chloride hexahydrate, urea, polyethylene glycol are added in ethylene glycol, stir and mix well, then Add it to the hydrothermal synthesis reaction kettle, and place it in a dry oven at 240°C for 16 hours;

[0037] (2) Then the product after the reaction of step (1) is transferred to a beaker filled with absolute ethanol solution, and the synthesized particles are dispersed in the absolute ethanol solution by ultrasonic for 6 minutes, then magnetically separated, discarded remove the supernatant;

[0038] (3) Wash the product discarded from the supernatant in step (2) with deionized water for 3 times, and redisperse it with deionized water and store it to obtain Fe with different concentrations of different...

Embodiment 2

[0047] A method for detecting glucose by nitrogen-doped iron ferric oxide peroxide mimic enzyme, the step process is as follows:

[0048] (1) Pretreatment: prepare 100mL glucose stock solution, and pretreat the glucose solution by centrifugation and ultrafiltration;

[0049] (2) Dilution: Use pH 4.0 acetate buffer system to dilute the pretreated glucose solution to 5×10 -4 mol / L;

[0050] (3) Water bath: take 50 μL of the glucose solution diluted in step (2), add it to 10 mL of phosphate buffer with a pH of 7.0, add 5 μL of 40 mg / mL glucose oxidase, and bathe in water at 37° C. for 30 minutes to obtain a reaction solution;

[0051] (4) Determination: Take 30 μL of reaction solution in 2.4 mL of acetate buffer system with pH 4.0, then add 120 μL of tetramethylbenzidine (TMB) with a concentration of 10 mg / mL, 240 μL of N-Fe with a concentration of 2 mg / mL 3 o 4 The nano-mimetic enzyme was mixed evenly and placed in a water bath at 45°C for 20 minutes, and the absorbance value...

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Abstract

The invention belongs to the technical field of analytical chemistry, and in particular relates to a nitrogen-doped ferroferric oxide peroxide mimic enzyme and a glucose detection method thereof. Nitrogen substance urea modification is performed on Fe3O4 nanometer mimic enzyme to synthesize N-Fe3O4 nanometer mimic enzyme and research the catalytic activity of the Fe3O4 nanometer mimic enzyme; compared with other modifiers, due to the introduction of nitrogen, the peroxidase activity and catalytic efficiency of the Fe3O4 nanometer mimic enzyme can be significantly improved; a stable hydrogen bond is formed between -NH2 of the Fe3O4 nanometer mimic enzyme and H2O2, and thus the appetency with the H2O2 is improved; by using the characteristic of catalyzing H2O2, in combination with the glucose oxidase that catalytically oxidizes glucose to generate H2O2, the method for quickly detecting the glucose content is built; compared with the prior art, the method has lower detection limit, widerlinear range and better specificity, and the better method is provided for measuring the glucose content in the actual sample.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a nitrogen-doped iron ferric oxide peroxide mimic enzyme and a method for detecting glucose. Background technique [0002] Biological enzymes are biological macromolecules with high specificity and catalytic activity, which can improve the efficiency of chemical reactions. Most biological enzymes are proteins, which have disadvantages such as poor stability, difficulty in purification, and easy inactivation, which limit their wide application. Therefore, the development of materials with good stability, high catalytic activity and low cost with biological enzyme catalytic activity has gradually become a research hotspot. Nanomaterials with catalytic activity that mimic enzymes are called nanomimetic enzymes. In 2007, the first report of ferroferric oxide (Ferroferric oxide, Fe 3 o 4 ) Magnetic nanoparticles have horseradish peroxidase (Horseradish Pero...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28G01N1/38B01J19/00C01G49/08B82Y30/00B82Y40/00
CPCB01J19/0006B82Y30/00B82Y40/00C01G49/08G01N1/28G01N1/38G01N21/31G01N2001/386
Inventor 邴欣贾敏贺祥柯沙隽伊李志华
Owner SHANDONG INST FOR PROD QUALITY INSPECTION
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