5 results about "Dioxetane" patented technology

This invention discloses a microRNA-responsive 1,2-dioxane-based chemiluminescent compound, its dual-probe system, kit, synthesis method, and applications. The compound's core is an o-acrylate-substituted phenoxyadamantane-1,2-dioxane, with the phenolic hydroxyl group protected by an azide benzyl group. The two-component probe system constructed based on this compound comprises an azide-modified first probe and a phosphine-modified second probe, each complementary to different segments of the target microRNA. Hybridization brings the two probes closer, triggering a Staudinger reaction to remove the protecting group and activate the chemiluminescent signal. This probe system requires no enzyme amplification or nucleic acid extraction, achieves a detection limit of 1.37 fM, and a signal-to-noise ratio as high as 157-fold. It can be successfully used for real-time imaging of endogenous microRNA-21 in live cells, showing broad application prospects in early tumor screening, auxiliary diagnosis, and prognostic assessment.

Described herein are 1,2-dioxetane dimers that are useful as chemiluminescent probes, diagnostic agents, and imaging agents. Also described herein are compositions containing such compounds and methods of using the same.

This invention relates to a class of dihydroxanthine chemiluminescent groups, their preparation methods, and applications. The dihydroxanthine chemiluminescent group developed in this invention, compared to the traditional phenolic dioxane chemiluminescent group which uses two rigid six-membered rings to fix the double bond conformation, exhibits a higher luminescence quantum yield and a longer emission wavelength. More importantly, it possesses a reactive aldehyde group, which, while maintaining high rigidity, extends the π-electron conjugated system, causing its emission wavelength to redshift to the near-infrared region. This allows it to be extended to chemiluminescence detection and in vivo imaging in small animal models.

Described herein are 1,2-dioxetane dimers that are useful as chemiluminescent probes, diagnostic agents, and imaging agents. Also described herein are compositions containing such compounds and methods of using the same.